Difference: 2dxEmrE (16 vs. 17)

Revision 1703 Apr 2014 - Main.NicolasCoudray

Changed:
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META TOPICPARENT name="2DCrystallizationExp"
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META TOPICPARENT name="TwoDCrystallizationGroup"
 

2dx - EmrE 2D-crystallization trials

Experiment of Mai 2011 (X71)

Protocol

About the protein: see the Protein acquisition form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DM:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block, each lipid at least once in cassette to sample

See Protocol_EmrE_2011_Mai.xls for details.

Set 2nd June. Stopped 14th June.

Comments (June 2nd meeting)

Later, ligands will be used (TPP+ in the buffer: 20 uM or 100 uM as substrate for the protein, at pH=8 only)

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 May 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307132236" name="Nanda_EmrE_D5-Protein_acquisition_form.doc" path="Nanda_EmrE_D5-Protein_acquisition_form.doc" size="55808" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1307069110" name="Protocol_EmrE_2011_Mai.xls" path="Protocol_EmrE_2011_Mai.xls" size="115712" user="Main.NicolasCoudray" version="6"
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