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| META TOPICPARENT | name="Jeol2100Instructions" |
Contents
MDS from Scratch
- Set up Search mode
- Start MDS, click Search
- Load "Basic" -- a good alignment of the microscope
- Mag mode 1 (NOT SADIFF)
- Set mag to 10K, and fully expand beam
- Go to SADIFF mode
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- Choose combination of camera length and Diff Focus to get correct mag of beam
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- Choose combination of camera length and Diff Focus to get correct mag and brightness (should just be short of covering CCD)
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- Move Free Lens Control slider all the way right to get dim beam (beam will move a little). Remember to hit "All Off" when going to Focus.
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- Recommended camera length 150-200 cm
- Adjust position of beam with PLA (NOT Image shift!!) to put beam over Fastscan camera
- If things are funny/messed up, go back to mag mode and fix
- Set up Focus mode
- Got to Focus in MDS
- Load "Basic" alignment
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- Set up mag/beam to desired level (200 K, spot 1, 10 pA/cm2 is usual)
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- Set up mag/beam to desired level (200 K, spot 1, 10 pA/cm2 is usual). Use Mag 1 or Mag 2, not SA Mag.
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- Adjust position of beam to be different from Photo-set position using image shift/beam shift as follows:
- Using Beam shift, move beam to very top of large screen
- Using Image shift, bring back down to center of screen
- Repeat. Repeat again if it seems too close for you.
- Beam is now centred over 2K camera. To set position of beam over Fastscan camera:
- a) approximate - use beam shift to move to correct position. Since position is not exactly centred, cannot be used reliably for stigmatism correction
- b) more exact: Use the "Off axis" macro in EMMenu3 to move beam using PLA. Remember to use "On axis" before taking picture, as PLA shift is applied to PhotoSet as well
- Set up PhotoSet mode:
- Go to PhotoSet in MDS
- Load "Basic" Alignment
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- Set up mag/beam as desired. Use 9-10 pA/cm2 for a 1-second exposure.
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- Set up mag/beam as desired. Use mag 1 or mag 2, not SA Mag. Use 9-10 pA/cm2 for a 1-second exposure.
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- Alignment of Search and PhotoSet
- Go to Search mode
- Find a clean area of ice to burn
- Go to Focus, then PhotoSet. Burn a nice hole using a long exposure with the screen down
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- Go back to Search. Centre hole using PLA shift (NOT image shift)
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- Go back to Search. Centre burn hole using PLA shift (NOT image shift)
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| | Deflectors used in MDS
- Mag Mode = Image Shift 1
- Diff Mode = PL Align
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> > | Other notes
- MDS remembers last mag set used, though this is not saved anywhere accessable
- If the last user messed things up by using non-standard settings or pushing the wrong button, MDS will have to be reprogrammed as described above.
- If you lose beam in Focus, remember to turn Free Lens Control to "All Off".
- Never use SA Mag, as the lens/delfector settings are different from Mag1/Mag2
- Monitor Lens/deflector levels. Remember that neutral is 8000 8000. Should be no image shift in PhotoSet mode, PLA should be neutral unless off-axis.
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< < | -- KdDerr - 08 Mar 2007 |
> > | -- BillRice - 12 Mar 2007 |