Maintenance Instructions for 14 day tasks

Week A

Vacuum F20 and 1230 rooms

Regrease O-rings and clean All Microscope Holders

• Only Use Fomblin Grease and Lint Free cloths
  • JEOL 1230 (1)
  • JEOL 2100 (3)
  • FEI F20 (5)

Fill U-traps on drains with water

• 2 locations
  • dark room
  • behind dishwasher

Check Vacuum Gauges on on Gatan Dry Pumping Station

• This is to ensure that the Cold Cathode Gauge is working
• Should read 10-4 range if pumped overnight
• If it does not clean the cold cathode gauge:
  • Turn off and unplug pumping station
  • Vent the system
  • Remove gauge by pulling (it is secured by vacuum and an o-ring)
  • Unscrew the black plastic nut on the bottom of the top gauge.
  • Open gauge.
  • Loosen and remove securing plate with two Hex screws
  • Remove pin
  • With the pin will come : 1 o-ring
  • Use a 3M pad to remove contamination from the pin
    • Use caution and prevent moving the small metal igniter its location is critical to the functioning of the gauge.
  • Dust with compressed air.
  • Reassemble the gauge.

Week B

Vacuum 2100 and 3200 rooms

Check Compressed gas tank regulators

• Open gas tank
• Observe pressure on right dial of regulator
• If at or below 500 psi check end of hallway for back up and list on dry erase board
• 9 compressed gas tanks
  • HPF
  • Ethane
  • Nitrogen for Plunge Freezer
  • CO2 for incubator
  • F20 Film exchange tank
  • 2100 ancillary hallway
  • 3200 ancillary hallway
  • Gatan Solarius gases (Ar, N, and O)

Check oil levels on all Mechanical pumps

• Observe level and color of oil in all mechanical pumps
• If oil shows yellow or darkening hue replace. Do not wait until it browns.
• 8 Locations - 10 pumps
  • BOC Edwards Vacuum Evaporator
  • JEOL Film pumping station - Microscope Suite Hallway
  • F20 Film pumping station - below phase II stairway
  • F20 Mechanical pump
  • 3200 Mechanical pump (2)
  • 2100 Mechanical pump (2)
  • 1230 Mechanical pump (1)
  • Jun-Aire Compressor -1230 Ancillary hallway

Check Chiller water levels

• Check 5 locations
  • 4 water chillers - one for each microscope
  • Incubator water level - Cell Culture Room

• Set ALLOWTOPICVIEW =

-- KdDerr - 04 Mar 2009