Difference: CarbonRod (1 vs. 9)

Revision 921 Sep 2009 - Main.KdDerr

   META TOPICPARENT   name="VacuumEvaporator"
-  META TOPICPARENT
+ name="VacuumEvaporator"
-<
<
+Using Carbon Rod in Edwards 306
->
>
+Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM
->
>
+ Principles & Protocols 


 Protocols - Using Carbon Rod in Edwards 306 
 Jump to Principles
 THIS PROTOCOL IS FOR PURE CARBON IF USING GRAPHITE DO NOT APPLY AS MUCH JUICE


How to tell the difference:  Graphite can be written with carbon cannot
 

 Insert fresh carbon rod in carbon evaporator source
  Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
  Move stop over sample to prevent C from coating during off gassing
  Lower bell jar 
  Close safety latch on bell jar 
  Push "cycle" wait for bell jar to pump down to 10-4
  Turn dial to #0 (L)
  Switch LT selector to A (10V)
  Switch on LT
  Turn the selector up to # 4 to off gas contamination slowly
  Move sample stop away from sample
  Make sure variable control is at 0
  Add liquid nitrogen to the LN2 trap
  Allow vacuum to recover to 5x10-6 - may need to be restarted 
  Turn variable switch slowly until a glow is present
  Hold for 30 seconds to condition carbon tip
  Turn up variable control slowly to around #6, if sparks are seen back down on the power
  Coat to desire thickness
  Turn down variable control
  Turn selector switch to "0"
  Press "vent"
 





Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup

Revision 831 Aug 2007 - Main.KdDerr

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

THIS PROTOCOL IS FOR PURE CARBON IF USING GRAPHITE DO NOT APPLY AS MUCH JUICE

Added:

>
>

How to tell the difference: Graphite can be written with carbon cannot

Insert fresh carbon rod in carbon evaporator source
Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
Move stop over sample to prevent C from coating during off gassing
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down to 10-4
Turn dial to #0 (L)
Switch LT selector to A (10V)
Switch on LT
Turn the selector up to # 4 to off gas contamination slowly
Move sample stop away from sample
Make sure variable control is at 0
Add liquid nitrogen to the LN2 trap
Allow vacuum to recover to 5x10-6 - may need to be restarted
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip
Turn up variable control slowly to around #6, if sparks are seen back down on the power
Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 731 Aug 2007 - Main.KdDerr

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

THIS PROTOCOL IS FOR PURE CARBON IF USING GRAPHITE DO NOT APPLY AS MUCH JUICE

Added:

>
>

How to tell the difference: Graphite can be written with carbon cannot

Insert fresh carbon rod in carbon evaporator source
Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
Move stop over sample to prevent C from coating during off gassing
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down to 10-4
Turn dial to #0 (L)
Switch LT selector to A (10V)
Switch on LT
Turn the selector up to # 4 to off gas contamination slowly
Move sample stop away from sample
Make sure variable control is at 0
Add liquid nitrogen to the LN2 trap
Allow vacuum to recover to 5x10-6 - may need to be restarted
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip
Turn up variable control slowly to around #6, if sparks are seen back down on the power
Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 631 Aug 2007 - Main.KdDerr

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

Changed:

<
<

THIS PROTOCOL IS FOR PURE CARBON IF USING DO NOT APPLY AS MUCH JUICE

>
>

THIS PROTOCOL IS FOR PURE CARBON IF USING GRAPHITE DO NOT APPLY AS MUCH JUICE

Insert fresh carbon rod in carbon evaporator source
Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
Move stop over sample to prevent C from coating during off gassing
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down to 10-4
Turn dial to #0 (L)
Switch LT selector to A (10V)
Switch on LT
Turn the selector up to # 4 to off gas contamination slowly
Move sample stop away from sample
Make sure variable control is at 0
Add liquid nitrogen to the LN2 trap
Allow vacuum to recover to 5x10-6 - may need to be restarted
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip
Turn up variable control slowly to around #6, if sparks are seen back down on the power
Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 531 Aug 2007 - Main.KdDerr

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

Added:

>
>

THIS PROTOCOL IS FOR PURE CARBON IF USING DO NOT APPLY AS MUCH JUICE

Deleted:

<
<

Insert fresh carbon rod in carbon evaporator source
Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
Move stop over sample to prevent C from coating during off gassing
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down to 10-4
Turn dial to #0 (L)
Switch LT selector to A (10V)
Switch on LT

Changed:

<
<

Turn the selector up to # 2 to off gas contamination slowly

>
>

Turn the selector up to # 4 to off gas contamination slowly

Move sample stop away from sample
Make sure variable control is at 0
Add liquid nitrogen to the LN2 trap
Allow vacuum to recover to 5x10-6 - may need to be restarted
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip

