Difference: CarbonCoating (1 vs. 11)

Revision 1118 Sep 2009 - Main.KdDerr

 
META TOPICPARENT name="CemProtoc"
Changed:
<
<

Carbon Coating

>
>		Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM
Added:
>
>

Principles & Protocols

Principles - Carbon Coating

Jump to Protocol

 The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 1026 Aug 2009 - Main.KdDerr

Changed:
<
<
META TOPICPARENT name="HowtoGridSelection"
>
>
META TOPICPARENT name="CemProtoc"
 

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 919 Aug 2009 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Deleted:
<
<		Contents
 

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 817 Sep 2008 - Main.DavidCowburn

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

Changed:
<
<		The best method to use for carbon coating is an on going debate. Best to Trash.findDFdf a method that works and stick to it.
>
>		The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.
 

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 717 Sep 2008 - Main.DavidCowburn

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to Trash.findDFdf a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 616 Jul 2008 - Main.DavidCowburn

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

Changed:
<
<		The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.
>
>		The best method to use for carbon coating is an on going debate. Best to Trash.findDFdf a method that works and stick to it.
 

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 531 Aug 2007 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
Added:
>
>
  • carbon rod sharpening angles:
    carbpts.jpg
 
Deleted:
<
<
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

Changed:
<
<		 * carbon rod sharpening angles:
>
>
Deleted:
<
<		 carbpts.jpg
 
META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"

Revision 431 Aug 2007 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

Added:
>
>		 * carbon rod sharpening angles:
carbpts.jpg

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"
 

Revision 331 Aug 2007 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

Revision 231 Aug 2007 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
Changed:
<
<
  • Graphite vs. pure carbon debate
>
>
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
 
  • Flat vs. pointed rod debate

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

Revision 129 Aug 2007 - Main.KdDerr

 
META TOPICPARENT name="HowtoGridSelection"
Contents

Carbon Coating

The best method to use for carbon coating is an on going debate. Best to find a method that works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate
  • Flat vs. pointed rod debate

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

View topic  |  History: r11 < r10 < r9 < r8  |  More topic actions...
 
Copyright © by the contributing authors. All material on this collaboration platform is the property of the contributing authors.
Ideas, requests, problems regarding this intranet, Send feedback