Difference: 2dxEmrE (1 vs. 17)

Revision 1703 Apr 2014 - Main.NicolasCoudray

Changed:
<
<
META TOPICPARENT name="2DCrystallizationExp"
>
>
META TOPICPARENT name="TwoDCrystallizationGroup"
 

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (X71)

Protocol

About the protein: see the Protein acquisition form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Set 2nd June. Stopped 14th June.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307132236" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda_EmrE_D5-Protein_acquisition_form.doc" size="55808" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" user="Main.NicolasCoudray" version="6"

Revision 1617 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Changed:
<
<

Experiment of Mai 2011 (EM.174 - X67)

>
>

Experiment of Mai 2011 (X71)

 

Protocol

About the protein: see the Protein acquisition form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Added:
>
>		Set 2nd June. Stopped 14th June.
 

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307132236" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda_EmrE_D5-Protein_acquisition_form.doc" size="55808" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" user="Main.NicolasCoudray" version="6"

Revision 1503 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

Added:
>
>		About the protein: see the Protein acquisition form.
 conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Deleted:
<
<		 * Nanda_EmrE_D5-Protein_acquisition_form.doc: Nanda_EmrE_D5-Protein_acquisition_form.doc
 
Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="6"
META FILEATTACHMENT attachment="Nanda_EmrE_D5-Protein_acquisition_form.doc" attr="" comment="" date="1307132235" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda EmrE D5-Protein acquisition form.doc" size="55808" stream="Nanda EmrE D5-Protein acquisition form.doc" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307132236" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda_EmrE_D5-Protein_acquisition_form.doc" size="55808" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" user="Main.NicolasCoudray" version="6"
 

Revision 1403 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Added:
>
>		 * Nanda_EmrE_D5-Protein_acquisition_form.doc: Nanda_EmrE_D5-Protein_acquisition_form.doc
 
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="6"
Added:
>
>
META FILEATTACHMENT attachment="Nanda_EmrE_D5-Protein_acquisition_form.doc" attr="" comment="" date="1307132235" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda EmrE D5-Protein acquisition form.doc" size="55808" stream="Nanda EmrE D5-Protein acquisition form.doc" user="Main.NicolasCoudray" version="1"
 

Revision 1303 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
Changed:
<
<
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
>
>
  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
 
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="6"

Revision 1202 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
Changed:
<
<
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
>
>
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
 
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
Changed:
<
<
  • dialysis block, each lipid at least once in cassette to sample and also with Biobeads; If enough protein, try with CD (1:1 to 5:1 ratio?)
>
>
  • dialysis block, each lipid at least once in cassette to sample
 See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="6"

Revision 1102 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample and also with Biobeads; If enough protein, try with CD (1:1 to 5:1 ratio?)

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307038287" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="118272" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="5"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="6"
 

Revision 1002 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample and also with Biobeads; If enough protein, try with CD (1:1 to 5:1 ratio?)

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307033652" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="118784" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="4"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307038287" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="118272" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="5"
 

Revision 902 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample and also with Biobeads; If enough protein, try with CD (1:1 to 5:1 ratio?)

See Protocol_EmrE_2011_Mai.xls for details.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1306955468" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="167936" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="3"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1307033652" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="118784" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="4"
 

Revision 802 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
Changed:
<
<
  • Lipids prepared in DDM: DMPC, E.Coli and EyPC:PA (9:1 ratio)
>
>
  • Dialysis buffer: 20mM sodium phosphate pH7, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
 
  • pH: 7 and 8
Changed:
<
<
  • Ion: with and without TPP+ in the buffer
>
>
  • Lipids prepared in DM:
Added:
>
>
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
 
  • Temperature: 25 degrees
  • LPR: those usually used
Added:
>
>
  • dialysis block, each lipid at least once in cassette to sample and also with Biobeads; If enough protein, try with CD (1:1 to 5:1 ratio?)
 
Deleted:
<
<		Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.
 See Protocol_EmrE_2011_Mai.xls for details.
Added:
>
>

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)
 
Added:
>
>
 
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1306955468" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="167936" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="3"

Revision 701 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EyPC:PA (9:1 ratio)
  • pH: 7 and 8
  • Ion: with and without TPP+ in the buffer
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

See Protocol_EmrE_2011_Mai.xls for details.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305830149" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="171008" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="2"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1306955468" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="167936" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="3"
 

Revision 627 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
Changed:
<
<
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
>
>
  • Lipids prepared in DDM: DMPC, E.Coli and EyPC:PA (9:1 ratio)
 
  • pH: 7 and 8
  • Ion: with and without TPP+ in the buffer
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

See Protocol_EmrE_2011_Mai.xls for details.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305830149" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="171008" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="2"

Revision 519 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
  • pH: 7 and 8
  • Ion: with and without TPP+ in the buffer
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

See Protocol_EmrE_2011_Mai.xls for details.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305829964" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="186880" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305830149" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="171008" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="2"
 

Revision 419 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
  • pH: 7 and 8
  • Ion: with and without TPP+ in the buffer
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

Added:
>
>		See Protocol_EmrE_2011_Mai.xls for details.
 
Deleted:
<
<
 
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Changed:
<
<		 * Protocol_EmrE_2011_Mai.xls: Protocol_EmrE_2011_Mai.xls
>
>
 
META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305829964" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="186880" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="1"

Revision 319 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
  • pH: 7 and 8
  • Ion: with and without TPP+ in the buffer
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Added:
>
>		 * Protocol_EmrE_2011_Mai.xls: Protocol_EmrE_2011_Mai.xls

META FILEATTACHMENT attachment="Protocol_EmrE_2011_Mai.xls" attr="" comment="" date="1305829964" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="186880" stream="Protocol_EmrE_2011_Mai.xls" user="Main.NicolasCoudray" version="1"
 

Revision 219 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
  • pH: 7 and 8
Changed:
<
<
  • Ion: with and without TPP+
>
>
  • Ion: with and without TPP+ in the buffer
 
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

Revision 119 May 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (EM.174 - X67)

Protocol

conditions to screen:
  • Lipids prepared in DDM: DMPC, E.Coli and EggYolk
  • pH: 7 and 8
  • Ion: with and without TPP+
  • Temperature: 25 degrees
  • LPR: those usually used

Do it in the dialysis block and a few in biobeads at 4 degrees for an overnight test. Then, use it also to test the sucrose gradient method.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

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