Difference: 2dxEmrE (15 vs. 16)

Revision 1617 Jun 2011 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - EmrE 2D-crystallization trials

Changed:

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Experiment of Mai 2011 (EM.174 - X67)

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Experiment of Mai 2011 (X71)

Protocol

About the protein: see the Protein acquisition form.

conditions to screen:

Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
pH: 7 and 8
Lipids prepared in DM:
- DMPC
- E.Coli
- EyPC:PA (9:1 ratio),
- DOPG
- POPC
Temperature: 25 degrees
LPR: those usually used
dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Added:

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Set 2nd June. Stopped 14th June.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

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META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" user="Main.NicolasCoudray" version="6"

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