Difference: CemMarisa (1 vs. 2)

Revision 207 Aug 2013 - Main.NicolasCoudray

 Contents

20130807

ArgC? Nanodisc staining.

  • 1/10 dilution of stock in TBS.

  • 20 sec air glow discharge of continuous carbon on copper grids.

  • 4 uL incubated on grid for 30 sec

  • 1 wash in buffer, 1 wash in water, 1 wash in stain

  • 30 sec stain

  • Blotted dry on Whatman 1

Imaged at 50kX on F20.

  • Set ALLOWTOPICVIEW =

* 20130807.zip: images

META FILEATTACHMENT attachment="20130807.zip" attr="" comment="images" date="1375887854" name="20130807.zip" path="20130807.zip" size="78864117" stream="20130807.zip" user="Main.BillRice" version="1"
Added:
>
>
META FILEATTACHMENT attachment="jeol1230_20130807-nanodiscs.zip" attr="" comment="" date="1375890419" name="jeol1230_20130807-nanodiscs.zip" path="jeol1230_20130807-nanodiscs.zip" size="7711873" stream="jeol1230_20130807-nanodiscs.zip" user="Main.NicolasCoudray" version="1"
 

Revision 107 Aug 2013 - Main.BillRice

 Contents

20130807

ArgC? Nanodisc staining.

  • 1/10 dilution of stock in TBS.

  • 20 sec air glow discharge of continuous carbon on copper grids.

  • 4 uL incubated on grid for 30 sec

  • 1 wash in buffer, 1 wash in water, 1 wash in stain

  • 30 sec stain

  • Blotted dry on Whatman 1

Imaged at 50kX on F20.

  • Set ALLOWTOPICVIEW =

* 20130807.zip: images

META FILEATTACHMENT attachment="20130807.zip" attr="" comment="images" date="1375887854" name="20130807.zip" path="20130807.zip" size="78864117" stream="20130807.zip" user="Main.BillRice" version="1"
View topic  |  History: r2 < r1  |  More topic actions...
 
Copyright © by the contributing authors. All material on this collaboration platform is the property of the contributing authors.
Ideas, requests, problems regarding this intranet, Send feedback