Difference: 2dxEmrEShewanellaOneidensis (1 vs. 20)

Revision 2003 Apr 2014 - Main.NicolasCoudray

Changed:
<
<
META TOPICPARENT name="2DCrystallizationExp"
>
>
META TOPICPARENT name="TwoDCrystallizationGroup"
 

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Properties

  • Amino acid sequence:

M S W L L L I L A G L F E I G W A I G L K Y T D G F T R L W P S V F T V L S M A L S V L L L G L A V K Q L P I G T A Y G V W V G I G A M G T A I A G I I L L G E G V S L L K I A S L I L I L L G V L G L K L A H A E N L Y F Q G

  • 112 amino acids
  • 11.8 kDa, Expasy (10.9 kDa, Nanda)
  • pI ~ 8.09
  • 4 negatively charged residues
  • 5 positively charged residues
  • extinction coefficient: 26470
  • instability index: 19.76
  • alipathic index: 159.38
  • GRAVY: 1.298
  • TM helices: 4
  • amino acids in the TM domain: 82 (LPR(m/m) expected: 1-40 ~ 0.03 to 1.4 in w/w; with LPR(m/m)=24>>LPR(w/w)=0.68; LPR(m/m)=5>>LPR(w/w)=0.14; LPR(m/m)=40>>LPR(w/w)=1.12;)

Experiment of December 2011 (X94)

About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.

Set Dec 15th.

Comments (Jan 2012)

See image selection: X94-EmrE_Soneid-ImageSelection.pptx: X94-EmrE_Soneid-ImageSelection.pptx

Controls are ok, but no good results (aggregates, tiny vesicles, or grids mostly empty).

CD will be tried next time. If results are still bad, then the protein will be dropped.

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bad -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1324046222" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="472952" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1326148224" name="X94-EmrE_Soneid-ImageSelection.pptx" path="X94-EmrE_Soneid-ImageSelection.pptx" size="22856602" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1924 Jan 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Properties

  • Amino acid sequence:

M S W L L L I L A G L F E I G W A I G L K Y T D G F T R L W P S V F T V L S M A L S V L L L G L A V K Q L P I G T A Y G V W V G I G A M G T A I A G I I L L G E G V S L L K I A S L I L I L L G V L G L K L A H A E N L Y F Q G

  • 112 amino acids
  • 11.8 kDa, Expasy (10.9 kDa, Nanda)
  • pI ~ 8.09
  • 4 negatively charged residues
  • 5 positively charged residues
  • extinction coefficient: 26470
  • instability index: 19.76
  • alipathic index: 159.38
  • GRAVY: 1.298
  • TM helices: 4
  • amino acids in the TM domain: 82 (LPR(m/m) expected: 1-40 ~ 0.03 to 1.4 in w/w; with LPR(m/m)=24>>LPR(w/w)=0.68; LPR(m/m)=5>>LPR(w/w)=0.14; LPR(m/m)=40>>LPR(w/w)=1.12;)

Experiment of December 2011 (X94)

About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.

Set Dec 15th.

Comments (Jan 2012)

Added:
>
>		See image selection: X94-EmrE_Soneid-ImageSelection.pptx: X94-EmrE_Soneid-ImageSelection.pptx
 Controls are ok, but no good results (aggregates, tiny vesicles, or grids mostly empty).
Changed:
<
<		See image selection: X94-EmrE_Soneid-ImageSelection.pptx: X94-EmrE_Soneid-ImageSelection.pptx
>
>		CD will be tried next time. If results are still bad, then the protein will be dropped.
Added:
>
>
 

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bad -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1324046222" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="472952" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1326148224" name="X94-EmrE_Soneid-ImageSelection.pptx" path="X94-EmrE_Soneid-ImageSelection.pptx" size="22856602" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1809 Jan 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Properties

  • Amino acid sequence:

M S W L L L I L A G L F E I G W A I G L K Y T D G F T R L W P S V F T V L S M A L S V L L L G L A V K Q L P I G T A Y G V W V G I G A M G T A I A G I I L L G E G V S L L K I A S L I L I L L G V L G L K L A H A E N L Y F Q G

  • 112 amino acids
  • 11.8 kDa, Expasy (10.9 kDa, Nanda)
  • pI ~ 8.09
  • 4 negatively charged residues
  • 5 positively charged residues
  • extinction coefficient: 26470
  • instability index: 19.76
  • alipathic index: 159.38
  • GRAVY: 1.298
  • TM helices: 4
  • amino acids in the TM domain: 82 (LPR(m/m) expected: 1-40 ~ 0.03 to 1.4 in w/w; with LPR(m/m)=24>>LPR(w/w)=0.68; LPR(m/m)=5>>LPR(w/w)=0.14; LPR(m/m)=40>>LPR(w/w)=1.12;)

Experiment of December 2011 (X94)

About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.

