Difference: LabSafetyEnv (30 vs. 31)

Revision 3112 Jun 2008 - Main.DavidCowburn

 
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Contents --

Laboratory Safety and Environment Issues at NYSBC

Human/ animal samples

ALL human or animal samples must be derived from fully documented procedures which ensure that NYSBC conforms to both general Federal regulations, and to clearly acceptable laboratory safety rules. See Nregim.HumanAnimalOrigin

Human and animal derived samples may be contaminated by accident or design with infectious or toxic agents.

Infectious / toxic agents

All samples should then be treated with extreme care and conform to the Chemical Hygeine Plan and to generally accepted Biosafety rules. The following points should be specifically emphasized
  1. Wear safety glasses, gloves, and disposable lab coat.
  2. Any procedure with the potential to generate aerosols should be written out and reviewed with lab safety staff for conformance
  3. ALL SAMPLES must be returned to the user and then removed from NYSBC, or alternately be put in the Medical Waste stream for disposal.
  4. Any sample storage must conform to a written procedure, and will normally require seperate storage resources SOLELY for human and animal specimens.

Recombinant issues

See http://www.nysbc.net/twiki/bin/viewfile/Main/LabSafetyEnv?rev=1;filename=NIH_Gdlnes_lnk_2002z.pdf or original NIH documents

Aug 5 2005, Biological Inventory

We need to keep a record of all biological inventory of recombinant and other materials for safety reference.

For this purpose, all NYSBC labs and facilities, and all affiliate use must be consisent with,

Changed:
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  1. must certifythat there are no CDC select agents at NYSBC or reagents derived from them.
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  1. must certify that there are no CDC select agents at NYSBC or reagents derived from them.
 
  1. must certify that all recombinant DNA projects fall within the NIH Guildlines expemption. See http://www4.od.nih.gov/oba/guidelines.html
Changed:
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  1. must certify that there is compliance with the OSHA Blood borne pathogen standard.
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  1. must certify that there is compliance with the OSHA Blood borne pathogen standard.
 
  1. must advise NYSBC on the use of any organism for recombinant activity other than non-pathogenic strains of E. coli

As a generic rule, we assume that all NYSBC labs/facilities, and affiliate users will be using non-pathogenic strains of E. coli for protein preparation purposes, that the organism is stored in the general wet labs, and that the scientific director of each NYSBC lab/facility and each affiliate PI is the responsible PI.

Additional Requirements on Affiliates

Changed:
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<		You must have received standard chemical / biochemical safety training in your home institution. You must adhere to all the requlations of your home institution, when at NYSBC, in addition to NYSBC's requirements. If your home institution, for example, insists that all gloves, paper etc go in the biological hazardous waste stream, then do so.
>
>		You must have received standard chemical / biochemical safety training in your home institution. You must adhere to all the regulations of your home institution, when at NYSBC, in addition to NYSBC's requirements. If your home institution, for example, insists that all gloves, paper etc go in the biological hazardous waste stream, then do so.
 

All hours access.

See http://www.nysbc.net/procedures/allhours.htm

Material Handling Sheets in A22 and in each wet lab specifically.

Electronic copies at MaterialDataSheet

Disposal procedures

Glass All glass items or broken glass after any decontamination must be placed in the specific glass disposal containers. NO GLASS MAY BE PUT IN THE WASTE BASKETS, BECAUSE OF HAZARD TO CUSTODIAL PERSONNEL. This applies to glass broken ANYWHERE in the building, not just in labs
Sharps Any needles or similar items must be placed in the sharps' disposal boxes.
Osmium derivatives The CEM Safety Officer will arrange disposal
Chemicals In process of discussion with vendor... ask a Safety Officer
Pump oil , other oils Set aside and store for chem disposal
coli or yeast cultures liquid cultures should be mixed with commercial bleach (90:10 ratio), and flushed down a sink
Petri dishes put in the biological hazardous material waste disposal packs

Note that we are exempt from EPA supervision on the basis of less that 225 lb / period production of hazardous chemicals. Anyone contemplating need for use or disposal of larger quantities MUST GET EXECUTIVE PERMISSION. Note that the period is probably 90 days.

Disposal vendors

This list is of the approved vendors for waste disposal. Current criteria are
  1. CCNY currently a customer, or 2 or more Member Institutions customers
  2. Specific quote on hand for disposal
  3. EPA approval confirmed by NYSBC staff at http://www.epa.gov/opptintr/pcb/stordisp.html
  4. SPECIFIC APPROVAL BY EXECUTIVE
VENDOR Criterion 1 Quote on hand EPA checked Approved
Clean Harbors CCNY NO YES await quote, visited Apr 25 05
Triumvirate Environmental CCNY NO NYD077444263 await quote(s)

Related Documents , copies in A22 or A26.

http://www.nysbc.net/library/LSE/42cfr73.pdf Federal Register Description of Bio toxins and their regulation
http://www.nysbc.net/library/LSE/4th%20BMBL.pdf NIH Safety Guide
OSHA oxygen atmosphere requirement
Chemical Hygeine Plan
http://bmbl.od.nih.gov/sect3bsl1.htm NIH Safety level guidlines

Safety Officers

CEM related activities , Cell Biophy Lab KdDerr
NMR related activities, Phys Biochem Lab KaushikDutta
Beamlines Randy Abramowitz and BNL staff
NYCOMPS at Park Bldg JamesLove

No permission to view HoodProcedures

RadiationExposure

-- DavidCowburn - 24 Nov 2004

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