Projects on the Nanolab

Characterization of organelles in identified cells in the nematode, C. elegans

• PI: Dave Hall
• Institution: Albert Einstein College of Medicine
• Researcher: Ken Nguyen
• Brief description: We hope to use electron tomography to extend our reach to recognize finer details of ultrastructure in any cell type. We have begun principally with wild type adult hermaphrodites, while we determine the parameters for best fixation, staining, and sectioning. As we progress in identifying organelles of interest, we will try to characterize the fine details of interesting organelles in several cell types, and then explore for possible changes in their structural organization in selected mutants that are known to affect a specific cell type.

Imaging Session

• date 5/23/2012
• sample C him5L1_052312

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EM- HIV, Dendritic Cells and T Cells

• PI: Dan Littman
• Institution: NYU
• Researcher: Michael Menager
• Brief Description: Use EM to analyze morphology of cells and viruses. How is the morphology of dendritic and/or t cells affected by presence of the virus?

Imaging Session

• date 09/14/12

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Filamentous Bacteria in Mouse Intestine

• PI: Dan Littman
• Institution: NYU
• Researcher: Joshua / Kristin Dancel
• Brief Description: Concentrating on ultrastructure analysis of filamentous bacteria in mouse intestine. Use tomography to monitor / analyze cytoskeleton network changes after filamentous bacteria enters mouse intestine.

Imaging Session

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Synapse morphology in the rat amygdala

• PI Joseph LeDoux
• Institution: NYU
• Researcher: Linnaea Ostroff
• Brief Description: Use large scale tomographic imaging to study synapse morphology in rat amygdala

Imaging Session

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3D mitochondria reconstruction in adrenoleukodystrophy mouse models

• PI Aurora Pujol
• Institution: NYU
• Researcher: Aurora Pujol
• Brief Description: Adrenoleukodystrophy is a rare neurodegenerative disorder affecting brain and spinal cords, characterized by accumulation of very-long chain fatty acids and oxidative stress. We would like to measure mitochondria morphological parameters, such as mitochondrial mass, size and number of cristae of X-Ald mice compared to controls.

Imaging Session

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Quantification of the total number of synapses in different sub-compartments of layer 4C of macaque primary visual cortex

• PI: Michael J. Hawken
• Institution: NYU
• Researcher: Virginia Garcia Marin
• Brief Description: An accurate determination of the total number of synapses in layer 4C of macaque is essential to estimate the proportion of dLGN synapses in the monkey, as layer 4C is the principal recipient layer of thalamic afferent in the visual cortex. The aim of this work is to quantify the total number of synapses into the different compartments of layer 4C of macaque primary visual cortex.

Imaging Session

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Interaction of macrophages with agLDL

• PI: Fred Maxfield
• Institution: Albert Einstein College of Medicine
• Researcher: Lee Cohen-Gould
• Brief Description: We would like to do EM tomography of macrophages interacting with aggregates of LDL. It would be very useful to obtain images of semi-thick specimens (200-300 nm) because we are looking at compartments that may have dimensions of a few hundred nm.

Imaging Session

T cells

• PI: Mike Dustin
• Institution: NYU
• Researcher: Jaime Llodra
• Brief Desription:

Imaging Session

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Structural studies of microsporidi

• PI:
• Institution: AECOM
• Researcher: Peter Takvorian
• Brief Description: The microsporidia are a group of obligate intracellular parasitic protists that have been implicated as both human and veterinary pathogens. We are trying to study these through tomography via FIB/SEM.

Imaging Session

• representative image:
• Set ALLOWTOPICVIEW =

-- BillRice - 22 Oct 2012

* laeana:

* intestine:

* aurora:

* vgarcia 25%:

* c elegans1: