Difference: PreliminaryResults (1 vs. 2)
Revision 203 Apr 2008 - Main.DanaMoses
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| < < | Preliminary results (eg low field NMR) related to NYSBC involvement | |||||||
| > > | Rad51 filaments on ssDNA have been formed, both with yeast and human proteins, as well as with wildtype and recombination-mutant forms of the proteins. These filaments have been shown to be stable for up to several hours. These filaments have also been shown to be active in recombination by several measures. DNA molecules labeled with quantum dots can be separated from unlabeled DNA using ion exchange chromatography, and this method is currently being refined in our lab. Once this purification is in place, we will be able to isolate labeled DNA molecules or nucleoprotein filaments. | |||||||
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| > > | We will soon be able to produce stable, active, fluorescently labeled filaments. At that time, we will be able to inject these into our flow cells containing dsDNA and observe the homology search in real time. We would like to visualize DNA molecules bound to individual quantum dots to demonstrate that this is indeed what we have formed, and what we are injecting into our flow cells. We have so far used AFM to inspect our samples, and have had some success but believe that EM might be a better technique for imaging these samples. | |||||||
Revision 120 Feb 2008 - Main.DavidCowburn
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