Difference: BiomekNXProbot (1 vs. 2)

Revision 209 Jun 2015 - Main.NicolasCoudray

 
META TOPICPARENT name="TemimpsGroup"

Biomek NXP robot

Contents

Before each use

  • Empty the "Waste" bottle if it is almost full
  • Check the level of water in the "Source" bottle. It should be well above the tips. Otherwise:
    • fill it with de-gazed distilled water (for de-gazing, fill 2L bottles with distilled water and let them open overnight)
  • Clean the device:
    • "Instrument >> Manual Control"
    • ">> Advanced Control >> Pod1"
    • First, click on "Purge system"
    • Then, click on "Purge Syringes"
Added:
>
>		Note:
  • Check that the deck should be "NYSB"
 

Use

  • Open biomek software
  • Start with a project:
    • open an existing project: "Project >> open projet >> ". Two projects of interest:
      • micro plate
      • default project
    • or create a new project: "Project >> new projet >> "
  • Start a program:
    • open an existing program. Programs of interest:
      • micro dialysis plate (in the microplate project)
      • stainingP_8ul_0.25mg/ml_40s_for_whole_grids_disposed_Tipsb (in the default project)
    • or create a new program: "File >> New"
      • On the left hand-side, icons can be drag and drop in the command window. "Instrument set up", "Transfer" and "loop" are the 3 main icons to be used.
      • For each component dropped in the command window, on the right hand-side, its properties can be set
        • information about the instrument and positions of the plates, and details about which plate is used. In the "Project >> Labware or Tip tape editor", specific information about the plates and the tips can be found (AA_Span_8_200ul or 20ul tips are used for instance)
      • For "instrument set up", plates must be dragged and dropped on the scheme below to say which plates are used and where they are located in the robot.
      • For the "tranfer" tool, set the source, select it and its destination
  • Run the program. Usually 2 warning appear after you click run. Ignore the 2 warnings.
Added:
>
>

Reframe the deck

To do if the tips are misaligned with the deck even after homing the axis:
  • Place AccuFrame on the different pods
  • Use Probe1 (replace the outer ... by the metallic one) for all decks except the front ones
  • Intrument >> Deck Editor >> double click on current deck (P13 for example)
    • Click "Auto Teach" (Probe 7/1)
    • Click OK
    • If the tip fails to enter the AccuFrame, move it manually within it and press "Teach" instead of "Auto Teach"

Backup

Save:
  • the project file:
    • project>export project
  • the deck
    • instrument>Import/export utility.
    • Highlight deck layouts and then click on export. You should see the folder move to the right hand side.
    • Click on save and save it to the desktop also.
  • method
    • Then open the method and go to file>export and save to desktop also.
  • instrument file
    • instrument>save instrument as.
 

Trouble shooting

If you abort a program in the middle, move carefully the tips up (do not hold the lower parts of the tips which are very fragile), and do "Instrument > Home".

Sometimes, some errors messages keep coming back. Restart the software if necessary, or if not enough, the restart the computer.

In case of major issues with the robot, the phone number of the company is written on the robot, the extension of the contact (Tim) in the book in the drawer below the computer.

Added:
>
>		To change the overall speed:
  • Instrument >> Hardware Setup >> Pod1 >> Add Pod Setting >> Speed Limit
 
Added:
>
>
 
Deleted:
<
<		d80 1
 
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 26 Aug 2011

Revision 126 Aug 2011 - Main.NicolasCoudray

 
META TOPICPARENT name="TemimpsGroup"

Biomek NXP robot

Contents

Before each use

  • Empty the "Waste" bottle if it is almost full
  • Check the level of water in the "Source" bottle. It should be well above the tips. Otherwise:
    • fill it with de-gazed distilled water (for de-gazing, fill 2L bottles with distilled water and let them open overnight)
  • Clean the device:
    • "Instrument >> Manual Control"
    • ">> Advanced Control >> Pod1"
    • First, click on "Purge system"
    • Then, click on "Purge Syringes"

Use

  • Open biomek software
  • Start with a project:
    • open an existing project: "Project >> open projet >> ". Two projects of interest:
      • micro plate
      • default project
    • or create a new project: "Project >> new projet >> "
  • Start a program:
    • open an existing program. Programs of interest:
      • micro dialysis plate (in the microplate project)
      • stainingP_8ul_0.25mg/ml_40s_for_whole_grids_disposed_Tipsb (in the default project)
    • or create a new program: "File >> New"
      • On the left hand-side, icons can be drag and drop in the command window. "Instrument set up", "Transfer" and "loop" are the 3 main icons to be used.
      • For each component dropped in the command window, on the right hand-side, its properties can be set
        • information about the instrument and positions of the plates, and details about which plate is used. In the "Project >> Labware or Tip tape editor", specific information about the plates and the tips can be found (AA_Span_8_200ul or 20ul tips are used for instance)
      • For "instrument set up", plates must be dragged and dropped on the scheme below to say which plates are used and where they are located in the robot.
      • For the "tranfer" tool, set the source, select it and its destination
  • Run the program. Usually 2 warning appear after you click run. Ignore the 2 warnings.

Trouble shooting

If you abort a program in the middle, move carefully the tips up (do not hold the lower parts of the tips which are very fragile), and do "Instrument > Home".

Sometimes, some errors messages keep coming back. Restart the software if necessary, or if not enough, the restart the computer.

In case of major issues with the robot, the phone number of the company is written on the robot, the extension of the contact (Tim) in the book in the drawer below the computer.

d80 1

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 26 Aug 2011

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