Difference: F20Checklist (1 vs. 13)

F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)
• 50 micron condenser aperture (check white board, for now it's position #2)

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun

• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)
• 50 micron condenser aperture (check white board, for now it's position #2)
• spot size 2

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun
• Adjust the gun tilt alignment to Trash.findDFdf the brightest spot
• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)

• spot size 2

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun
• Adjust the gun tilt alignment to Trash.findDFdf the brightest spot
• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)
• 50 micron condenser aperture
• spot size 2

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun
• Adjust the gun tilt alignment to Trash.findDFdf the brightest spot
• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)
• 50 micron condenser aperture
• spot size 2

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun

• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger

• • Do not insert holder

• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• insert holder to first stop
  • Do not insert holder
• Uncheck Turbo Auto Off
• Open colum valves
  • check for beam

• Operation Parameters * Arround 3900-3950 extraction voltage * Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.) * 50 micron condenser aperture * spot size 2

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

• Checking Gun Tilit/Brightness
  • Magnification 10kx
  • Check exposure time without the small screen
    • Around 2 sec is appropiate
    • 2 sec exposure means 1 nanoAmp of current

• Condensor Stigmation
  • spot size 2
  • Magnification 250kx
  • condense beam to 30mm size
  • press stigmator button
  • select condensor stigmation
  • use multifunction knobs to make beam round
  • Condense beam to point
    • beam may not be round at crossover, this is the image of the FEG tip
    • 5mm circle = 10nm
    • you want the majority of your beam to fit within the 5mm circle

• Flatfield Tietz camera

• New user Low Dose setup
  • make sure specimen rod is still inserted
    • This will make spot size 2 the default, if rod is out 5 is default.

• • save as new user file and folder

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• insert holder to first stop
  • Do not insert holder
• Uncheck Turbo Auto Off
• Open colum valves
  • check for beam

• Operation Parameters

• Checking Gun Tilit/Brightness
  • Magnification 10kx
  • Check exposure time without the small screen
    • Around 2 sec is appropiate
    • 2 sec exposure means 1 nanoAmp of current

• Condensor Stigmation
  • spot size 2
  • Magnification 250kx
  • condense beam to 30mm size
  • press stigmator button
  • select condensor stigmation
  • use multifunction knobs to make beam round
  • Condense beam to point
    • beam may not be round at crossover, this is the image of the FEG tip
    • 5mm circle = 10nm
    • you want the majority of your beam to fit within the 5mm circle

• New user Low Dose setup
  • make sure specimen rod is still inserted
    • This will make spot size 2 the default, if rod is out 5 is default.

• • save as new user file and folder

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• insert holder to first stop
  • Do not insert holder
• Uncheck Turbo Auto Off
• Open colum valves
  • check for beam

• Operation Parameters * 3950 extraction voltage * Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.) * 50 micron condenser aperture * spot size 2
• Checking Gun Tilit/Brightness
  • Magnification 10kx
  • Check exposure time without the small screen
    • Around 2 sec is appropiate
    • 2 sec exposure means 1 nanoAmp of current

• Condensor Stigmation
  • spot size 2
  • Magnification 250kx
  • condense beam to 30mm size
  • press stigmator button
  • select condensor stigmation
  • use multifunction knobs to make beam round
  • Condense beam to point
    • beam may not be round at crossover, this is the image of the FEG tip
    • 5mm circle = 10nm
    • you want the majority of your beam to fit within the 5mm circle

• New user Low Dose setup
  • make sure specimen rod is still inserted
    • This will make spot size 2 the default, if rod is out 5 is default.
  • Load known good Low Dose file like KD0.lds
  • save as new user file and folder

F20 Daily Checklist

• Cool microscope cold finger
• insert holder to first stop
  • Do not insert holder
• Uncheck Turbo Auto Off
• Open colum valves
  • check for beam

• Operation Parameters * 3950 extraction voltage * Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.) * 50 micron condenser aperture * spot size 2
• Checking Gun Tilit/Brightness
  • Magnification 10kx
  • Check exposure time without the small screen
    • Around 2 sec is appropiate
    • 2 sec exposure means 1 nanoAmp of current

• Condensor Stigmation
  • spot size 2
  • Magnification 250kx
  • condense beam to 30mm size
  • press stigmator button
  • select condensor stigmation
  • use multifunction knobs to make beam round
  • Condense beam to point
    • beam may not be round at crossover, this is the image of the FEG tip
    • 5mm circle = 10nm
    • you want the majority of your beam to fit within the 5mm circle

• Set ALLOWTOPICVIEW =

-- KdDerr - 10 Oct 2006

F20 Daily Checklist

• Cool microscope cold finger
• insert holder to first stop
  • Do not insert holder
• Uncheck Turbo Auto Off
• Open colum valves
  • check for beam

• Operation Parameters

• Checking Gun Tilit/Brightness
  • Magnification 10kx

• • • 2 sec exposure means 1 nanoAmp of current

• Condensor Stigmation

• Set ALLOWTOPICVIEW =

-- KdDerr - 10 Oct 2006

• Set ALLOWTOPICVIEW =

-- KdDerr - 10 Oct 2006

• Operation Parameters o 3950 extraction voltage o Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.) o 50 micron condenser aperture o spot size 2 * Checking Gun Tilit/Brightness o Magnification 10kx + 2 sec exposure means 1 nanoAmp of current

* Condensor Stigmation o spot size 2 o Magnification 250kx o condense beam to 30mm size o press stigmator button o select condensor stigmation o use multifunction knobs to make beam round o Condense beam to point + beam may not be round at crossover, this is the image of the FEG tip + 5mm circle = 10nm + you want hte majority of your beam to fit within the 5mm circle

• Set ALLOWTOPICVIEW =

-- KdDerr - 10 Oct 2006