Difference: TwodxAgrC (1 vs. 14)

Revision 1403 Apr 2014 - Main.NicolasCoudray

Changed:
<
<
META TOPICPARENT name="2DCrystallizationExp"
>
>
META TOPICPARENT name="TwoDCrystallizationGroup"
 

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X216 - Sparse matrix + charged lipids

Protein in 0.14% FC12: X216-Protein_Acquisition_AgrC.doc.

Sparse matrix + charged lipids tried: X216-AgrC.xlsx.

Results, X216

Aggregates or Aggregates+ vesicles for mostly of the PC lipids
Aggregates/clusters + lots of tiny “textured” vesicles (<150 nm) for most of the E.coli lipids and DOPG mixtures, or a bit with DOPC:DOPA.
Image selection: X216-AgrC-ImageSelection.pptx.

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)

Results, X162

Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

Image selection: X162-AgrC.xlsx.

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

Deleted:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340825569" name="X130CD-X131-ArgC.xlsx" path="X130CD-X131-ArgC.xlsx" size="602004" user="Main.NicolasCoudray" version="2"
Deleted:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482878" name="X216-AgrC.xlsx" path="X216-AgrC.xlsx" size="596323" user="Main.NicolasCoudray" version="1"
Deleted:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1344001157" name="X130_and_X131_ArgC.ppt" path="X130_and_X131_ArgC.ppt" size="7711232" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
 
META TOPICMOVED by="NicolasCoudray" date="1390912507" from="Main.TwodxArgC" to="Main.TwodxAgrC"

Revision 1328 Jan 2014 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X216 - Sparse matrix + charged lipids

Protein in 0.14% FC12: X216-Protein_Acquisition_AgrC.doc.

Sparse matrix + charged lipids tried: X216-AgrC.xlsx.

Results, X216

Aggregates or Aggregates+ vesicles for mostly of the PC lipids
Aggregates/clusters + lots of tiny “textured” vesicles (<150 nm) for most of the E.coli lipids and DOPG mixtures, or a bit with DOPC:DOPA.
Image selection: X216-AgrC-ImageSelection.pptx.

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)

Results, X162

Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

Image selection: X162-AgrC.xlsx.

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

Changed:
<
<
>
>
Deleted:
<
<

subsub level topic

a79 2
 
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340825569" name="X130CD-X131-ArgC.xlsx" path="X130CD-X131-ArgC.xlsx" size="602004" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482878" name="X216-AgrC.xlsx" path="X216-AgrC.xlsx" size="596323" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1344001157" name="X130_and_X131_ArgC.ppt" path="X130_and_X131_ArgC.ppt" size="7711232" user="Main.NicolasCoudray" version="1"
META TOPICMOVED by="NicolasCoudray" date="1390912507" from="Main.TwodxArgC" to="Main.TwodxAgrC"

Revision 1219 Jul 2013 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X216 - Sparse matrix + charged lipids

Protein in 0.14% FC12: X216-Protein_Acquisition_AgrC.doc.

Sparse matrix + charged lipids tried: X216-AgrC.xlsx.

Added:
>
>

Results, X216

Aggregates or Aggregates+ vesicles for mostly of the PC lipids
Aggregates/clusters + lots of tiny “textured” vesicles (<150 nm) for most of the E.coli lipids and DOPG mixtures, or a bit with DOPC:DOPA.
Image selection: X216-AgrC-ImageSelection.pptx.
 
Added:
>
>
 

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)

Results, X162

Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

Image selection: X162-AgrC.xlsx.

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340825569" name="X130CD-X131-ArgC.xlsx" path="X130CD-X131-ArgC.xlsx" size="602004" user="Main.NicolasCoudray" version="2"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482878" name="X216-AgrC.xlsx" path="X216-AgrC.xlsx" size="596323" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1344001157" name="X130_and_X131_ArgC.ppt" path="X130_and_X131_ArgC.ppt" size="7711232" user="Main.NicolasCoudray" version="1"
META TOPICMOVED by="NicolasCoudray" date="1390912507" from="Main.TwodxArgC" to="Main.TwodxAgrC"

Revision 1110 Jul 2013 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4
Added:
>
>

X216 - Sparse matrix + charged lipids

 
Added:
>
>		Protein in 0.14% FC12: X216-Protein_Acquisition_AgrC.doc.

Sparse matrix + charged lipids tried: X216-AgrC.xlsx.

 

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)

Results, X162

Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

Image selection: X162-AgrC.xlsx.

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482878" name="X216-AgrC.xlsx" path="X216-AgrC.xlsx" size="596323" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1344001157" name="X130_and_X131_ArgC.ppt" path="X130_and_X131_ArgC.ppt" size="7711232" user="Main.NicolasCoudray" version="1"
 
META TOPICMOVED by="NicolasCoudray" date="1390912507" from="Main.TwodxArgC" to="Main.TwodxAgrC"

Revision 1022 Jan 2013 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)

Results, X162

Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

Added:
>
>		Image selection: X162-AgrC.xlsx.
 
Added:
>
>
 

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

Changed:
<
<		 * X162-AgrC.xlsx: X162-AgrC.xlsx
>
>
Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820570" name="June2012-Protein_acquisition_form-ArgC.doc" path="June2012-Protein_acquisition_form-ArgC.doc" size="46592" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
Added:
>
>
META TOPICMOVED by="NicolasCoudray" date="1390912507" from="Main.TwodxArgC" to="Main.TwodxAgrC"
 

Revision 914 Jan 2013 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)
Added:
>
>

Results, X162

 
Added:
>
>		Mainly aggregates (also with biobeads tested by Marissa). Protein concentration may be higher than expected (gel may give some info).

