Difference: FindBeam (1 vs. 8)

Revision 810 Aug 2009 - Main.BillRice

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open
Changed:
<
<

Still cannot Trash.findDFdf beam

>
>

Still cannot find beam

 
  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture

3200 only

  • Make sure energy filter is not inserted

1230 only

  • Make sure camera is not inserted

  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 717 Sep 2008 - Main.DavidCowburn

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open

Still cannot Trash.findDFdf beam

  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture

3200 only

  • Make sure energy filter is not inserted

1230 only

  • Make sure camera is not inserted

  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 616 Jul 2008 - Main.DavidCowburn

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open
Changed:
<
<

Still cannot find beam

>
>

Still cannot Trash.findDFdf beam

 
  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture

3200 only

  • Make sure energy filter is not inserted

1230 only

  • Make sure camera is not inserted

  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 528 Sep 2007 - Main.BillRice

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open

Still cannot find beam

  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture
Changed:
<
<

3200 Only

>
>

3200 only

 
  • Make sure energy filter is not inserted

1230 only

  • Make sure camera is not inserted

  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 428 Sep 2007 - Main.BillRice

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open

Still cannot find beam

  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture
Added:
>
>

3200 Only

  • Make sure energy filter is not inserted

1230 only

  • Make sure camera is not inserted
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 317 May 2007 - Main.KdDerr

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
  • Make sure the colum valves are open

Still cannot find beam

  • Slowly drop the magnification one step at a time
  • Once beam is located
    • Condense beam
    • Center the spot
Added:
>
>

2100 only

  • Press "photo" button
    • microscope may think it is taking a picture
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 213 Sep 2006 - Main.KdDerr

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on
Added:
>
>
  • Make sure the colum valves are open
 

Still cannot find beam

  • Slowly drop the magnification one step at a time
Changed:
<
<
  • Once beam is located, center with brightness centering
>
>
  • Once beam is located
Added:
>
>
    • Condense beam
    • Center the spot
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

Revision 113 Sep 2006 - Main.KdDerr

 
META TOPICPARENT name="CryoEM"
Contents

Instructions for finding the beam

  • Move sample to make sure you are not on a grid bar
  • If you are using the cryostage make sure the shutter is open
  • Make sure High Tension is on

Still cannot find beam

  • Slowly drop the magnification one step at a time
  • Once beam is located, center with brightness centering

  • Set ALLOWTOPICVIEW =

-- KdDerr - 13 Sep 2006

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