Difference: 1230CheckList (1 vs. 6)

Revision 603 Mar 2010 - Main.DavidStokes

 
META TOPICPARENT name="CryoEM"

1230 Robot checklist

Added:
>
>

Robot

  1. turn on gas tank
  2. start iRobot and hist "Start"
  3. demagnitize grid pickup nozzle
  4. clamp grid tray in position
  5. place EM holder on tray

Microscope

  1. grease o-ring on holder
 
  1. check beam
Added:
>
>
  1. calibrate goniometer
 
  1. center condensor aperture
  2. remove objective apeture
Deleted:
<
<
  1. correct bright/dark field once a week
 
  1. insert camera
  2. airlock toggle switch to "Robot"
Deleted:
<
<
  1. calibrate goniometer
 
  1. move goniometer to "home" position
Changed:
<
<
  1. grease o-ring on holder
>
>
  1. correct bright/dark field once a week
Deleted:
<
<
  1. place holder on tray
  2. demagnitize grid pickup nozzle
  3. clamp grid tray in position
  4. turn on gas tank
 

Revision 503 Mar 2010 - Main.ChangkiKim

 
META TOPICPARENT name="CryoEM"

1230 Robot checklist

  1. check beam
  2. center condensor aperture
  3. remove objective apeture
Changed:
<
<
  1. Correct bright/dark field once a week
>
>
  1. correct bright/dark field once a week
 
  1. insert camera
  2. airlock toggle switch to "Robot"
  3. calibrate goniometer
  4. move goniometer to "home" position
  5. grease o-ring on holder
  6. place holder on tray
  7. demagnitize grid pickup nozzle
  8. clamp grid tray in position
  9. turn on gas tank

Revision 403 Mar 2010 - Main.ChangkiKim

 
META TOPICPARENT name="CryoEM"

1230 Robot checklist

  1. check beam
  2. center condensor aperture
  3. remove objective apeture
Added:
>
>
  1. Correct bright/dark field once a week
 
  1. insert camera
  2. airlock toggle switch to "Robot"
  3. calibrate goniometer
  4. move goniometer to "home" position
  5. grease o-ring on holder
  6. place holder on tray
  7. demagnitize grid pickup nozzle
  8. clamp grid tray in position
  9. turn on gas tank

Revision 321 Jan 2010 - Main.DavidStokes

 
META TOPICPARENT name="CryoEM"
Changed:
<
<
>
>

1230 Robot checklist

Deleted:
<
<

1230 Robot checklist for

 
  1. check beam
Changed:
<
<
  1. check condensor aperture
>
>
  1. center condensor aperture
 
  1. remove objective apeture
  2. insert camera
  3. airlock toggle switch to "Robot"
  4. calibrate goniometer
  5. move goniometer to "home" position
  6. grease o-ring on holder
  7. place holder on tray
  8. demagnitize grid pickup nozzle
  9. clamp grid tray in position
  10. turn on gas tank
Changed:
<
<
>
>
 

Revision 221 Jan 2010 - Main.DavidStokes

 
META TOPICPARENT name="CryoEM"
Added:
>
>

1230 Robot checklist for

  1. check beam
  2. check condensor aperture
  3. remove objective apeture
  4. insert camera
  5. airlock toggle switch to "Robot"
  6. calibrate goniometer
  7. move goniometer to "home" position
  8. grease o-ring on holder
  9. place holder on tray
  10. demagnitize grid pickup nozzle
  11. clamp grid tray in position
  12. turn on gas tank
 
Deleted:
<
<

1230 checklist

  1. Make sure switch is in desired position "Robot" or "Manual"
  2. Home stage
    • specimen/position/positon 1
  3. Calibrate Stage
    • after insertion of holder
    • specimen/calibration
 
Changed:
<
<
>
>

Revision 121 Sep 2009 - Main.KdDerr

 
META TOPICPARENT name="CryoEM"

1230 checklist

  1. Make sure switch is in desired position "Robot" or "Manual"
  2. Home stage
    • specimen/position/positon 1
  3. Calibrate Stage
    • after insertion of holder
    • specimen/calibration

  • Set ALLOWTOPICVIEW =

-- KdDerr - 21 Sep 2009

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