Set Dec 15th.

Added:
>
>

Comments (Jan 2012)

Controls are ok, but no good results (aggregates, tiny vesicles, or grids mostly empty).
 
Added:
>
>		See image selection: X94-EmrE_Soneid-ImageSelection.pptx: X94-EmrE_Soneid-ImageSelection.pptx
 

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

Added:
>
>
 

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
Changed:
<
<
  • Yiip control are bas -> do experiments to find out why...
>
>
  • Yiip control are bad -> do experiments to find out why...
 

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1324046222" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="472952" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1326148224" name="X94-EmrE_Soneid-ImageSelection.pptx" path="X94-EmrE_Soneid-ImageSelection.pptx" size="22856602" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1709 Jan 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Added:
>
>

Properties

  • Amino acid sequence:

M S W L L L I L A G L F E I G W A I G L K Y T D G F T R L W P S V F T V L S M A L S V L L L G L A V K Q L P I G T A Y G V W V G I G A M G T A I A G I I L L G E G V S L L K I A S L I L I L L G V L G L K L A H A E N L Y F Q G

  • 112 amino acids
  • 11.8 kDa, Expasy (10.9 kDa, Nanda)
  • pI ~ 8.09
  • 4 negatively charged residues
  • 5 positively charged residues
  • extinction coefficient: 26470
  • instability index: 19.76
  • alipathic index: 159.38
  • GRAVY: 1.298
  • TM helices: 4
  • amino acids in the TM domain: 82 (LPR(m/m) expected: 1-40 ~ 0.03 to 1.4 in w/w; with LPR(m/m)=24>>LPR(w/w)=0.68; LPR(m/m)=5>>LPR(w/w)=0.14; LPR(m/m)=40>>LPR(w/w)=1.12;)
 

Experiment of December 2011 (X94)

About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.

Set Dec 15th.

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bas -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1324046222" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="472952" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1616 Dec 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Changed:
<
<

Experiment of June 2011 (X94)

>
>

Experiment of December 2011 (X94)

 About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.
Added:
>
>		Set Dec 15th.
 

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bas -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
Changed:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981594" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="470710" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1324046222" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="472952" user="Main.NicolasCoudray" version="2"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1515 Dec 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Added:
>
>

Experiment of June 2011 (X94)

About the protein: see the S._Oneidensis_Protein_acquisition_form_12.15.11. Protein in 0.2%DDM,20mM Hepes,200mM NaCl?, 5% Glycerol, pH 7.4.
  • 5 Lipids in 5 detergent screening
  • Buffer: pH8, 20mM sodium phosphate, 100mM NaCl?. 2mM MgCl?, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • Temperature: 25 degrees
  • microdialysis plate
Details: X94-EmrE_Oneidensis.xlsx.

 

Experiment of June 2011 (X72-EM.194)

Protocol

About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bas -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Added:
>
>
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981594" name="X94-EmrE_Oneidensis.xlsx" path="X94-EmrE_Oneidensis.xlsx" size="470710" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1323981601" name="S._Oneidensis_Protein_acquisition_form_12.15.11" path="S._Oneidensis_Protein_acquisition_form_12.15.11" size="56832" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1418 Oct 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Changed:
<
<

Experiment of June 2011

>
>

Experiment of June 2011 (X72-EM.194)

 

Protocol

Changed:
<
<		About the protein: see the Protein Acquisition Form.
>
>		About the protein: see the Protein Acquisition Form. And for the purification gels... : S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt.
 conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bas -> do experiments to find out why...

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Added:
>
>

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1318962132" name="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" path="S.Oneidensis_Purification_for_1st_2D_6.24.11.ppt" size="293888" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1321 Jul 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the Protein Acquisition Form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

2 weeks (Set 24th June; collected July 8th)

Added:
>
>

July 21th Meeting - comments

  • All is aggregated.
  • Controls of protein only and lipids only are OK
  • Yiip control are bas -> do experiments to find out why...
 

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1211 Jul 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the Protein Acquisition Form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Changed:
<
<		Set 24th June.
>
>		2 weeks (Set 24th June; collected July 8th)
 

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1130 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the Protein Acquisition Form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Changed:
<
<		Set 23rd June.
>
>		Set 24th June.
 

