Difference: TemimpsJobs (1 vs. 15)

Current Job Openings at NYSBC

Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biology Center

Postdoctoral Position for a Membrane Protein Electron Microscopist

A postdoctoral position for a highly motivated electron microscopist is available immediately in the laboratory of David Stokes at the New York Structural Biology Center (http://cryoem.nysbc.org) in New York City. The successful applicant will work for the Transcontinental Electron Microscopy Initiative for Membrane Protein Structure (TEMIMPS; http://temimps.nysbc.org). TEMIMPS is funded by the NIH Protein Structure Initiative for development and implementation of high-throughput and high-resolution approaches to structure determination of membrane proteins using electron cryo-microscopy and 2D membrane crystals. TEMIMPS is composed of six research groups with combined expertise in membrane protein production, membrane crystallization, electron microscopy, image processing, and structure determination. Groups at Mt. Sinai School of Medicine, New York University and NYSBC work closely together. The group at Mt. Sinai focuses on production of purified membrane protein targets, whereas the group at NYSBC uses a high-throughput pipeline to screen crystallization conditions. The open position at NYSBC will involve crystallization of membrane proteins 3D reconstruction using methods of electron crystallography. The NYSBC is well equipped for all aspects of this project and hosts superlative facilities for electron cryo-microscopy. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Strong research experience in membrane protein biochemistry is highly desirable. In addition, the candidate must have strong interpersonal, organizational and communication skills in order to facilitate intimate collaboration with other research groups. Salary will be commensurate with experience. Interested candidates should send a cover letter, curriculum vitae and contact information for three professional referees to Dr. David Stokes at stokes@nysbc.org.

Jobs Available at NYSBC

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

A number of jobs are available in our new NIH-funded PSI center for Membrane Protein Structure Determination (http://temimps.nysbc.org). Our's is the only PSI center exclusively committed to determination of membrane protein structures using electron microscopy. This will involve development of a number of associated technologies, from high-throughput crystallization screening, automated imaging in the electron microscope, novel approaches for image analysis. Our overall goal is to solve structures over the 5-year period of the grant. The target list includes a wide variety of biologically important proteins, including transporters, pumps, signaling molecules, proteases and adhesion proteins.

Electron microscopist at New York University, New York, NY

Postdoctoral position is available immediately for an an electron crystallographer in the laboratory of David Stokes at the Skirball Institute of New York University School of Medicine (http://skirball.med.nyu.edu/~stokes). This position will focus on structure determination of tubular crystals formed by membrane proteins. This work will involve optimization of crystals discovered in the TEMIMPS high-throughput crystallization pipeline, which includes a broad array of novel membrane protein targets (http://temimps.nysbc.org). Structure determination will then involve imaging of frozen-hydrated samples and helical reconstruction using either Fourier Bessel methods or Interative Real Space methods. State-of-the-art cryo-electron microscopes are available both at NYU and at the New York Structural Biology Center for this work. Software for both approaches to helical reconstruction are a development focus for members of the TEMIMPS consortium. Candidate should have a Ph.D. with experience either in electron microscopy or image analysis and an ability to work effectively within the highly collaborative environment of our multi-institutional consortium. To apply, email curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nyu.edu).

d22 1

Jobs Available at NYSBC

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

A number of jobs are available in our new NIH-funded PSI center for Membrane Protein Structure Determination (http://temimps.nysbc.org). Our's is the only PSI center exclusively committed to determination of membrane protein structures using electron microscopy. This will involve development of a number of associated technologies, from high-throughput crystallization screening, automated imaging in the electron microscope, novel approaches for image analysis. Our overall goal is to solve structures over the 5-year period of the grant. The target list includes a wide variety of biologically important proteins, including transporters, pumps, signaling molecules, proteases and adhesion proteins.

Electron crystallographer at Mount Sinai School of Medicine - New York, NY

This postdoctoral position will focus on membrane protein structure determination using electron crystallography. This work will involve de novo crystallization and optimization of 2D crystals, followed by cryo-electron microscopy and 3D reconstruction using crystallogrpahic software tools. The department of Structural and Chemical Biology at Mount Sinai School of Medicine (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/) is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. In addition, we have access to superlative electron cryo-microscopy facilities at of the New York Structural Biology Center (http://cryoem.nysbc.org). Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in membrane protein structure determination by electron cryo-microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

Electron microscopist at New York University, New York, NY

Postdoctoral position at University of Washington School of Medicine, Seattle, WA

Postdoctoral fellow position is available in the laboratory of Tamir Gonen to study the structure and function of membrane transporters. This position is a mix of membrane biochemistry and electron crystallography/structure determination as described above. More information can be found at http://cryoem.washington.edu. Candidates should email curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Tamir Gonen (tgonen@u.washington.edu).

