META FILEATTACHMENT	attachment="CoursePack.pdf" attr="" comment="All Course Materials: Single File" date="1184339350" name="CoursePack.pdf" path="CoursePack.pdf" size="66115219" stream="CoursePack.pdf" user="Main.JasperShahn" version="0"

Revision 513 Jul 2007 - Main.JasperShahn

META TOPICPARENT	name="MoD1"

Contents

See MOD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_1.pdf: Presented by Mike Goger, 9/8/2011

See MoD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

Lecture 2

* Module_2.pdf: Presented by Mike Goger, 9/8/2011 and 9/15/ 2011

Lecture 3

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_3.pdf: Module_3.pdf

Lecture 4

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_4.pdf: Module_4.pdf

Lecture 5

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_5.pdf: Module_5.pdf

Lecture 6

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_6_supp.pdf: Module_6_supp.pdf

* Module_6.pdf: Module_6.pdf

* Module_6_X.pdf: Module_6_X.pdf

References --

Lecture 7

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_7.pdf: Module_7.pdf

* Module_8.pdf: Module_8.pdf * Module_9.pdf: Module_9.pdf -- for lecture 9, Side-chain Assignments

Lecture 10 -- content see MoD9

* 3dmanual.pdf: 3dmanual.pdf -- for lecture 10 && 11 -- The through bond assignment strategy and triple res experiments how they work

2003 EMBO Course Notes
2005 EMBO Course Notes
Sattler Review Article Progress in Nuclear Magnetic Resonance Spectroscopy 34 (1999) 93–158

Module 12. Analyzing spectra for assignment etc. General review of approach. Use of NMRVIEW

Review and Midterm take home exam

Contents

Name of the topic

Sublevel topic

subsub level topic

Set ALLOWTOPICVIEW =

-- MichaelGoger - 20 Sep 2011

* classBBassign-II.ppt: classBBassign-II.ppt

Mid term test -- see midterm.pdf

Module 13. Advanced preparation issues -- Pichia, cell free, segmental labeling, strategy selection

proteinprep2.ppt: proteinprep2.ppt

Reference papers --

Protein expression systems for structural genomics and proteomics

Pichia

Production of ... proteins in ... Pichia pastoris

Process technology for ... proteins with Pichia pastoris

Cell Free

Comparison of Cell-Based and Cell-Free Protocols for Producing Target Proteins from the Arabidopsis thaliana Genome for Structural Studies

Segmental labeling

Segmental labeling using expressed protein ligation

Sigma segmental labeling

gyrA segmental labelling

Module 19 -- Illustrative example of calculation flow using Aria protocol

Added:

>
>

* CoursePack.pdf: All Course Materials: Single File

META FILEATTACHMENT	attachment="CoursePack.pdf" attr="" comment="All Course Materials: Single File" date="1184339350" name="CoursePack.pdf" path="CoursePack.pdf" size="66115219" stream="CoursePack.pdf" user="Main.JasperShahn" version="0"

Revision 406 Mar 2007 - Main.DavidCowburn

META TOPICPARENT	name="MoD1"

Contents

See MOD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_1.pdf: Presented by Mike Goger, 9/8/2011

See MoD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

Lecture 2

* Module_2.pdf: Presented by Mike Goger, 9/8/2011 and 9/15/ 2011

Lecture 3

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_3.pdf: Module_3.pdf

Lecture 4

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_4.pdf: Module_4.pdf

Lecture 5

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_5.pdf: Module_5.pdf

Lecture 6

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_6_supp.pdf: Module_6_supp.pdf

* Module_6.pdf: Module_6.pdf

Added:

>
>

* Module_6_X.pdf: Module_6_X.pdf

References --

Lecture 7

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_7.pdf: Module_7.pdf

* Module_8.pdf: Module_8.pdf * Module_9.pdf: Module_9.pdf -- for lecture 9, Side-chain Assignments

Lecture 10 -- content see MoD9

* 3dmanual.pdf: 3dmanual.pdf -- for lecture 10 && 11 -- The through bond assignment strategy and triple res experiments how they work

2003 EMBO Course Notes
2005 EMBO Course Notes
Sattler Review Article Progress in Nuclear Magnetic Resonance Spectroscopy 34 (1999) 93–158

Module 12. Analyzing spectra for assignment etc. General review of approach. Use of NMRVIEW

Added:

>
>

Review and Midterm take home exam

Contents

Name of the topic

Sublevel topic

subsub level topic

Set ALLOWTOPICVIEW =

-- MichaelGoger - 20 Sep 2011

* classBBassign-II.ppt: classBBassign-II.ppt

Mid term test -- see midterm.pdf

Module 13. Advanced preparation issues -- Pichia, cell free, segmental labeling, strategy selection

proteinprep2.ppt: proteinprep2.ppt

Reference papers --

Protein expression systems for structural genomics and proteomics

Pichia

Production of ... proteins in ... Pichia pastoris

Process technology for ... proteins with Pichia pastoris

Cell Free

Comparison of Cell-Based and Cell-Free Protocols for Producing Target Proteins from the Arabidopsis thaliana Genome for Structural Studies

