Difference: ColdStage ( vs. 1)

Cold Stages

Gatan

• This holder easily can obtain 1nm resolution

Oxford (Dual Tilit High Resolution Holder)

• This holder is not necessary if area of interest is greater than 6 Angstroms
• When filling dewar with liquid nitrogen make sure the top plate does not get frozen

Gatan Dry pumping station

• Insert specimen rod into the pumping station
  • Open "V1" and "V2"
  • Make sure the screw valve on tee is closed
  • Turn on pump

Warm up

• Invert the dewar during warm up to facilitate expulsion of condensation
  • Pump on the specimen rod only during warm-up.
• Attach the plug on the back of the "Gatan Cold Stage Control" to the dewar
• Turn on "Gatan Cold Stage Control"
  • Switch is in back of box
• Turn knob until "Start Warm-up Cycle" is selected
  • Press any one of the three buttons to select
  • Press far left button to begin
  • This cycle will take around 30 min. You must wait for the cycle to finish before pumping on the dewar. This prevents contamination on the Zeolite in the dewar; one of the causes of instability.

Room Temperature

• Close the "V1" valve
• Return dewar to the upright position
• Connect tygon hose from tee to dewar
• Unscrew valve at end of tee
  • Allow the tygon tube to evacuate for a minute before opeing dewar
  • This is to prevent contamination of the dewar's zeolite
• Unscrew the vavle on the dewar
• leave the stage pumping overnight

• Set ALLOWTOPICVIEW =

-- KdDerr - 30 Oct 2007