Difference: DongyanTan (2 vs. 3)

• Publication: Tan D., Rice W.J., Sosa H. Structure of the Kinesin13 Ring Complex. Structure 2008 Nov 12; 16 :1732 1739

Toinvestigate the mechanismof kinesin13-inducedmicrotubule depolymerization, we have calculated a three-dimensional (3D)mapof the kinesin13-microtubule ring complex, using cryo-electron microscopy (cryo-EM) and image analysis. An atomic model of the complex was produced by docking the crystal structures of tubulin and a kinesin13 motor domain (MD) into the 3D map. The model reveals a snapshot of the depolymerization mechanism by providing a 3D view of the complex formed between the kinesin13 MD and a curved tubulin protofilament (pf). It suggests that contactsmediated by kinesin13 class-specific residues in the putative microtubule-binding site stabilize intra-dimer tubulin curvature. In addition, a tubulinbinding site on the kinesin13 MD was identified. Mutations at this class-conserved site selectively disrupt the formation of microtubule-associated ring complexes.