Difference: CemSeminarsCoursesArchive (9 vs. 10)
Revision 1004 Oct 2013 - Main.EdEng
(Archive)Courses and Seminars related to CryoEM | ||||||||
| Added: | ||||||||
| > > |
2013At the NYSBCSemester-long Course: "Cryoelectron Microscopy of Macromolecular Assemblies"This comprehensive course will cover the theory and practice of solving molecular structures by electron microscopy. We will start with optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis, and 2D crystallography. We will end with a discussion of map interpretation and molecular fitting. Two weeks are devoted to practical sessions giving students hands-on experience with a variety of software packages for image reconstruction. Offered every fall from September - December. 2013 Course Announcement, view flier, download flierEM Journal Club (If you are interested in presenting a paper please email cem@nysbc.org)Past Journal Club ArticlesSpecial seminarTime: October 4, 2013, 4:00 pm Location: NYSBC, room A-11. Speaker: Manfred Auer, Ph.D. - Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA A Tale of Two Communities: Electron Microscopy 3D Imaging Across the Scales: Life is organized at various scales from macromolecular machines residing in their native cell and tissue context, over organelles and subcellular regions to the interactions of cells and tissues. Imaging biological function across the scales requires the integration of multiple imaging approaches, and requires sophisticated sample preservation (through high pressure freezing), EM-compatible tag-based labeling approaches, fluorescence and EM imaging (2D wide-field TEM and 3D TEM and Focused Ion Beam and Serial Block Face SEM), as well as advances in image 3D visualization, segmentation and quantitative analysis (including CAD model-building and simulations). Using a few selected examples from our ongoing research portfolio, for the world of eukaryotes, I will show examples at the organelle and subcellular volume imaging by electron tomography (plant cell wall and hair cell stereocilia), as well as the cellular and tissue level (mammary gland branching morphogenesis and breast cancer progression model in 3D cell culture ). For the world of prokaryotes, I will show that bacterial biofilms display remarkable 2D and 3D organization at the ultrastructural level that underlie physiological microbial community function. I will end on what happens when two bacterial species encounter one another, and we have just begun to elucidate what strategies these adversaries deploy to ensure community survival.Local Cryo-EM Workshops and MeetingsMinisymposium on Computational Methods in Three-Dimensional Microscopy ReconstructionColumbia University Medical Center [exact location TBA] August 2, 2013 Program Program Chairs: Gabor Herman (CUNY Graduate Center) and Joachim Frank (Columbia University) * Peter Doerschuk (Cornell) Reconstructing heterogeneous particles by estimating the statistics of Fourier series coefficients * Yaser Hashem (Columbia) Challenge posed by heterogeneity in cryo-EM of macromolecules, as exemplified with the eukaryotic pre-initiation complex * Amit Singer (Princeton) Covariance matrix estimation of heterogeneous 3D volumes from their 2D projections// * Yoel Shkolnisky (Tel Aviv University) Robust angular reconstitution in the presence cyclic symmetry * Hemant D. Tagare (Yale) Local resolution, image models, and hypothesis testing * Jose-Maria Carazo (CNB, Madrid) The use of spiral phase transform in
National & International Cryo-EM Meetings, Workshops and Practical CoursesUBC 3D Correlative Light and Electron Microscopy courseUBC Bioimaging FacilityUBC Centre for Highthroughput Phenogenomics This course is designed for life science researchers/microscopists who are looking to learn 3D Correlative Microscopy. Our instructors will guide you with a series of lectures and practical demonstrations. We will introduce a sample (or two) at the beginning of the course and take that one sample through microCT, confocal/2P, dual beam FESEM and TEM tomography and into advanced 3D image processing. Sample preparation will include both chemical fixation as well as high pressure freezing. Synopsis:
