Difference: PdsData (2 vs. 3)

Revision 330 Oct 2007 - Main.KdDerr

 
META TOPICPARENT name="FilmScreeningAndScanning"
Contents

Densitometer data

Startx -- -depth 8
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  Cd /home/nsfhome/kderr/pds-…

Change .HDR and .IMG files to lowercase

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  Mv HDR filename.hdr
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  Pds2mrc (for PDS)
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  New title-title-set #-film#-mag-machine-um size-date-speed-initials

Or (for Zeiss)

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  Open IMOD in another shell
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  Cd /cryoem/IMOD/IMOD-linux.csh
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  Tiff2mrc FILENAME.tif filename.mrc
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  New title-title-set #-film#-mag-machine-um size-date-speed-initials

Fftrans FILENAME.mrc filename.fft

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  Mrcdisp filename.fft
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  Sctravgft (scatter average ft)
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  ALL defaults OK
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  Enter file name [def.fft] filename
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  Sector [def 5]
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  Enter output name [def filename.ctf](ctf=contrast transfer function)
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  Table size [def 1024]
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  All sectors using defocus amount [def N]
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  Subctfbkg
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  Remove parenthesis with dialog on third line
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  Must rename file
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  Control C to exit
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  Use mv to move data back into original file
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  Gnuplot
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  Plot “filename.ctf”
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  Set xrange[0:512]
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  Set yrange[0:250]
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  Replot
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  Set title “collection parameters, date, initials”
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  Replot
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  Set term postscript color
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  Set output “filename.ctf.ps”
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  Replot
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  Set term x11
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  Quit
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  Lpr filename (to print)
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  Subctfbkg
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  Plot signal to noise ratio as .snr
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-- KdDerr - 30 Oct 2007

 
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