Postdoctoral position at University of Washington School of Medicine, Seattle, WA

Postdoctoral fellow position is available in the laboratory of Tamir Gonen to establish new protocols for structure determination in electron crystallography. This is a collaborative effort with Professor David Baker and is computationally intensive. More information can be found at http://cryoem.washington.edu. Candidates should hold a Ph.D. degree and have relevant experience. To apply, email curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Tamir Gonen (tgonen@u.washington.edu).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

The EM Facility at the Cleveland Center for Membrane and Structural Biology (CCMSB) and the Department of Pharmacology at Case Western Reserve University (CWRU) is seeking a Software Engineer. This individual will be the key researcher for building up the computing infrastructure for the EM facility of the Cleveland Center for Membrane and Structural Biology, CCMSB. The task includes building up and maintaining the computational environment for high-speed digital image processing of electron micrographs using software packages developed by the EM community such as 2DX, EMAN, IPLT, SPIDER. Further, new software will be developed within the TEMIMPS consortium, tested, and introduced. Projects are focused on the elucidation of the structure of membrane proteins. Expertise in Unix, C++ and background in math are required. We provide a great scientific environment within which the engineer can pick collaborative projects of interest. If you are interested, or for further information, please contact: Andreas Engel <ahe13@case.edu> or Vera Moiseenkova-Bell <vxm102@case.edu>

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

d22 1

Jobs Available at NYSBC

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Electron crystallographer at Mount Sinai School of Medicine - New York, NY

Electron microscopist at New York University, New York, NY

Software Engineer at Case Western Reserve University - Cleveland, Ohio

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

d22 1

Jobs Available at NYSBC

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

Project Manager at the New York Structural Biology Center, New York, NY

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience and, although the initial appointment is for one year, it will be renewable upon mutual consent. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org).

Software Engineer at New York University, New York, NY

Position for a software engineer is available immediately at the New York Structural Biology Center in New York. This position will contribute to our development of 2D crystallization of membrane proteins. The TEMIMPS consortium is developing automated, high-throughput methods to screen a large number of targets, thus making this process more systematic and to understand the parameters that have the greatest influence. We seek an individual to develop software to interface robotic hardware with web-based databases and electron microscopes. This work will involve development of computer vision and image processing algorithms and integrating these algorithms into existing software programs. This work will also involve structure determination of membrane proteins using methods of cryo-electron microscopy and electron crystallography. Candidates must hold a degree in Computer Science, Biophysics, Structural Biology or a related field and must feel comfortable working within a tight knit group at NYU, NYSBC and Mt. Sinai School of Medicine that handles the various aspects of our crystallization pipeline. This staff member will report to David Stokes at NYU School of Medicine. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nyu.org).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

Electron crystallographer at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated electron crystallographer is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on membrane protein structure determination using electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve de novo crystallization and optimization of 2D crystals. This work will also involve structure determination of membrane proteins using methods of cryo-electron microscopy and electron crystallography. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. In addition, we have fluid access to superlative electron cryo-microscopy facilities at of the New York Structural Biology Center (http://cryoem.nysbc.org). Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in membrane protein structure determination by electron cryo-microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

d22 1

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Project Manager at the New York Structural Biology Center, New York, NY

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience and, although the initial appointment is for one year, it will be renewable upon mutual consent. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org).

Software Engineer at New York University, New York, NY

Position for a software engineer is available immediately at the New York Structural Biology Center in New York. This position will contribute to our development of 2D crystallization of membrane proteins. The TEMIMPS consortium is developing automated, high-throughput methods to screen a large number of targets, thus making this process more systematic and to understand the parameters that have the greatest influence. We seek an individual to develop software to interface robotic hardware with web-based databases and electron microscopes. This work will involve development of computer vision and image processing algorithms and integrating these algorithms into existing software programs. This work will also involve structure determination of membrane proteins using methods of cryo-electron microscopy and electron crystallography. Candidates must hold a degree in Computer Science, Biophysics, Structural Biology or a related field and must feel comfortable working within a tight knit group at NYU, NYSBC and Mt. Sinai School of Medicine that handles the various aspects of our crystallization pipeline. This staff member will report to David Stokes at NYU School of Medicine. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nyu.org).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).

Electron crystallographer at Mount Sinai School of Medicine - New York, NY

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Project Manager at the New York Structural Biology Center, New York, NY

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience and, although the initial appointment is for one year, it will be renewable upon mutual consent. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org).

Software Engineer at New York University, New York, NY

Position for a software engineer is available immediately at the New York Structural Biology Center in New York. This position will contribute to our development of 2D crystallization of membrane proteins. The TEMIMPS consortium is developing automated, high-throughput methods to screen a large number of targets, thus making this process more systematic and to understand the parameters that have the greatest influence. We seek an individual to develop software to interface robotic hardware with web-based databases and electron microscopes. This work will involve development of computer vision and image processing algorithms and integrating these algorithms into existing software programs. This work will also involve structure determination of membrane proteins using methods of cryo-electron microscopy and electron crystallography. Candidates must hold a degree in Computer Science, Biophysics, Structural Biology or a related field and must feel comfortable working within a tight knit group at NYU, NYSBC and Mt. Sinai School of Medicine that handles the various aspects of our crystallization pipeline. This staff member will report to David Stokes at NYU School of Medicine. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nyu.org).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Project Manager at the New York Structural Biology Center, New York, NY

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience and, although the initial appointment is for one year, it will be renewable upon mutual consent. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org).