For more information contact Garnet Martens at garnet.martens@botany.ubc.ca. http://www.bioimaging.ubc.ca/preliminary-programme-for-the-inaugral-ubc-3d-clem-course-june-14-17-2013/ Penn State College of Medicine Imaging Symposium:The symposium is free to attend but registration is kindly requested: Register for the Imaging Symposium Here <http://forms.pennstatehershey.org/playerf.htm?f=8cpCAgsI> More information: http://www.pennstatehershey.org/web/core/microscopy-histology Penn State College of Medicine Imaging Symposium Wednesday, April 10, 2013 Penn State Hershey University Conference Center Agenda 8:00 – 8:30 a.m. Registration and Continental Breakfast 8:30 – 8:45 a.m. Welcome and Introductions Keynote 8:45 – 9:45 a.m. “Visualizing the life cycle of Dengue virus with electron microscopy” Michael G. Rossmann, Ph.D. Hanley Distinguished Professor of Biological Sciences Department of Biological Sciences Purdue University 9:45 – 10:15 a.m. “Sensing a REAL virus – Trafficking of Poxviral DNA and the Innate Immune Sensor TLR9" Christopher Norbury, Ph.D. Professor, Microbiology and Immunology, Penn State College of Medicine 10:15 – 10:30 a.m. Break 10:30 – 11:00 a.m. “A picture is worth a thousand blots: Using imaging to study late steps in retroviral replication" Darrin Bann, B.A. M.D./Ph.D. Candidate, Penn State College of Medicine 10:00 – 11:30 a.m. “To Be Announced” Keith Cheng, M.D./Ph.D. Chief, Division of Experimental Pathology, Penn State Hershey 11:30 – 12:00 p.m. “Localization of Myosin III in Photoreceptors: Conservation from Drosophila to Humans” Christopher Yengo, Ph.D. Associate Professor of Cellular and Molecular Physiology, Penn State College of Medicine 2:00 – 1:30 p.m. Lunch & Posters Keynote 1:30 – 2:30 p.m. “The biogenesis of HIV-1: lessons from imaging the assembly one virus at a time” Sanford M. Simon, Ph.D. Professor, Head, Laboratory of Cellular Biophysics The Rockefeller University, New York 2:30 – 3:00 p.m. "How do multiphoton and harmonic generation microscopy methods enable biologists to understand complex cellular processes" Thomas Abraham, Ph.D. Director, Imaging Core, Penn State College of Medicine Keynote 3:00 – 4:00 p.m. “High resolution cryo-electron microscopy applied to the icosahedral capsids of tailed bacteriophages and herpesviruses” James F. Conway, Ph.D. Associate Professor Department of Structural Biology University of Pittsburgh School of Medicine2nd International caesar Conference “From molecules to cells – New frontiers in 3D electron microscopy”21 to 24 May, 2013 in Bonn. Presentations will be given by invited speakers and speakers chosen from submitted abstracts to highlight work of young investigators and late-breaking developments. The program includes poster presentations and social events, including a welcome reception and a farewell party. For young researchers, a workshop on "Cryo-electron tomography of vitreous sections" is held prior to the Conference (May 20 to 21, 2013). Details and registration procedure are given on: http://www.caesar.de/conference2013.html For any additional information or assistance, please contact Brigitte Bauer Team Assistant phone +492289656-267the 3rd UB Practical Course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS, a.k.a cryoEM of frozen-hydrated sections)2-4 July 2013 / 9-11 July 2013 Organiser : Benoît Zuber Instructors: Benoît Zuber, Daniel Studer, Ioan Iacovache The objective of the course is to teach participants the practical skills necessary to successfully apply CEMOVIS (a.k.a. Frozen-hydrated sections) in their laboratories. Our latest tricks making CEMOVIS quite easy will be demonstrated. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging of vitreous sections. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. The 3-day course is given twice for two different groups of maximum 4 participants each so that every participant can be actively practicing during the whole course. All the participants of the previous previous courses have been successful in producing, collecting and imaging vitreous sections. The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite. Information and registration: http://www.ana.unibe.ch/events/cemovis/index.html Registration deadline: 17.03.2013International Workshop of Advanced Image Processing of Cryo-Electron Microscopy (IWAIP-CryoEM)10th to 13th July, 2013 in Beijing. During the workshop, in the morning sessions, lectures will be given by invited speakers highlighting the frontiers of image processing on single particle analysis. After the morning lectures, practice courses are arranged in the afternoon, followed by evening discussion of the in-depth application of software packages. The program includes social events, such as the welcome reception, travel awards and a farewell party. The practice courses in the afternoon session during the workshop will focus on tutorials of image processing packages including EMAN2, XMIPP3.0 and RELION. Details and registration procedure are given on the website http://cbi.ibp.ac.cn/workshop/ For any additional information or assistance, please contact Dr. Yan Zhang Team Assistant Phone +86-10-64888137 Fax +86-10-64888376
