Difference: CcdImage (1 vs. 2)

Taking CCD Image with EMMENU

• Blank beam
• Click to expose in Tecnai interface
• Lift screen
• Switch toggle on box from TEM to CCD (right of column)
  • ensure 15 sec for charge to drain off CCD
• Unblank beam
• Click expose in EM Menu (half way down left column)
• Advance image number if you want to save
  • or change settings for autoadvance
• Click power for Fourier Transform

EM Menu saves up to 16 images in memory until explicitly saved on disk

• To save series
  • Under misc menu (bottom right)
  • Adjust numbers to in bottom right dialog box to reflect number of images to save
  • Image format
    • Save as 8 bit TIFF for easy viewing
    • save as a Teitz EM format if planning on image processing
  • Select save directory on TEM data computer
    • mounted as P: drive from control computer
  • Click save series

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

Taking CCD Image with EMMENU

• Blank beam
• Click to expose in Tecnai interface
• Lift screen
• Switch toggle on box from TEM to CCD (right of column)
  • ensure 15 sec for charge to drain off CCD
• Unblank beam
• Click expose in EM Menu (half way down left column)
• Advance image number if you want to save
  • or change settings for autoadvance
• Click power for Fourier Transform

EM Menu saves up to 16 images in memory until explicitly saved on disk

• To save series
  • Under misc menu (bottom right)
  • Adjust numbers to in bottom right dialog box to reflect number of images to save
  • Image format
    • Save as 8 bit TIFF for easy viewing
    • save as a Teitz EM format if planning on image processing
  • Select save directory on TEM data computer
    • mounted as P: drive from control computer
  • Click save series

Visit the Cryo EM Website at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005