2dx - CD47 2D crystallization

Info

Human membrane proteins for 2D crystallization trial, CD47. It is stable during expression and purification from SF9 cells. It is also stable in all detergents tested in detergent stability assay. The N-terminal domain (soluble) of CD47 has been crystallized (3D) in 0.2 M MgCl2, 0.1 M Bis-Tris pH 5.5, 25% PEG3350 at 21 C. We will set up initial 2D crystallization based on this condition using different lipids.

Properties

• Amino acid sequence of H3:

MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDEAENLYFQSHHHHHHHHHHDYKDDDDK

• 349 amino acids
• 38.5 kDa
• pI ~ 6.35
• 31 negatively charged residues
• 27 positively charged residues
• extinction coefficient: 40000
• instability index: 25.69
• alipathic index: 111.72
• GRAVY: 0.375
• TM helices: 5
• amino acids in the TM domain: ~105
  • LPR 0.5(w/w) = 25 (m/m);

X241 - Lipids in 3dx buffer

Matrix details: X241-CD47.xlsx.

• Set ALLOWTOPICVIEW =

-- NicolasCoudray - 04 Oct 2013