Low Dose in Serial EM

Setting up Low Dose in Serial EM

• Search mode
  • 10kx center beam FLC off
  • Expand beam
  • Click SA Diff
  • Use PLA to move Beam to camera
  • Drag FLC to top

* Focus Mode

• • 200kx
  • Check additional beam shift box in Serial EM
  • Move beam with image shift
  • Uncheck additional beam shift box when done moving

• Obtaining Live focus mode
  • Drop screen in focus mode
  • Set mag/brightness
  • Check/uncheck continous update of mag and beam
    • This remembers: Mag/birghtness/spotsize
  • Switch to TEM mode
  • Lift screen
  • Click unblank in Serial EM

Using Serial EM in Low Dose mode

• Search Mode
  • Click SA Diff on TEM - it does not remember diffraction
  • Slide FLC
  • Switch to CCD
  • FLC - all off
  • Can take a view image if the other modes are set up
  • If you apply an image shift, click reset image shift to move stage to new position

*Focus Mode

• • Select Focus as area to show when screen down
    • lower/raise screen
  • Switch to TEM
  • Click unblank in Serial EM
  • Focus
  • Switch to CCD
  • Click record

• Drop screen to change modes
• Set ALLOWTOPICVIEW =

-- KdDerr - 27 Jul 2007