The Sixth Brazil School for Single Particle Cryo-EM(14 - 26 August 2014)This single-particle data processing school is an intensive hands-on course/workshop, in which theory and practice are tightly intertwined.The spectrum of topics covered suits attendees who are new to the field as well as those for whom the technique is already of growing importance in their research and wish to acquire an in-depth understanding of the methodology. Topics range from data collection and three-dimensional reconstruction techniques to the final interpretation of the cryo-EM maps by (flexible) fitting of X-ray structures. The last days of the meeting will be devoted to advanced topics such as dealing with heterogeneous data sets ("4D" cryo electron microscopy) and high-resolution refinements. The attendees are expected to bring along a modern notebook computer onto which all necessary software will be installed. Most processing will take place in the context of the IMAGIC 4D software system but other software packages such as Frealign will also be used. Cryo-EM data sets will be distributed for processing during the course. The participants may bring their own data sets for processing and discussion. For the latest information: www.single-particles.org/school/EMBO practical course on image processing for cryo-electron microscopy in BirkbeckCollege , London . This 10 day course will run from 3 September ˆ 13 September 2013. http://www.ismb.lon.ac.uk/embocourse.html The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists wishing to determine macromolecular structures by cryo electron microscopy (EM). The course will concentrate on processing of single particle images, and will be aimed at advanced PhD students and postdocs working on projects producing cryo EM images for structural analysis. Familiarity with UNIX would be an advantage. Morning sessions will include three 50-minute lectures, breaks and a discussion period. A 3-hour practical would take place in the afternoon, with discussion time at the end. After dinner there will be research seminars or student presentations. Applications are invited from PhD students and researchers in EM. See details on http://www.bbk.ac.uk/biology/our-research/embo-practical-course Applications are invited from PhD students and researchers in EM. * *Applications should include: a Curriculum vitae; a publication list; a short description of your current work and future plans; an explanation of why attendance at the course would further the applicant's own research. A letter of recommendation from the applicant's supervisor (to be sent by the supervisor)** Deadline for applications: Monday 20 May 2013 | |||||||
2012At the NYSBCSpecial Seminar AnnouncementSmoking guns and dancing couples: Molecular Machines at the Membrane(Cryo‐EM studies of the Type 3 secretion complex) Dr. Thomas Marlovits Research Group Leader Research Institute of Molecular Pathology, Research Institute of Medical Biotechnology Austrian Academy of Sciences 2:00 p.m. July 30, 2012 Conference Room A‐11 New York Structural Biology Center 89 Convent Avenue at 133rd St. ADVANCES IN FLUORESCENCE MICROSCOPY OF THE CELLPlease join us for a second single-molecule workshop at the New York Structural Biology Center. When:Wednesday, May 9, 2012 from 9:30 a.m. - 2:30 p.m. Where: New York Structural Biology Center 133rd Street & Convent Avenue Keynote Speaker: Eric Betzig on Bessel beam plane illumination microscopy. Scientist, HHMI Janelia Farm Other Speakers: Rob Singer, Albert Einstein College of Medicine David Spector, Cold Spring Harbor Laboratory Ben Ovryn, Albert Einstein College of Medicine Mike Dustin, NYU School of Medicine Ben Chen, Mount Sinai School of Medicine -LUNCH WILL BE SERVED- POSTER SESSION RSVP: 509WORKSHOP@NYSBC.ORG Please note that due to limited space, we ask that you limit attendance to no more than 2 people from each lab. Directions: http://www.nysbc.org/AboutUs/directions.html Workshop in Dual-Beam Scanning Electron Microscopy at the New York Structural Biology CenterThis workshop will introduce the new Helios 650 Nanolab microscope at NYSBC and illustrate its capabilities for producing large scale (10um x 10um x 10um) 3D reconstructions through resin-embedded tissue as well as for preparing frozen, whole-mount cells for electron tomography. In the literature, this method is alternately referred to as Focused Ion Beam (FIB) milling and Ion Abrasion SEM.
