Difference: Jeol1230Information (2 vs. 3)

Revision 328 Sep 2007 - Main.BillRice

 
META TOPICPARENT name="CryoEM"
Contents

Microscope Parameters

wavelengths

  • 80kV: 0.04176 A
  • 120kV: 0.03349 A

objective lenses

  • f = ??
  • Cs = ?? mm
  • Chromatic aberration = ?? mm

Aperture Sizes

  • Condensor : 300 um, 200 um, 100 um
  • Objective : 80 um, 50 um, 20 um
  • Selected Area: 300 um, 100 um, 20 um

CCD information

  • Size of camera : 1350 x 1040 pixels
  • Pixel size: 6.45 um
  • Pre-film mag : 0.0617 (!!)
Changed:
<
<
  • Gain uncalibrated
>
>
  • Gain uncalibrated, unlikely to be used for low-dose ccd acquisition
 

Magnification Calibration : Jeol 1230

  • Magnifications calibrated using 2160 cross grid using method described in SerialEM documentation
Film Magnification CCD Magnification Unbinned Pixel Size (A) Rotation (relative to mag 1 step lower)
2,500 153 421 NA
3,000 185 349 0.34
4,000 246 262 0.29
5,000 307 210 -0.05
6,000 368 175 -3.03
8,000 491 131 -6.10
10,000 625 103 -6.27
12,000 757 85.2 6.58
15,000 920 70.1 -0.58
20,000 1,230 53.2 0.64
25,000 1550 41.7 0.00
30,000 1830 35.2 0.41
40,000 2470 26.1 0.43
50,000 3080 20.95 0.41
60,000 3710 17.4 0.21
80,000 4960 13.0 0.41
100,000 6210 10.4 -0.12
120,000 7500 8.60 0.45
150,000 9440 6.83 -0.10
200,000 12,570 5.13 0.56
250,000 14,740 4.38 0.47
300,000 18,140 3.56 -2.11
* Overall CCD to film factor: 0.0617

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-- BillRice - 28 Sep 2007

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