Download the above file and burn it as a CD image. Reboot your computer, choosing this CD as the boot device. This will bring you into KNOPPIX, a "live" linux environment.
The CD should work on just about any Windows PC, notebooks included. It will not work on a PowerPC Mac. Works on an Intel Mac using Parallels.
To open a shell, click on the"Konsole" icon at the bottom of the screen (to the right of the little house)
This CD contains EMAN 1.7 (EMAN, Copyright 1997-2005, Baylor College of Medicine) , Spider 14 (Copyright 1981-2005, Health Research Incorporated (HRI)), and EM2EM (copyright Image Science Software GmbH) . Please do not redistribute this CD
When booting from this CD, the hard drive on your computer is not used. To save files permanently, attach a USB drive and save there. The system should automatically recognize any drive you plug in.
N.B. there are (bad) issues with Linux writing to NTFS disks - don't try it
FAT32 disks are ok
if in doubt, don't try to write to your hard drive using this Knoppix CD - stick with the USB drive
When done, choose to reboot or turn off the system, remove the CD and the computer will start as normal.
Use of EM software contained on Knoppix Live CD
Eman -- started by typing "eman"
Spider -- started by typing "spider"
Web -- does not appear to work in knoppix 24-bit graphical environment, instructions for 8-bit version are as follows:
Changed:
< <
Press CTRL-ALT-F2 -- should see a text screen with prompt "root@tty2#"
> >
Press CTRL-ALT-F2 -- should see a text screen with prompt "root\@tty2#"
type "su - knoppix"
type "/etc/skel/startx8.com" -- an 8-bit graphical display should start up
open a terminal window by right-clicking (menu will come up). Select XShells --> Xterm
start web by typing "web8 [file extension]"
May have to clear screen by choosing Commands --> Clear
Switch back to 24-bit screen by pressing CTRL-ALT-F5.
8-bit screen can be accessed again by pressing CTRL-ALT-F6.
Image data can be introduced through the USB drive discussed above