Difference: TemimpsJobs (12 vs. 13)

Electron crystallographer at Mount Sinai School of Medicine - New York, NY

Postdoctoral position at University of Washington School of Medicine, Seattle, WA

Postdoctoral position at University of Washington School of Medicine, Seattle, WA

Postdoctoral fellow position is available in the laboratory of Tamir Gonen to establish new protocols for structure determination in electron crystallography. This is a collaborative effort with Professor David Baker and is computationally intensive. More information can be found at http://cryoem.washington.edu. Candidates should hold a Ph.D. degree and have relevant experience. To apply, email curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Tamir Gonen (tgonen@u.washington.edu).

Software Engineer at Case Western Reserve University - Cleveland, Ohio

The EM Facility at the Cleveland Center for Membrane and Structural Biology (CCMSB) and the Department of Pharmacology at Case Western Reserve University (CWRU) is seeking a Software Engineer. This individual will be the key researcher for building up the computing infrastructure for the EM facility of the Cleveland Center for Membrane and Structural Biology, CCMSB. The task includes building up and maintaining the computational environment for high-speed digital image processing of electron micrographs using software packages developed by the EM community such as 2DX, EMAN, IPLT, SPIDER. Further, new software will be developed within the TEMIMPS consortium, tested, and introduced. Projects are focused on the elucidation of the structure of membrane proteins. Expertise in Unix, C++ and background in math are required. We provide a great scientific environment within which the engineer can pick collaborative projects of interest. If you are interested, or for further information, please contact: Andreas Engel <ahe13@case.edu> or Vera Moiseenkova-Bell <vxm102@case.edu>

Cryo-EM Lab Manager at University of Washington School of Medicine - Seattle, Washington

A position is available immediately for a Manager for the Cryo-EM lab at the University of Washington School of Medicine. This staff member will report to Dr. Tamir Gonen, Assistant Professor of Biochemistry and Howard Hughes Early Career Scientist. Salary will be commensurate with experience. Candidates should send their curriculum vitae, an application letter and names of three professional referees to temimps@nysbc.org or to Tamir Gonen (tgonen@u.washington.edu).

Duties and responsibilities:

• Design, implement, and maintain the CryoEM laboratory, including all ancillary equipment
• Oversee repair work and manage major service contracts for all electron microscopy equipment
• Train new users in the operation of the microscopes and related equipment. This will require the manager to be an expert in the equipment and its use.
• Work independently and/or in support of various users as required
• Perform data collection (image and diffraction), processing, and analysis tasks as needed
• Implement, utilize, and maintain user policies, scheduling system and microscopes booking.
• Keep updated records of microscope use by all users for charging.
• Perform weekly tests and calibrations as required on all electron microscopes.
• Effectively manage equipment time and communicate priorities amongst the various users.
• Attend meetings and conferences and keep up to date with literature in the CryoEM field
• Implement new tools, techniques, software, and hardware as they become available and as appropriate to user needs

Preferred Qualifications and Experience:

• Education: Ph.D. in physics, biophysics or materials science
• 5 years experience in the field of electron cryo-microscopy
• In-depth knowledge of all aspects of electron microscope hardware
• Knowledge of imaging and diffraction theory
• Expertise in sample preparation methods for cryoEM
• Working knowledge of Linux operating system
• Good working knowledge of scripting, computer programming and compiling of software
• Expertise in low-dose techniques and diffraction preferred
• Knowledge of image processing algorithms and proficiency in the use of common image processing packages preferred

Membrane protein biochemist at Mount Sinai School of Medicine - New York, NY

A position for a highly motivated membrane protein biochemist is available immediately in the department of Structural and Chemical Biology at Mount Sinai School of Medicine in New York (http://www.mssm.edu/departments-and-institutes/structural-and-chemical-biology/). This position will focus on the expression and purification of eukaryotic membrane protein targets for structure determination by electron cryo-microscopy of 2D crystals. The TEMIMPS consortium (see http://temimps.nysbc.org) is developing automated, high-throughput methods for 2D crystallization and structure determination of eukaryotic membrane protein targets of biological and medical relevance. This work will involve expression of eukaryotic membrane proteins in bacteria, yeast or insect cells. This work will also involve developing biochemical assays to characterize membrane protein stability and predisposition for 2D crystallization. The department of Structural and Chemical Biology at Mount Sinai School of Medicine is well equipped for all aspects of molecular biology and biochemistry of membrane proteins and has state-of-the-art equipment for Biophysics and Structural Biology. Candidates must hold a Ph.D. in Molecular Biology, Biochemistry, Biophysics, Structural Biology or an appropriately related field. A strong research experience in expression and purification of membrane proteins is a requisite. In addition, the candidate must have strong interpersonal, organizational and communication skills as the work will involve intimate collaboration with research groups at the New York Structural Biology Center and New York University. The salary will be commensurate with experience. Interested candidates should e-mail a cover letter, curriculum vitae and contact information for three professional referees to temimps@nysbc.org or to Dr. Iban Ubarretxena (iban.ubarretxena@mssm.edu).