Changed:

<
<

Turn up variable control until sparks are seen

>
>

Turn up variable control slowly to around #6, if sparks are seen back down on the power

Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 431 Aug 2007 - Main.KdDerr

   META TOPICPARENT   name="VacuumEvaporator" 

Using Carbon Rod in Edwards 306
-  META TOPICPARENT
+ name="VacuumEvaporator"
-<
<
+ Place samples on base plate
->
>
-<
<
+ Move stop over samples
  Insert fresh carbon rod in carbon evaporator source
-<
<
+ Insert the carbon evaporator source in vacuum evaporator
->
>
+ Attach carbon evaporator source to rod A&C and grounded rod 14cm from grid
->
>
+ Move stop over sample to prevent C from coating during off gassing
  Lower bell jar 
  Close safety latch on bell jar
-<
<
+ Push "cycle" wait for bell jar to pump down
->
>
+ Push "cycle" wait for bell jar to pump down to 10-4
->
>
+ Turn dial to #0 (L)
  Switch LT selector to A (10V)
  Switch on LT
  Turn the selector up to # 2 to off gas contamination slowly
  Move sample stop away from sample
  Make sure variable control is at 0
-<
<
+ Turn selector switch to "LT"
->
>
+ Add liquid nitrogen to the LN2 trap
->
>
+ Allow vacuum to recover to 5x10-6 - may need to be restarted
  Turn variable switch slowly until a glow is present
  Hold for 30 seconds to condition carbon tip
  Turn up variable control until sparks are seen
  Coat to desire thickness
  Turn down variable control
  Turn selector switch to "0"
  Press "vent"
 





Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup

Revision 313 Jun 2006 - Main.KdDerr

   META TOPICPARENT   name="VacuumEvaporator"
-  META TOPICPARENT
+ name="VacuumEvaporator"
-<
<
+Using Carbon Rod in Edwards 306


 

 Remove glow discharge ring
  Place samples on base plate
  Move stop over samples
  Insert fresh carbon rod in carbon evaporator source
  Insert the carbon evaporator source in vacuum evaporator
  Lower bell jar 
  Close safety latch on bell jar 
  Push "cycle" wait for bell jar to pump down
  Move sample stop away from sample
  Make sure variable control is at 0
  Turn selector switch to "LT" 
  Turn variable switch slowly until a glow is present
  Hold for 30 seconds to condition carbon tip
  Turn up variable control until sparks are seen
  Coat to desire thickness
  Turn down variable control
  Turn selector switch to "0"
  Press "vent"
 





Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005
->
>
+Using Carbon Rod in Edwards 306


 

 Place samples on base plate
  Move stop over samples
  Insert fresh carbon rod in carbon evaporator source
  Insert the carbon evaporator source in vacuum evaporator
  Lower bell jar 
  Close safety latch on bell jar 
  Push "cycle" wait for bell jar to pump down
  Move sample stop away from sample
  Make sure variable control is at 0
  Turn selector switch to "LT" 
  Turn variable switch slowly until a glow is present
  Hold for 30 seconds to condition carbon tip
  Turn up variable control until sparks are seen
  Coat to desire thickness
  Turn down variable control
  Turn selector switch to "0"
  Press "vent"
 





Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005
 

 Set ALLOWTOPICCHANGE = CemfacGroup
-<
<
+ Set ALLOWTOPICCHANGE = CemfacGroup

Revision 222 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

Remove glow discharge ring
Place samples on base plate
Move stop over samples
Insert fresh carbon rod in carbon evaporator source
Insert the carbon evaporator source in vacuum evaporator
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down
Move sample stop away from sample
Make sure variable control is at 0
Turn selector switch to "LT"
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip
Turn up variable control until sparks are seen
Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Added:

>
>

Set ALLOWTOPICCHANGE = CemfacGroup

Revision 118 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="VacuumEvaporator"

Using Carbon Rod in Edwards 306

Remove glow discharge ring
Place samples on base plate
Move stop over samples
Insert fresh carbon rod in carbon evaporator source
Insert the carbon evaporator source in vacuum evaporator
Lower bell jar
Close safety latch on bell jar
Push "cycle" wait for bell jar to pump down
Move sample stop away from sample
Make sure variable control is at 0
Turn selector switch to "LT"
Turn variable switch slowly until a glow is present
Hold for 30 seconds to condition carbon tip
Turn up variable control until sparks are seen
Coat to desire thickness
Turn down variable control
Turn selector switch to "0"
Press "vent"

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

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