Software Engineer at New York University, New York, NY

Position for a software engineer is available immediately at the New York Structural Biology Center in New York. This position will contribute to our development of 2D crystallization of membrane proteins. The TEMIMPS consortium is developing automated, high-throughput methods to screen a large number of targets, thus making this process more systematic and to understand the parameters that have the greatest influence. We seek an individual to develop software to interface robotic hardware with web-based databases and electron microscopes. This work will involve development of computer vision and image processing algorithms and integrating these algorithms into existing software programs. This work will also involve structure determination of membrane proteins using methods of cryo-electron microscopy and electron crystallography. Candidates must hold a degree in Computer Science, Biophysics, Structural Biology or a related field and must feel comfortable working within a tight knit group at NYU, NYSBC and Mt. Sinai School of Medicine that handles the various aspects of our crystallization pipeline. This staff member will report to David Stokes at NYU School of Medicine. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nyu.org).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Jobs Available in the Transcontinental EM Initiative for Membrane Protein Structure

Project Manager at the New York Structural Biology Center, New York, NY

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Project Manager at the New York Structural Biology Center, New York, NY

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience and, although the initial appointment is for one year, it will be renewable upon mutual consent. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org). The New York Structural Biology Center is an equal opportunity employer committed to diversity.

Postdoctoral Associate at the New York Structural Biology Center, New York, NY

A postdoctoral position is available immediately at the New York Structural Biology Center in New York. This position will focus primarily on mechanisms of 2D crystallization of membrane proteins. The TEMIMPS consortium will be using automated, high-throughput methods to screen a large number of targets, thus providing an opportunity to make this process more systematic and to understand the parameters that have the greatest influence. In addition to establishing conditions for forming 2D crystals, there will also be opportunities for structure determination using methods of cryo-electron microscopy and electron crystallography. Candidates must hold a Ph.D. in Biochemistry, Biophysics, Structural Biology or a related field and must feel comfortable working within a tight knit group that handles the various aspects of our crystallization pipeline. This staff member will report to David Stokes, Director of Cryo-Electron Microscopy at NYSBC. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. David Stokes (stokes@nysbc.org). The New York Structural Biology Center is an equal opportunity employer committed to diversity.

Software Engineer at Case Western Reserve University - Cleveland, Ohio

We seek a software engineer who is a Ph.D. level researcher, has broad computing skills and an understanding of structural biology. This position will focus on implementation and/or refinement of tools for electron crystallography in the newly developed software package IPLT, adaptation and refinement of software for automated sample screening, and implementation/adaptation of software that controls a 2D crystallization-robot. Moreover, this specialist will be responsible for working with other members of the TEMIMPS consortium (see http://temimps.nysbc.org) to implement and maintain software packages developed at the other affiliated laboratories. This staff member will report to Dr. Andreas Engel, Director of the Cleveland Center for Membrane and Structural Biology. This position is available immediately and salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Andreas Engel (andreas.engel@case.edu).

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Jobs Available in the

Project Manager at the New York Structural Biology Center, New York, NY

Postdoctoral Associate at the New York Structural Biology Center, New York, NY

Software Engineer at Case Western Reserve University - Cleveland, Ohio

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Jobs Available in the

• Set ALLOWTOPICVIEW =

Jobs Available in the

Project Manager at the New York Structural Biology Center

Position for a Project Manager is available immediately at the New York Structural Biology Center in New York. This position will focus on the development of high-throughput approaches to 2D crystallization of membrane proteins by cryo-electron microscopy. The New York Structural Biology Center offers a highly collaborative environment with a wide range of researchers from around New York City. in addition to TEMIMPS, NYSBC houses the NYCOMPS PSI center, which focuses on X-ray crystallographic approaches to membrane protein structure and which is also funded by the NIH Protein Structure Initiative. Thus, NYSBC provides excellent facilities for membrane protein biochemistry, cryo-electron microscopy and image analysis. For our project on 2D crystallography, we have developed a parallelized, robotic approach to crystallizing and screening 2D crystallization, and this approach has potential to increased our throughput to an unprecedented level. The successful candidate will continue to develop our pipeline for expression, purification, and crystallization of a variety of membrane proteins and their complexes. Ultimately, we aim to use this technology to solve novel membrane protein structures. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or a related field. Strong research experience in structure determination of membrane proteins or in cryo-electron microscopy is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as this position will involve supervision of other laboratory staff. We offer a salary commensurate with experience and a competitive benefits package. The initial appointment is for one year and renewable upon mutual consent. Interested candidates should e-mail a PDF including a cover letter, full CV and contact information of three professional referees to temimps@nysbc.org. Applications will be reviewed as they are received until the position is filled. The New York Structural Biology Center is an equal opportunity employer committed to diversity.

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• Set ALLOWTOPICVIEW =