Difference: CemAboutWeb (3 vs. 4)
Revision 419 Jun 2012 - Main.EdEng
Cryoelectron Microscopy (CEM) uses electrons accelerated to almost the speed of light to reveal images of individual molecules and molecular assemblies. The electrons illuminate the sample much as light does in an ordinary microscope, but the electromagnetic lenses magnify the image up to one million fold. CEM is highly complementary to NMR and X-Ray Crystallography as it can create images of transient, large, multimolecular complexes not amenable to those technologies. Under the direction of Dr. David Stokes, NYSBC operates one of the most advanced CEM facilities in the U.S. including one 300 kilovolt (kV) microscope, two 200 kV microscopes, and one 120 kV microscope, all with field emission guns, and a Dual-Beam Scanning Electron Microscope, to produce 3D reconstructions of tissue from 1-10 microns. The expert staff at the NYSBC assists in applications of a wide variety of techniques including: tomography, two-dimensional crystallography, single-particle reconstruction, focused ion beam milling and ion abrasion SEM, and correlative microscopy. Additional Structural Biology and EM resources. Twiki Source. |
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