Difference: PdsDensitometer (1 vs. 2)

Revision 220 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="FilmScreeningAndScanning"

Added:

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Return to Cryo EM Web site at http://www.nysbc.org/facilities.CEM

Using the PDS 1010GM Microdensitometer

Download MSWord file with these instructions: PDS-1010GM-Usage.doc

Contents

To start

Put on gloves
Sweep glass with anti-static brush
- do not use canned air on glass
Clean film with quick burst of canned air
Place film on glass emulsion side up
Plug in vacuum pump for film holder
- pump located on other side of the wall but plug comes through the wall

PDS Alignment

Select "7" on inner filter wheel (protects PMT)
Turn on Power, Lamp, PMT and allow to warm up
Select upper and lower apertures and lenses depending on required pixel size
- i.e. For 13 um scan:
  - Use "B" aperture" and "2" lens both above and below the film
- For 6.5 um scan
  - Use "B" aperture and "4" lens above and below the film
Move to clear area on film
Focus lower lens until see sharp edges of lower aperture on screen
Remove aperture with lower left dial
Focus upper lens until film grain is sharp (not lamp fiber optics!)
Replace aperture
Center upper lens roughly on main screen- with 4 set screws
Check center with monocular (upper aperture should be inside lower aperture)
Set gain:
- Select "C" on filter wheels (both)
- Toggle to TRANS, CALIB to 1
- adjust PMT voltage -> 1.00
Toggle to DENS, CALIB to N
- adjust LOG -> 0.06
Leave in DENS - N mode
Select "7" on inner filter wheel for safety - especially with small apertures
Move to center of desired scan
Reset X-Y positions with red push buttons
Remove aperture with lower dial
Check lens focus on film grain using monocular eyepiece
Replace aperture
Select "C" on filter wheel
Push SELECT -> AUTO

Computer Controls

Put computer in MSDOS mode
- Either choose MSDOS mode upon startup
- Or choose "Shutdown" and specify "Start in MSDOS mode"
Start SCANS-A-LOT control program
- cd c:\pds
- scans
look over configuration, press ENTER
Enter "ED" to edit scan
"2" to change name of file
"3" to change scan parameters and speed
"ctrl + Z" to go back to edit menu
"0" to exit out of edit menu
"h" to display Scans-A-Lot prompts
"CS" to begin scan
"Q" to exit to windows

Finish scan

Turn off PMT, Lamp, Power and recover PDS when finished

Transfer files to dl380

start Windows
- type "win" at MSDOS command prompt
click winscp361.exe
Copy files from c:\pds on PDS computer to dl380 /home/your file folder
- Via drag and drop
Delete files from PDS computer after confirmed transfer

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

META FILEATTACHMENT	attr="" comment="" date="1111185534" name="PDS-1010GM-Usage.doc" path="PDS-1010GM-Usage.doc" size="33280" user="DavidStokes" version="1.1"

Revision 118 Mar 2005 - Main.DavidStokes

META TOPICPARENT	name="FilmScreeningAndScanning"

Using the PDS 1010GM Microdensitometer

Download MSWord file with these instructions: PDS-1010GM-Usage.doc

Contents

To start

Put on gloves
Sweep glass with anti-static brush
- do not use canned air on glass
Clean film with quick burst of canned air
Place film on glass emulsion side up
Plug in vacuum pump for film holder
- pump located on other side of the wall but plug comes through the wall

PDS Alignment

Select "7" on inner filter wheel (protects PMT)
Turn on Power, Lamp, PMT and allow to warm up
Select upper and lower apertures and lenses depending on required pixel size
- i.e. For 13 um scan:
  - Use "B" aperture" and "2" lens both above and below the film
- For 6.5 um scan
  - Use "B" aperture and "4" lens above and below the film
Move to clear area on film
Focus lower lens until see sharp edges of lower aperture on screen
Remove aperture with lower left dial
Focus upper lens until film grain is sharp (not lamp fiber optics!)
Replace aperture
Center upper lens roughly on main screen- with 4 set screws
Check center with monocular (upper aperture should be inside lower aperture)
Set gain:
- Select "C" on filter wheels (both)
- Toggle to TRANS, CALIB to 1
- adjust PMT voltage -> 1.00
Toggle to DENS, CALIB to N
- adjust LOG -> 0.06
Leave in DENS - N mode
Select "7" on inner filter wheel for safety - especially with small apertures
Move to center of desired scan
Reset X-Y positions with red push buttons
Remove aperture with lower dial
Check lens focus on film grain using monocular eyepiece
Replace aperture
Select "C" on filter wheel
Push SELECT -> AUTO

Computer Controls

Put computer in MSDOS mode
- Either choose MSDOS mode upon startup
- Or choose "Shutdown" and specify "Start in MSDOS mode"
Start SCANS-A-LOT control program
- cd c:\pds
- scans
look over configuration, press ENTER
Enter "ED" to edit scan
"2" to change name of file
"3" to change scan parameters and speed
"ctrl + Z" to go back to edit menu
"0" to exit out of edit menu
"h" to display Scans-A-Lot prompts
"CS" to begin scan
"Q" to exit to windows

Finish scan

Turn off PMT, Lamp, Power and recover PDS when finished

Transfer files to dl380

start Windows
- type "win" at MSDOS command prompt
click winscp361.exe
Copy files from c:\pds on PDS computer to dl380 /home/your file folder
- Via drag and drop
Delete files from PDS computer after confirmed transfer

Visit Cryo EM Web site at http://www.nysbc.org/facilities/CEM

-- DavidStokes - 18 Mar 2005

META FILEATTACHMENT	attr="" comment="" date="1111185534" name="PDS-1010GM-Usage.doc" path="PDS-1010GM-Usage.doc" size="33280" user="DavidStokes" version="1.1"

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