See MOD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_1.pdf: Presented by Mike Goger, 9/8/2011

See MoD1 MoD2 MoD3 MoD4 MoD5 MoD6 MoD6X MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 MoD26 MoD27 MoD28 MoD29 MoD30 MoD31^?

Lecture 2

* Module_2.pdf: Presented by Mike Goger, 9/8/2011 and 9/15/ 2011

Lecture 3

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_3.pdf: Module_3.pdf

Lecture 4

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 MoD17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_4.pdf: Module_4.pdf

Lecture 5

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_5.pdf: Module_5.pdf

Lecture 6

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_6_supp.pdf: Module_6_supp.pdf

* Module_6.pdf: Module_6.pdf

* Module_6_X.pdf: Module_6_X.pdf

References --

1. http://www.nysbc.net/library/PackerWright83.pdf
2. http://www.nysbc.net/library/SorensenEich84.pdf

Lecture 7

See MoD2 MoD3 MoD4 MoD5 MoD6 MoD7 MoD8 MoD9 MoD10 MoD11 MoD12 MoD13 MoD12 MoD15 MoD16 Mod17 MoD18 MoD19 MoD20 MoD21 MoD22 MoD23 MoD24 MoD25 Mod26 MoD27 MoD28 MoD29 MoD30 MoD31^?

* Module_7.pdf: Module_7.pdf

* Module_8.pdf: Module_8.pdf * Module_9.pdf: Module_9.pdf -- for lecture 9, Side-chain Assignments

Lecture 10 -- content see MoD9

* 3dmanual.pdf: 3dmanual.pdf -- for lecture 10 && 11 -- The through bond assignment strategy and triple res experiments how they work

2003 EMBO Course Notes
2005 EMBO Course Notes
Sattler Review Article Progress in Nuclear Magnetic Resonance Spectroscopy 34 (1999) 93–158

Module 12. Analyzing spectra for assignment etc. General review of approach. Use of NMRVIEW

Review and Midterm take home exam

Contents

Name of the topic

Sublevel topic

subsub level topic

• Set ALLOWTOPICVIEW =

-- MichaelGoger - 20 Sep 2011

* classBBassign-II.ppt: classBBassign-II.ppt

Mid term test -- see midterm.pdf

Module 13. Advanced preparation issues -- Pichia, cell free, segmental labeling, strategy selection

• proteinprep2.ppt: proteinprep2.ppt

Reference papers --

Protein expression systems for structural genomics and proteomics

• Pichia

Process technology for ... proteins with Pichia pastoris

• Cell Free

Comparison of Cell-Based and Cell-Free Protocols for Producing Target Proteins from the Arabidopsis thaliana Genome for Structural Studies

• Segmental labeling

Segmental labeling using expressed protein ligation

Sigma segmental labeling

gyrA segmental labelling

Module 19 -- Illustrative example of calculation flow using Aria protocol

Suggested Reading -- http://www.nysbc.net/library/Guntert03.pdf ; http://www.nysbc.net/library/RiepingHabeck07.pdf ; http://www.nysbc.net/library/LingeODonoghue01.pdf