Local Cryo-EM Workshops and MeetingsNew York Structural Biology Group 2012 Summer MeetingThis is the 67th meeting of the Group. Thursday, August 9th, 2012, 9:30-5:50 Weill Cornell University Medical College, Uris Auditorium 69th St and York Avenue, NY, NY This all-day meeting will include presentations by local researchers, lunch, a poster session, and post-meeting reception. Coffee and refreshments served from 900 am. There will again be a POSTER COMPETITION with cash prizes for the winners! You must register your poster in advance because of space limitations, there will be selection of posters to provide representation of labs, institutions, and research areas. For details and registration please visit http://www.nysbdg.org/Minisymposium on Computational Methods for Three-Dimensional Microscopy ReconstructionThe Science Center (Room 4102) The Graduate Center of the City University of New York 365 Fifth Avenue (corner of 34th St) New York, NY 10016 June 15, 2012 Program Program Chairs: Gabor Herman (CUNY Graduate Center) Joachim Frank (Columbia University)
National & International Cryo-EM Meetings, Workshops and Practical CoursesEMBO practical course on cryo-EM and 3D image processingThe EMBO practical course on cryo-EM and 3D image processing will take place this year from 26th August-2nd September at EMBL in Heidelberg. As in the past, the course will provide in depth theoretical and practical training, with the focus on image processing for 3D reconstruction. Registration is now open. Further details, including a list of confirmed teachers are available here: http://www.embl.de/training/events/2012/CRY12-01/index.htmlWorkshop on Image Processing with 2dxAugust 19-25, 2012, in Basel, Switzerland. We invite for applications to participate in the fourth international workshop on electron crystallography of membrane proteins. This workshop will focus on data processing, with emphasis on 3D reconstructions from images of tilted 2D crystals. The hand-on workshop on the processing of images from cryo-electron microscopy of 2D crystals of membrane proteins (electron crystallography) will provide the theoretical background and practical applications in advanced image processing algorithms. The workshop will use the 2dx software system, which is based on the MRC programs. Theoretical morning lectures will be followed by practical exercises in the afternoon. Although we will provide iMacs for every participant, you are encouraged to bring your own laptop (Linux or OSX), or at least a portable hard-drive (USB2.0), onto which we will install the relevant software systems and test data during the workshop. You are also welcome to bring your own dataset. Speakers and instructors include: Marcel Arheit, C-CINA, Biozentrum, University of Basel, Switzerland. Daniel Castano-Diez, C-CINA, Biozentrum, University of Basel, Switzerland Anchi Cheng, Scripps, CA, USA. Hans Hebert, Karolinska, Stockholm, Sweden. Cristina Paulino, MPI for Biophysics, Frankfurt, Germany. Sebastian Scherer, C-CINA, Biozentrum, University of Basel, Switzerland Henning Stahlberg, C-CINA, Biozentrum, University of Basel, Switzerland. Xiangyan Zeng, FVSU, GA, USA The detailed program is available here: http://www.2dx.unibas.ch/workshop/2012 A registration fee of 300 CHF (approx. $340) for participants from non-profit organizations includes lunch and snacks. Registration is open here: http://www.2dx.unibas.ch/workshop/2012/registration-for-the-workshop Oganizers: Marcel Arheit, Karen Bergmann, and Henning Stahlberg.The Fifth Brazil School for Single Particle Cryo-EM (9-21 August 2012)This single-particle data processing school is an intensive hands-on course/workshop, in which theory and practice are tightly intertwined. The spectrum of topics covered suits attendees who are entirely new to the field as well as those who wish to acquire an in-depth understanding of the methodology. Topics range from data collection and three-dimensional reconstruction techniques to the final interpretation of the cryo-EM maps by fitting X-ray structures. The last days of the meeting will be devoted to advanced topics such as dealing with heterogeneous data sets ("4D" cryo electron microscopy) and high-resolution refinements. A special emphasis will be placed this time on the processing of massive data sets collected automatically with the newest generation of microscopes (KRIOS). The attendees are expected to bring along a modern notebook computer onto which all necessary software will be installed. Most processing will take place in the context of the IMAGIC software system but other software packages such as Frealign will be used. Cryo-EM data sets will be distributed for processing during the course. The participants may bring their own data sets for processing and discussion. The meeting will take place in the Grinberg's Village Hotel, which is situated in the scenic countryside of Socorro, about 2 hours drive from Sao Paulo. For more information and registration see: www.single-particles.org/school/2nd UB Practical course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS)5-7 June 2012 / 12-14 June 2012 Institute of Anatomy, University of Bern, Switzerland Organiser : Benoit Zuber Instructors: Benoit Zuber, Daniel Studer, Dimitri Vanhecke The objective of the course is to teach the participants the practical skills necessary to successfully apply CEMOVIS in their laboratories. