Principles and Protocols for EM structural studies

Electron microscopy techniques

• Structural biology using Transmission Electron Microscopy (TEM)
• Electron crystallography
• Single particle analysis
• Electron tomography

Sample preparation techniques

• Grid preparation
• Staining
• Plunge freezing
• High pressure freezing
• Freeze Substitution

Data processing

• Image processing overview
• Electron crystallography
• Helical reconstruction
• Single particle analysis
• Electron tomography

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