Difference: NaiderZerbe (4 vs. 5)

Revision 503 Apr 2008 - Main.DavidCowburn

 Set ALLOWTOPICVIEW = NmrfacGroup, NysbcadminGroup, FredNaider, OliverZerbe

META FORM name="ProposalForm"
FORM FIELD Project Title ProjectTitle GPCR peptide structures
FORM FIELD Principle Investigator PrincipleInvestigator Fred Naider
FORM FIELD Grant Source GrantSource
FORM FIELD Grant Number GrantNumber
FORM FIELD Grant Dates GrantDates
FORM FIELD Facilities Required FacilitiesRequired biochemical-facilities, solution-NMR
FORM FIELD Animal Origin AnimalOrigin neither
FORM FIELD Animal Species AnimalSpecies
FORM FIELD Animal Protocol Num AnimalProtocolNum
FORM FIELD Animal Protocol Date AnimalProtocolDate
FORM FIELD Human Protocol Num HumanProtocolNum
FORM FIELD Human Protocol Date HumanProtocolDate
Changed:
<
<		|*FORM FIELD Project Description*|ProjectDescription|I think we would need the 2D ct-13C,1H HSQC to check for slight shift changes, and to also be guided through the 3D in the end. I believe the 13C-NOESY is all we need at the moment. We will adjust our assignments on that spectrum. The sample is in D2O at the moment, so we will miss the NOEs to amides, but will have much better quality in the aliphatic range. We will run 13C-HSQC here and could send it to the NY structural biology center electronically, so that the HSQC could be directly overlayed. We could check sample integrity here any time before. |
>
>
FORM FIELD Project Description ProjectDescription

I would like to formally apply for measuring time on the 900 MHz instrument as discussed between Prof. Naider and Cowburn. The sample seems to be rather stable, and can certainly be stored in the fridge for periods of a couple of weeks. The 13C-NOESY needs to be measured at 320K, and for navigating through the 3D we would like to also have a constant-time 13C,1H HSQC. For the 2D 3-4 hours of measuring time are fine, for the 13C NOESY we expect to need approx. 3 days (16 scans) (at least). We would send the sample in the shigemi tube once the measuring slot has become clear. This will take approx. 3-4 days.

The sample is a doubly-labeled membrane protein (a GPCR fragment) in deuterated LPPG micelles. The protein concentration is approx. 400 uM, the detergent conc. is 200mM, pH about 6.

Pl. let me know if you need more information. I will provide more details on the sequences used here for the reference spectra, and the spectral widths, increments...etc

With kindest regards,

Oliver Zerbe

I think we would need the 2D ct-13C,1H HSQC to check for slight shift changes, and to also be guided through the 3D in the end. I believe the 13C-NOESY is all we need at the moment. We will adjust our assignments on that spectrum. The sample is in D2O at the moment, so we will miss the NOEs to amides, but will have much better quality in the aliphatic range. We will run 13C-HSQC here and could send it to the NY structural biology center electronically, so that the HSQC could be directly overlayed. We could check sample integrity here any time before. |
 
FORM FIELD Preliminary Results PreliminaryResults Lower field spectra
FORM FIELD Staff Notes StaffNotes Prelim to 900 use for Fred Naider
View topic  |  History: r7 < r6 < r5 < r4  |  More topic actions...
 
Copyright © by the contributing authors. All material on this collaboration platform is the property of the contributing authors.
Ideas, requests, problems regarding this intranet, Send feedback