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 1024 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the Protein Acquisition Form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
Changed:
<
<
  • dialysis block + some duplicated in cassette to sample
>
>
  • dialysis block
 See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="3"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" user="Main.NicolasCoudray" version="3"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 924 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the Protein Acquisition Form.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Changed:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308958728" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" user="Main.NicolasCoudray" version="2"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308960899" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="129024" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="3"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"

Revision 824 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

Changed:
<
<		About the protein: see the ???? Protein Acquisition Form?????.
>
>		About the protein: see the Protein Acquisition Form.
 conditions to screen:
  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Deleted:
<
<		 * S._Oneidensis_Protein_acquisition_form_6.24.11.doc: S._Oneidensis_Protein_acquisition_form_6.24.11.doc
 
Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308958727" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attachment="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" attr="" comment="" date="1308959122" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S. Oneidensis Protein acquisition form 6.24.11.doc" size="56320" stream="S. Oneidensis Protein acquisition form 6.24.11.doc" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308958728" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" user="Main.NicolasCoudray" version="2"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1308959123" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" size="56320" user="Main.NicolasCoudray" version="1"
 

Revision 724 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • POPC (C12E8 only)
  • Temperature: 25 degrees
  • LPR: 0.5 to 1
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Added:
>
>		 * S._Oneidensis_Protein_acquisition_form_6.24.11.doc: S._Oneidensis_Protein_acquisition_form_6.24.11.doc
 
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308958727" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="2"
Added:
>
>
META FILEATTACHMENT attachment="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" attr="" comment="" date="1308959122" name="S._Oneidensis_Protein_acquisition_form_6.24.11.doc" path="S. Oneidensis Protein acquisition form 6.24.11.doc" size="56320" stream="S. Oneidensis Protein acquisition form 6.24.11.doc" user="Main.NicolasCoudray" version="1"
 

Revision 624 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
Changed:
<
<
    • EyPC:PA (9:1 ratio),
>
>
    • POPC (C12E8 only)
Deleted:
<
<
    • DOPG
    • POPC
 
  • Temperature: 25 degrees
Changed:
<
<
  • LPR: those usually used
>
>
  • LPR: 0.5 to 1
 
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308958727" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="2"

Revision 524 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Changed:
<
<
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308856622" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="147456" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308958727" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="112128" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="2"
 

Revision 423 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block + some duplicated in cassette to sample

See Protocol_EmrE_oneidensis_2011_June.xls for details.

Set 23rd June.

Changed:
<
<		--
>
>
 
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308856622" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="147456" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="1"

Revision 323 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block + some duplicated in cassette to sample
Changed:
<
<		See for details.
>
>		See Protocol_EmrE_oneidensis_2011_June.xls for details.
 Set 23rd June.

--

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Changed:
<
<		 * Protocol_EmrE_oneidensis_2011_June.xls: Protocol_EmrE_oneidensis_2011_June.xls
>
>
 
META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308856622" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="147456" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="1"

Revision 223 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block + some duplicated in cassette to sample

See for details.

Set 23rd June.

--

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

Added:
>
>		 * Protocol_EmrE_oneidensis_2011_June.xls: Protocol_EmrE_oneidensis_2011_June.xls

META FILEATTACHMENT attachment="Protocol_EmrE_oneidensis_2011_June.xls" attr="" comment="" date="1308856622" name="Protocol_EmrE_oneidensis_2011_June.xls" path="Protocol_EmrE_oneidensis_2011_June.xls" size="147456" stream="Protocol_EmrE_oneidensis_2011_June.xls" user="Main.NicolasCoudray" version="1"
 

Revision 123 Jun 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - EmrE (Shewanella Oneidensis) 2D-crystallization trials

Experiment of June 2011

Protocol

About the protein: see the ???? Protein Acquisition Form?????.

conditions to screen:

  • Dialysis buffer: 20mM sodium phosphate, 100mM NaCl. 2mM MgCl, 1mM EDTA, 4mM DTT, 4mM mercapto-ethanol, 5mM sodium azide.
  • pH: 7 and 8
  • Lipids prepared in DDM and C12E8:
    • DMPC
    • E.Coli
    • EyPC:PA (9:1 ratio),
    • DOPG
    • POPC
  • Temperature: 25 degrees
  • LPR: those usually used
  • dialysis block + some duplicated in cassette to sample

See for details.

Set 23rd June.

--

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 23 Jun 2011

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