 

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

* X162-AgrC.xlsx: X162-AgrC.xlsx

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
Changed:
<
<
META FILEATTACHMENT attachment="X162-AgrC.xlsx" attr="" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" stream="X162-AgrC.xlsx" user="Main.NicolasCoudray" version="1"
>
>

Revision 813 Dec 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - AgrC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4
Added:
>
>

X162

Protein in FC12. DMPC and DOPC in FC12, LPR 0.25 to 1, in 20ul dialysis buttons vs 1 buffer similar to the one in which the protein is (pH8)
 

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

Added:
>
>		 * X162-AgrC.xlsx: X162-AgrC.xlsx
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1373482869" name="X216-Protein_Acquisition_AgrC.doc" path="X216-Protein_Acquisition_AgrC.doc" size="47104" user="Main.NicolasCoudray" version="1"
 
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attachment="X162-AgrC.xlsx" attr="" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" stream="X162-AgrC.xlsx" user="Main.NicolasCoudray" version="1"

Revision 707 Dec 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"
Changed:
<
<

2dx - ArgC 2D-crystallization

>
>

2dx - AgrC 2D-crystallization

 Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attachment="X162-AgrC.xlsx" attr="" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" stream="X162-AgrC.xlsx" user="Main.NicolasCoudray" version="1"

Revision 607 Aug 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Image selection: X130_and_X131_ArgC.ppt. X130CD:
  • lots of vesicles (DMPC)
X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
Changed:
<
<
  • recommended lipid: DOPG (rather pH 7) look better
>
>
  • recommended lipid: DOPG (rather pH 7) look better (big vesicle at LPR 0.5: more images to be acquired)
 
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attachment="X162-AgrC.xlsx" attr="" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" stream="X162-AgrC.xlsx" user="Main.NicolasCoudray" version="1"

Revision 503 Aug 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
Changed:
<
<
  • buffer for dialysis: 20mM MES, 200mM NaCl?, 5mM MgCl2, 5 NaN3?
>
>
  • buffer for dialysis: 20mM MES, 200mM NaCl, 5mM MgCl2, 5 NaN3?
  See details: X130CD-X131-ArgC.xlsx.

Comments, July 2012

Added:
>
>		Image selection: X130_and_X131_ArgC.ppt.
 X130CD:
  • lots of vesicles (DMPC)
Added:
>
>		X131:
  • mostly vesicles are obtained
  • recommended lipid: DMPC (pH 6.0 or 7.0)
  • recommended lipid: DOPG (rather pH 7) look better
  • DMPC vesicles appear bigger when cyclodextrin robot has been used.
 
Added:
>
>
 

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"
Added:
>
>
META FILEATTACHMENT attachment="X162-AgrC.xlsx" attr="" comment="" date="1355417647" name="X162-AgrC.xlsx" path="X162-AgrC.xlsx" size="608615" stream="X162-AgrC.xlsx" user="Main.NicolasCoudray" version="1"

Revision 426 Jul 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees (and a few at 28-37 deg)
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl?, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

Added:
>
>

Comments, July 2012

X130CD:
  • lots of vesicles (DMPC)
 

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"

Revision 327 Jun 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc. Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
Changed:
<
<
  • 28 degrees
>
>
  • 28 degrees (and a few at 28-37 deg)
 
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl?, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
Changed:
<
<
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820579" name="X130CD-X131-ArgC.xlsx" path="X130CD-X131-ArgC.xlsx" size="597419" user="Main.NicolasCoudray" version="1"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1358880868" name="X162-AgrC-matrix.jpg" path="X162-AgrC-matrix.jpg" size="1171566" user="Main.NicolasCoudray" version="1"

Revision 227 Jun 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

Changed:
<
<

Name of the topic

Sublevel topic

>
>

X130CD-X131 - Lipid screening

Protein in 20mM Tris-ClH ph8: 100mM ClNa?: 2mM TCEP: 0.2% DM. Protein acquisition form: June2012-Protein_acquisition_form-ArgC.doc.
Added:
>
>		Conditions:
  • lipids solubilized in DM, like the protein (DMPC, E.coli, DOPG, EggPC?:PA)
  • LPR 0.25 to 1.5
  • pH6 and 7
  • 28 degrees
  • microdialysis plate (and MBCD robot, reaching Det:CD of 1:3 after 36 hours)
  • buffer for dialysis: 20mM MES, 200mM NaCl?, 5mM MgCl2, 5 NaN3?

See details: X130CD-X131-ArgC.xlsx.

 

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

Added:
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1374266136" name="X216-AgrC-ImageSelection.pptx" path="X216-AgrC-ImageSelection.pptx" size="8493234" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="" date="1340820579" name="X130CD-X131-ArgC.xlsx" path="X130CD-X131-ArgC.xlsx" size="597419" user="Main.NicolasCoudray" version="1"

Revision 119 Jun 2012 - Main.NicolasCoudray

 
META TOPICPARENT name="2DCrystallizationExp"

2dx - ArgC 2D-crystallization

Contents

Properties

  • Amino acid sequence:
MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIGISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

  • 430 amino acids
  • 49.8 kDa
  • pI ~ 5.28
  • 44 negatively charged residues
  • 38 positively charged residues
  • extinction coefficient: 38405
  • instability index: 38.13
  • alipathic index: 127.63
  • GRAVY: 0.510
  • TM helices: 6 (Phoebius)
  • amino acids in the TM domain: 138 (LPR(m/m) expected: 5-20 (or 10-160) ~ 0.1 to 0.3 in w/w (or 0.15 to 2.5); with LPR(m/m)=24>>LPR(w/w)=0.4

Name of the topic

Sublevel topic

subsub level topic

a79 2
  • Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Jun 2012

View topic  |  History: r14 < r13 < r12 < r11  |  More topic actions...
 
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