Difference: CarbonCoating (10 vs. 11)

Revision 1118 Sep 2009 - Main.KdDerr

 
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Carbon Coating

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>		Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM
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Principles & Protocols

Principles - Carbon Coating

Jump to Protocol

 The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.

Substrates

  • Pure carbon
  • Nitrocellulose
  • Formvar

Deposition Methods

Carbon Thread

  • Reproducible results
  • Varying thread thicknesses available

Carbon Rod

  • Results not 100% reproducible
  • Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
  • Flat vs. pointed rod debate
  • carbon rod sharpening angles:
    carbpts.jpg

  • Set ALLOWTOPICVIEW =

-- KdDerr - 29 Aug 2007

META FILEATTACHMENT attachment="carbpts.jpg" attr="h" comment="carbon rod sharpening angles" date="1188596564" name="carbpts.jpg" path="carbpts.jpg" size="7139" stream="carbpts.jpg" user="Main.KdDerr" version="0"
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