Difference: MicrotomeConversion (1 vs. 3)

Revision 321 Oct 2009 - Main.KdDerr

 
META TOPICPARENT name="CryoUltraMicrotomy"
Changed:
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<		Microtome Conversion
>
>		Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM
Added:
>
>

Principles & Protocols

Protocols - Microtome Conversion

 
Deleted:
<
<		Contents
 

Remove Cryosectioning unit

  • Bake for at least one hour before removing
  • Loosen thumb screw on specimen arm
  • Loosen both levers on right side
  • Unplug cable of left
  • Slide cryo unit towards yourself

Attaching room temperature sectioning unit

  • Slide plastic sectioning tray until plastic is flush with microtome frame

Removing room temperature unit

  • slide plastic tray towards yourself

Attaching cryosectioning unit

  • Press "FC Mount" on touch screen
  • Raise specimen arm to top position
  • Slide Cryo unit onto tracks
  • Connect sectioning arm with specimen holder
  • Slide cryo unit remainder of the way on
  • Leave a gap between back of chamber and heater about the thickness of a penny
  • Fasten specimen arm thumb screw
  • Make sure hand wheel can make a complete turn
  • Fasten two levers on right
  • Give a light tug to make sure they are secure
  • Fasten cable on left to back of machine
  • Connect LN2 hose
  • Insert specimen chuck
  • Insert knife holder
  • Turn on grey control unit
  • Cool down

Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Revision 209 Jun 2006 - Main.KdDerr

 
META TOPICPARENT name="CryoUltraMicrotomy"
Changed:
<
<		Microtome Conversion

Contents

Remove Cryosectioning unit

  • Bake for at least one hour before removing
  • Loosen thumb screw on specimen arm
  • Loosen both levers on right side
  • Unplug cable of left
  • Slide cryo unit towards yourself

Attaching room temperature sectioning unit

  • Slide plastic sectioning tray until plastic is flush with microtome frame

Removing room temperature unit

  • slide plastic tray towards yourself

Attaching cryosectioning unit

  • Raise specimen arm to top position
  • Slide Cryo unit onto tracks
  • Connect sectioning arm with specimen holder
  • Slide cryo unit remainder of the way on
  • Fasten specimen arm thumb screw
  • Make sure hand wheel can make a complete turn
  • Fasten two levers on right
  • Give a light tug to make sure they are secure
  • Fasten cable on left to back of machine
  • Connect LN2 hose
  • Insert specimen chuck
  • Insert knife holder
  • Turn on grey control unit
  • Cool down

Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

>
>		Microtome Conversion

Contents

Remove Cryosectioning unit

  • Bake for at least one hour before removing
  • Loosen thumb screw on specimen arm
  • Loosen both levers on right side
  • Unplug cable of left
  • Slide cryo unit towards yourself

Attaching room temperature sectioning unit

  • Slide plastic sectioning tray until plastic is flush with microtome frame

Removing room temperature unit

  • slide plastic tray towards yourself

Attaching cryosectioning unit

  • Press "FC Mount" on touch screen
  • Raise specimen arm to top position
  • Slide Cryo unit onto tracks
  • Connect sectioning arm with specimen holder
  • Slide cryo unit remainder of the way on
  • Leave a gap between back of chamber and heater about the thickness of a penny
  • Fasten specimen arm thumb screw
  • Make sure hand wheel can make a complete turn
  • Fasten two levers on right
  • Give a light tug to make sure they are secure
  • Fasten cable on left to back of machine
  • Connect LN2 hose
  • Insert specimen chuck
  • Insert knife holder
  • Turn on grey control unit
  • Cool down

Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

Added:
>
>

Revision 108 Jun 2005 - Main.KdDerr

 
META TOPICPARENT name="CryoUltraMicrotomy"
Microtome Conversion

Contents

Remove Cryosectioning unit

  • Bake for at least one hour before removing
  • Loosen thumb screw on specimen arm
  • Loosen both levers on right side
  • Unplug cable of left
  • Slide cryo unit towards yourself

Attaching room temperature sectioning unit

  • Slide plastic sectioning tray until plastic is flush with microtome frame

Removing room temperature unit

  • slide plastic tray towards yourself

Attaching cryosectioning unit

  • Raise specimen arm to top position
  • Slide Cryo unit onto tracks
  • Connect sectioning arm with specimen holder
  • Slide cryo unit remainder of the way on
  • Fasten specimen arm thumb screw
  • Make sure hand wheel can make a complete turn
  • Fasten two levers on right
  • Give a light tug to make sure they are secure
  • Fasten cable on left to back of machine
  • Connect LN2 hose
  • Insert specimen chuck
  • Insert knife holder
  • Turn on grey control unit
  • Cool down

Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

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