TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

All groups who are interested in enabling biomedical research are encouraged to deposit their data to TargetTrack. Note that once a target is deposited to TargetTrack, it is considered public domain and cannot be removed from the database.

http://www.sbkb.org/tt/index.html

* TargetTrack is updated every Friday

Targets

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

* ralph_oct18.ppt: ralph_oct18.ppt

* temimps_target_track_oct2011: 2nd submission to TargetTrack (October 2011)

* temimps_pepcdb_oct2011: PepcDB^? submission (October 2011)

* temimps_targetdb_oct2011: TargetDB^? submission (October 2011)

* TEMIMPS_pepcdb_Nov2011: PepcDB^? submission (November 2011)

* TEMIMPS_targettrack_Nov2011: TargetDB^? submission (November 2011)

* TEMIMPS_TargetTrack_04-20-12: TargetTrack deposition (April 20, 2012)

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

Targets

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011 * temimps_targettrack_july2011: 1st submission to TargetTrack (July 2011)

* ralph_oct18.ppt: ralph_oct18.ppt

* temimps_target_track_oct2011: 2nd submission to TargetTrack (October 2011)

* temimps_pepcdb_oct2011: PepcDB^? submission (October 2011)

* temimps_targetdb_oct2011: TargetDB^? submission (October 2011)

* TEMIMPS_pepcdb_Nov2011: PepcDB^? submission (November 2011)

* TEMIMPS_targettrack_Nov2011: TargetDB^? submission (November 2011)

* TEMIMPS_TargetTrack_04-20-12: TargetTrack deposition (April 20, 2012)

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

Targets

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011 * temimps_targettrack_july2011: 1st submission to TargetTrack (July 2011)

* ralph_oct18.ppt: ralph_oct18.ppt

* temimps_target_track_oct2011: 2nd submission to TargetTrack (October 2011)

* temimps_pepcdb_oct2011: PepcDB^? submission (October 2011)

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011 * temimps_targettrack_july2011: 1st submission to TargetTrack (July 2011)

* ralph_oct18.ppt: ralph_oct18.ppt

* temimps_target_track_oct2011: 2nd submission to TargetTrack (October 2011)

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

Our Targets

Protein ID	Protein name	Status
APB	presenilin homolog	cloned, expressed, purified

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011 * temimps_targettrack_july2011: 1st submission to TargetTrack (July 2011)

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

Our Targets

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule:

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

Our Targets

d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

TargetTrack is designed to improve readibility of the experimental data provided, and to better support PSI:Biology and other new structural biology research activities. d23 1 New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule: The current database update schedule is as follows:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

References...

• xxx:
  • doc: Kuehlbrandt_table_partial.doc
  • excel: Kuhlbrandt_Lasala_Table.xlsx

• original Kuehlbrandt-table_list.xls: xxx.xls - xxx (xxx)

Our Targets

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011

TargetTrack

TargetTrack, a target registration database, provides information on the experimental progress and status of targets selected for structural determination by the Protein Structure Initiative and other worldwide high-throughput structural biology projects.

http://beta.sbkb.org/tt/

It is a merger of TargetDB^? and PepcDB^?.

TargetTrack is designed to improve readibility of the experimental data provided, and to better support PSI:Biology and other new structural biology research activities. d23 1 New features in TargetTrack include:

* Ability to define complex targets, including targets made up of multiple protein and/or nucleic acid sequences, or targets comprised of references to existing single-sequence targets * Ability to define one or more ligands bound to the target, either at time of selection or during structural studies * New data items to capture biological information about the target, such as "target rationale", "target annotation", and "target protein type" * New data item "target partnership" to capture PSI:Biology collaboration information * Expanded controlled vocabulary for "status", "target category", "protocol type", "sequence type", and other enumeration data items

The current schemas for TargetDB^? and PepcDB^? will continue to be supported for select activities (such as PSI-2 and non-PSI projects); and TargetTrack will be backwards compatible for these files.

TargetDB^?/PepcDB and TargetTrack will operate in parallel until the end of 2011. TargetDB^?/PepcDB updates are still being accepted for the duration of this transition period.

Current database update schedule: The current database update schedule is as follows:

* PepcDB^? is updated every Wednesday

* TargetDB^? is updated every Thursday d82 2 * TargetTrack is updated every Friday

References...

• xxx:
  • doc: Kuehlbrandt_table_partial.doc
  • excel: Kuhlbrandt_Lasala_Table.xlsx

• original Kuehlbrandt-table_list.xls: xxx.xls - xxx (xxx)

Our Targets

Crystallization information

• dope (mix with a bit of another lipids, about 10 %)
• begin with a mixture of detergents

Protein acquisition form: Protein_acquisition_form-Mar11.doc.

TEMIMPS targets timeline

• MHJF1 (X77-79) - detergent screening at 4, 25 degrees and with CD
• MCMJR1 (X??) - screening with protein in OG

August 2011

• MCMJR1 (X75)

July 2011

• EmrE Lactobacillus plantarum (X73)
• Yiip tests (having a reliable control)

June 2011

• EmrE shewanella oneidensis (X72)
• EmrE Lactobacillus plantarum (X71)
• MHJF1 experiments (X70) in FC12, and C12E8 tests (+BB).
• MCMJR1 (X69) back in FC-12 at room temp, and C12E8 tests (+BB).

May 2011

• MCMJR1 (X68; EM178-179-180) back in FC-12 at 4 degrees, and C12E8 tests (+BB).

April 2011

• TRP (X67, EM.174)

Mar 2011

• TRP channel (EM.167 and 169, X64)
• MCMJR1 (X66, EM.166)

• MCMJR1 (EM.161, X64)

January 2011

• MCMJR1 (EM.143, X63)

December 2010

• Transporter^?, CysZ (EM.126, X62)

2007-2009 - Summary of previous crystallization trials

• MCV crystallization Trials 2007-2009
• MMS2 crystallization Trials 2007-2009
• MHJF crystallization Trials 2007-2009
• MCMJR1 crystallization Trials 2007-2009

a96 1 d107 2

• Set ALLOWTOPICVIEW =

-- RalphLasala - 01 May 2012

-- RalphLasala - 01 Jul 2011