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. The 3-day course is given twice, each time with a maximum of 4 participants so that they can be actively practicing at any time. The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite. For details and registration please visit http://www.ana.unibe.ch/event/cemovis/EMBO practical course on Electron Tomography in Life ScienceRegistration is now open for the EMBO practical course on Electron Tomography in Life Science 18-23 June, 2012, Leiden, The Netherlands See https://electronmicroscopy.lumc.nl/embo2012.html for registration and a detailed description of the course. Application deadline is March 31st 2012Electron Tomography of Macromolecular Complexes and Cells with SerialEM (online seminar)Offered in collaboration with the National Center For Macromolecular Imaging. The schedule is designed to give lots of time for people to ask questions. It is set up with somewhat independent segments that have half-hours breaks between them, so you can participate in whichever segments fit your time and interest. (Schedule is in Central Standard Time)Day 1 (Sep 27, 2012: Thursday)
Day 2 (Sep 28, 2012: Friday)
A Workshop on Advanced Topics in EM Structure DeterminationThe National Resource for Automated Molecular Microscopy Announces A Workshop on Advanced Topics in EM Structure Determination November 11-16, 2012 National Resource for Automated Molecular Microscopy The Scripps Research Institute (TSRI), La Jolla, California. We invite applications to participate in a Workshop on EM Structure Determination of Challenging Macromolecules. The workshop is aimed at students, postdocs and faculty that already have some experience practicing cryoEM. We can accommodate a total of 100 participants. A major goal of the workshop will be to discuss some of the challenges that we face in using cryoEM to solve the structure of macromolecules. Topics to be discussed will include improvements in specimen preparation, imaging, processing and reconstruction, and methods for validation. The basic format of the course will be theoretical lectures in the mornings followed by practical demonstrations and panel discussions in the afternoon. Evenings will be reserved for poster presentations by participants and research talks by instructors. The instructors for the course will include: Yifan Cheng (UCSF), Wah Chiu (Baylor), David DeRosier (Brandeis), Joachim Frank (Columbia), Bob Glaeser (LBL), Niko Grigorieff (Brandeis), Richard Henderson (MRC), Tilak Jain (TSRI), Gabe Lander (Berkeley), Steve Ludtke (Baylor), Pawel Penczek (U. Texas- Houston), John Rubinstein (Hospital for Sick Children, Toronto), Sjors Scheres (MRC), Holger Stark (Max Planck- Gottingen), Elizabeth Villa (MPI), and Tom Walz (Harvard). Application forms may be found online at the workshop web page: http://nramm.scripps.edu/?page_id=2424 A registration fee of $750 will be charged to participants from non-profit institutions. Some partial scholarship support may be available depending on the success of efforts to raise support. All meals will be provided as part of the workshop registration fee but participants will be expected to cover their own travel and lodging expenses. Cost of housing is estimated to be under $100/night for a shared room and transportation will be provided between the hotel and the workshop venue. Further details on these and other matters will be provided once the registration process begins. The application deadline is 1 July and a final selection of participants will be made by 15 August. Please see all other information pertaining to the course at the web site: http://nramm.scripps.edu/?page_id=2424 For other inquiries send email to: nramm@scripps.edu Organizers Bridget Carragher, Clinton S. Potter and Ronald A. Milligan National Resource for Automated Molecular Microscopy, The Scripps Research Institute
| ||||||||
View topic
| History: r10 < r9 < r8 < r7
| More topic actions...
Copyright © by the contributing authors. All material on this collaboration platform is the property of the contributing authors.
Ideas, requests, problems regarding this intranet, Send feedback
Ideas, requests, problems regarding this intranet, Send feedback