Difference: 2dx_SFV (1 vs. 7)

Revision 703 Apr 2014 - Main.NicolasCoudray

Changed:
<
<
META TOPICPARENT name="2DCrystallizationExp"
>
>
META TOPICPARENT name="TwoDCrystallizationGroup"
 

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files (Martin's archives):

-- NicolasCoudray - 20 Jan 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565328" name="2009_07_1_X31-SFV-screen_conditions.doc" path="2009_07_1_X31-SFV-screen_conditions.doc" size="38400" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein_acquisition_form(2).doc" size="54784" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images_of_SFV.ppt" size="7007232" user="Main.NicolasCoudray" version="1"

Revision 620 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files (Martin's archives):

Changed:
<
<		 * 2009_07_1_Images_of_SFV.ppt: images selection * 2009_07_1_Protein_acquisition_form(2).doc: Protein acquisition form * 2009_07_1_X31-SFV-screen_conditions.doc: SFV screen conditions and scoring overview
>
>
 

-- NicolasCoudray - 20 Jan 2011

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565328" name="2009_07_1_X31-SFV-screen_conditions.doc" path="2009_07_1_X31-SFV-screen_conditions.doc" size="38400" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein_acquisition_form(2).doc" size="54784" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images_of_SFV.ppt" size="7007232" user="Main.NicolasCoudray" version="1"

Revision 520 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?
Changed:
<
<		Associated files:
>
>		Associated files (Martin's archives):
Added:
>
>		 * 2009_07_1_Images_of_SFV.ppt: images selection * 2009_07_1_Protein_acquisition_form(2).doc: Protein acquisition form * 2009_07_1_X31-SFV-screen_conditions.doc: SFV screen conditions and scoring overview
 

Deleted:
<
<		-- NicolasCoudray - 20 Jan 2011 * 2009_07_1_Images_of_SFV.ppt: 2009_07_1_Images_of_SFV.ppt
 
Deleted:
<
<		 * 2009_07_1_Protein_acquisition_form(2).doc: 2009_07_1_Protein_acquisition_form(2).doc
 
Changed:
<
<		 * 2009_07_1_X31-SFV-screen_conditions.doc: 2009_07_1_X31-SFV-screen_conditions.doc
>
>		-- NicolasCoudray - 20 Jan 2011
 
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565328" name="2009_07_1_X31-SFV-screen_conditions.doc" path="2009_07_1_X31-SFV-screen_conditions.doc" size="38400" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein_acquisition_form(2).doc" size="54784" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images_of_SFV.ppt" size="7007232" user="Main.NicolasCoudray" version="1"
Deleted:
<
<
META FILEATTACHMENT attachment="2009_07_1_X31-SFV-screen_conditions.doc" attr="" comment="" date="1295565328" name="2009_07_1_X31-SFV-screen_conditions.doc" path="2009_07_1_X31-SFV-screen conditions.doc" size="38400" stream="2009_07_1_X31-SFV-screen conditions.doc" user="Main.NicolasCoudray" version="1"
 

Revision 420 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files:

-- NicolasCoudray - 20 Jan 2011 * 2009_07_1_Images_of_SFV.ppt: 2009_07_1_Images_of_SFV.ppt

* 2009_07_1_Protein_acquisition_form(2).doc: 2009_07_1_Protein_acquisition_form(2).doc

Changed:
<
<
META FILEATTACHMENT attachment="2009_07_1_Images_of_SFV.ppt" attr="" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images of SFV.ppt" size="7007232" stream="2009_07_1_Images of SFV.ppt" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attachment="2009_07_1_Protein_acquisition_form(2).doc" attr="" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein acquisition form(2).doc" size="54784" stream="2009_07_1_Protein acquisition form(2).doc" user="Main.NicolasCoudray" version="1"
>
>		 * 2009_07_1_X31-SFV-screen_conditions.doc: 2009_07_1_X31-SFV-screen_conditions.doc
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein_acquisition_form(2).doc" size="54784" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images_of_SFV.ppt" size="7007232" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attachment="2009_07_1_X31-SFV-screen_conditions.doc" attr="" comment="" date="1295565328" name="2009_07_1_X31-SFV-screen_conditions.doc" path="2009_07_1_X31-SFV-screen conditions.doc" size="38400" stream="2009_07_1_X31-SFV-screen conditions.doc" user="Main.NicolasCoudray" version="1"
 

Revision 320 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files:

-- NicolasCoudray - 20 Jan 2011 * 2009_07_1_Images_of_SFV.ppt: 2009_07_1_Images_of_SFV.ppt

Added:
>
>		 * 2009_07_1_Protein_acquisition_form(2).doc: 2009_07_1_Protein_acquisition_form(2).doc
 
META FILEATTACHMENT attachment="2009_07_1_Images_of_SFV.ppt" attr="" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images of SFV.ppt" size="7007232" stream="2009_07_1_Images of SFV.ppt" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attachment="2009_07_1_Protein_acquisition_form(2).doc" attr="" comment="" date="1295565315" name="2009_07_1_Protein_acquisition_form(2).doc" path="2009_07_1_Protein acquisition form(2).doc" size="54784" stream="2009_07_1_Protein acquisition form(2).doc" user="Main.NicolasCoudray" version="1"
 

Revision 220 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files:

-- NicolasCoudray - 20 Jan 2011

Added:
>
>		 * 2009_07_1_Images_of_SFV.ppt: 2009_07_1_Images_of_SFV.ppt

META FILEATTACHMENT attachment="2009_07_1_Images_of_SFV.ppt" attr="" comment="" date="1295565300" name="2009_07_1_Images_of_SFV.ppt" path="2009_07_1_Images of SFV.ppt" size="7007232" stream="2009_07_1_Images of SFV.ppt" user="Main.NicolasCoudray" version="1"
 

Revision 120 Jan 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - SFV 2D-crystallization

Contents

Overview

Experiments (date of protein delivery)

July 1st 2009

Screen reference: X31

Conditions of best results (diffracting crystals):

  • LPR:0.5
  • Lipid Mixture: 17% DOPC, 17%DOPE, 17%sphigomyelin, 50%Cholesterol
  • Buffer: 50mM Tris, pH8.5, 20mM MgCl2, 5mM NaN3?

Associated files:

-- NicolasCoudray - 20 Jan 2011

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