Small Angle Scattering Primer

SAXS BNL Presentation

• NYSBC_presentation-s.pdf: NYSBC_presentation-s.pdf

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Small Angle Scattering Primer

SAXS BNL Presentation

• NYSBC_presentation-s.pdf: NYSBC_presentation-s.pdf

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Small Angle Scattering Primer

SAXS BNL Presentation

• NYSBC_presentation-s.pdf: NYSBC_presentation-s.pdf

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

* PartOne_final.pdf: NIH 2009 part 1

Small Angle Scattering Primer

SAXS BNL Presentation

• NYSBC_presentation-s.pdf: NYSBC_presentation-s.pdf

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Small Angle Scattering Primer

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants:
  Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source
   2. Simultaneous SAXS/WAXS
   3. Time-resolved experiments
   4. High-Throughput sample handling
3. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data
   2. Structural dynamics
4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?
   1. Basic theory of SAXS/WAXS
   2. Size and shape of macromolecules, e.g. Monomer-Dimer of SARS protease
   3. Molecular envelope and modeling, e.g. Gelsolin structure as a function of [Ca2+]
   4. Secondary and tertiary structure by WAXS, e.g. Structural changes due to ligand binding
2. How to measure SAXS/WAXS

1. Collaborative opportunities

1. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?

1. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling
2. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data b. Structural dynamics
3. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Special meeting, Monday Jun 28, 200P, A-11 NYSBC

1. What SAXS/WAXS can tell you?

1. How to measure SAXS/WAXS
   1. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling
2. Collaborative opportunities
   1. Molecular modeling based on complementary structural biology data b. Structural dynamics
3. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

• BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division
  Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9
  Dr. Xinguo Hong, Scientific Associate
  Dr. Marc Allaire, Scientist, Structural Biology
  Dr. Chi-Chang Kao (tentative), NSLS Chairman
  

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

NMR specific applications

New SANS resources

Outline for NYSBC SAXS presentation June 29, 2009

Outline

1. What SAXS/WAXS can tell you?

a. Basic theory of SAXS/WAXS b. Size and shape of macromolecules e.g. Monomer-Dimer of SARS protease c. Molecular envelope and modeling e.g. Gelsolin structure as a function of [Ca2+] d. Secondary and tertiary structure by WAXS e.g. Structural changes due to ligand binding

2. How to measure SAXS/WAXS a. Synchrotron beamline vs Home source b. Simultaneous SAXS/WAXS c. Time-resolved experiments d. High-Throughput sample handling

3. Collaborative opportunities a. Molecular modeling based on complementary structural biology data b. Structural dynamics

4. Discussion on beamtime access General proposal, Rapid Access, Mail-In, Contributing User, Proprietary User

BNL participants: Dr. Lisa Miller, NSLS Head of the Life and Environmental Sciences Division Dr. Lin Yang, Beamline Scientist, Spokeperson for beamline X9 Dr. Xinguo Hong, Scientific Associate Dr. Marc Allaire, Scientist, Structural Biology Dr. Chi-Chang Kao (tentative), NSLS Chairman

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

New SANS resources

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541
• Q Rev Biophys. 2007 Aug;40(3):191-285. X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Putnam CD, Hammel M, Hura GL, Tainer JA.

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571
• Small angle neutron and X-ray scattering in structural biology: recent examples from the literature Cameron NeylonEur Biophys J (2008) 37:531–541

Small Angle Scattering Primer

Broad reviews

• Analysis of X-ray and neutron scattering from biomacromolecular solutions ; Maxim V Petoukhov and Dmitri I Svergun, Curr Op Str Bio 2007, 17:562–571