Segmental labeling

Segmental labeling using expressed protein ligation

Sigma segmental labeling

gyrA segmental labelling

Module 19 -- Illustrative example of calculation flow using Aria protocol

Revision 306 Mar 2007 - Main.DavidCowburn

Added:

>
>

META TOPICPARENT	name="MoD1"

Contents

See MOD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_1.pdf: Presented by Mike Goger, 9/8/2011

See MoD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

Lecture 2

* Module_2.pdf: Presented by Mike Goger, 9/8/2011 and 9/15/ 2011

Lecture 3

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_3.pdf: Module_3.pdf

Lecture 4

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_4.pdf: Module_4.pdf

Lecture 5

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_5.pdf: Module_5.pdf

Lecture 6

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_6_supp.pdf: Module_6_supp.pdf

* Module_6.pdf: Module_6.pdf

Lecture 7

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_7.pdf: Module_7.pdf

* Module_8.pdf: Module_8.pdf * Module_9.pdf: Module_9.pdf -- for lecture 9, Side-chain Assignments

Lecture 10 -- content see MoD9

* 3dmanual.pdf: 3dmanual.pdf -- for lecture 10 && 11 -- The through bond assignment strategy and triple res experiments how they work

2003 EMBO Course Notes
2005 EMBO Course Notes
Sattler Review Article Progress in Nuclear Magnetic Resonance Spectroscopy 34 (1999) 93–158

Module 12. Analyzing spectra for assignment etc. General review of approach. Use of NMRVIEW

Contents

Name of the topic

Sublevel topic

subsub level topic

Set ALLOWTOPICVIEW =

-- MichaelGoger - 20 Sep 2011

* classBBassign-II.ppt: classBBassign-II.ppt

Mid term test -- see midterm.pdf

Module 13. Advanced preparation issues -- Pichia, cell free, segmental labeling, strategy selection

proteinprep2.ppt: proteinprep2.ppt

Reference papers --

Protein expression systems for structural genomics and proteomics

Pichia

Production of ... proteins in ... Pichia pastoris

Process technology for ... proteins with Pichia pastoris

Cell Free

Comparison of Cell-Based and Cell-Free Protocols for Producing Target Proteins from the Arabidopsis thaliana Genome for Structural Studies

Segmental labeling

Segmental labeling using expressed protein ligation

Sigma segmental labeling

gyrA segmental labelling

Module 19 -- Illustrative example of calculation flow using Aria protocol

Revision 206 Mar 2007 - Main.DavidCowburn

	Contents See MOD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_1.pdf: Presented by Mike Goger, 9/8/2011 See MoD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^? Lecture 2 * Module_2.pdf: Presented by Mike Goger, 9/8/2011 and 9/15/ 2011 Lecture 3 See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_3.pdf: Module_3.pdf Lecture 4 See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_4.pdf: Module_4.pdf Lecture 5 See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_5.pdf: Module_5.pdf Lecture 6 See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_6_supp.pdf: Module_6_supp.pdf * Module_6.pdf: Module_6.pdf Lecture 7 See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^? * Module_7.pdf: Module_7.pdf * Module_8.pdf: Module_8.pdf * Module_9.pdf: Module_9.pdf -- for lecture 9, Side-chain Assignments Lecture 10 -- content see MoD9 * 3dmanual.pdf: 3dmanual.pdf -- for lecture 10 && 11 -- The through bond assignment strategy and triple res experiments how they work 2003 EMBO Course Notes 2005 EMBO Course Notes Sattler Review Article Progress in Nuclear Magnetic Resonance Spectroscopy 34 (1999) 93–158
Added:
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	Module 12. Analyzing spectra for assignment etc. General review of approach. Use of NMRVIEW
Added:
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	Contents Name of the topic Sublevel topic subsub level topic Name of the topic Sublevel topic subsub level topic Set ALLOWTOPICVIEW = -- MichaelGoger - 20 Sep 2011
Added:
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	* classBBassign-II.ppt: classBBassign-II.ppt Mid term test -- see midterm.pdf Module 13. Advanced preparation issues -- Pichia, cell free, segmental labeling, strategy selection proteinprep2.ppt: proteinprep2.ppt Reference papers -- Protein expression systems for structural genomics and proteomics Pichia Production of ... proteins in ... Pichia pastoris Process technology for ... proteins with Pichia pastoris Cell Free Comparison of Cell-Based and Cell-Free Protocols for Producing Target Proteins from the Arabidopsis thaliana Genome for Structural Studies Segmental labeling Segmental labeling using expressed protein ligation Sigma segmental labeling gyrA segmental labelling Module 19 -- Illustrative example of calculation flow using Aria protocol Suggested Reading -- http://www.nysbc.net/library/Guntert03.pdf ; http://www.nysbc.net/library/RiepingHabeck07.pdf ; http://www.nysbc.net/library/LingeODonoghue01.pdf