Revision 803 Apr 2014 - Main.NicolasCoudray

Changed:

<
<

META TOPICPARENT	name="2DCrystallizationExp"

>
>

META TOPICPARENT	name="TwoDCrystallizationGroup"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP. X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2
2 mM MBCD added in all buffers after 1 week

Results, X146

See image selection: ralph_X146_X148.pptx.

Peak was close to void volume and broad. Iban suggests it's not a good sign
Many conditions crashed.

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1351259549" name="ralph_X146_X148.pptx" path="ralph_X146_X148.pptx" size="3876481" user="Main.RalphLasala" version="1"

Revision 726 Oct 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP. X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2
2 mM MBCD added in all buffers after 1 week

Results, X146

Added:

>
>

See image selection: ralph_X146_X148.pptx.

Peak was close to void volume and broad. Iban suggests it's not a good sign
Many conditions crashed.

Added:

>
>

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Deleted:

<
<

* ralph_X146_X148.pptx: ralph_X146_X148.pptx

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Changed:

<
<

META FILEATTACHMENT	attachment="ralph_X146_X148.pptx" attr="" comment="" date="1351259549" name="ralph_X146_X148.pptx" path="ralph_X146_X148.pptx" size="3876481" stream="ralph_X146_X148.pptx" user="Main.RalphLasala" version="1"

>
>

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1351259549" name="ralph_X146_X148.pptx" path="ralph_X146_X148.pptx" size="3876481" user="Main.RalphLasala" version="1"

Revision 626 Oct 2012 - Main.RalphLasala

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP. X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2
2 mM MBCD added in all buffers after 1 week

Results, X146

Peak was close to void volume and broad. Iban suggests it's not a good sign
Many conditions crashed.

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Added:

>
>

* ralph_X146_X148.pptx: ralph_X146_X148.pptx

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Added:

>
>

META FILEATTACHMENT	attachment="ralph_X146_X148.pptx" attr="" comment="" date="1351259549" name="ralph_X146_X148.pptx" path="ralph_X146_X148.pptx" size="3876481" stream="ralph_X146_X148.pptx" user="Main.RalphLasala" version="1"

Revision 516 Oct 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP. X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2
2 mM MBCD added in all buffers after 1 week

Added:

>
>

Results, X146

Peak was close to void volume and broad. Iban suggests it's not a good sign
Many conditions crashed.

Added:

>
>

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Added:

>
>

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Revision 401 Oct 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP. X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2

Added:

>
>

2 mM MBCD added in all buffers after 1 week

Deleted:

<
<

d53 1

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"

Deleted:

<
<

META FILEATTACHMENT	attachment="Protein_Acquisition_Form_.doc" attr="" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form .doc" size="63488" stream="Protein_Acquisition_Form .doc" user="Main.NicolasCoudray" version="2"

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Revision 324 Sep 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

X145CD - X146 - Buffer and lipid screening

Added:

>
>

Protein acquisition form: Protein_Acquisition_Form_.doc. Protein in 20mM Hepes, pH 7.8, 100mM NaCl^?, 0.05% DDM and 0.05mM TCEP.

X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2

d53 1

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Added:

>
>

META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form_.doc" size="63488" user="Main.NicolasCoudray" version="2"

META FILEATTACHMENT	attachment="Protein_Acquisition_Form_.doc" attr="" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form .doc" size="63488" stream="Protein_Acquisition_Form .doc" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Revision 220 Sep 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

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Sublevel topic

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X145CD - X146 - Buffer and lipid screening

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X145CD: in MBCD robot (detailed conditions: X145CD-GluTransp.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.35, 0.7, 1.4 and 2.2 (0.86 mg/ml protein)
1 ul of 4 M NaCl added (so final [ NaCl] ~ 225 mM)

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X146: in microdialysis plate (conditions: X146-Statistics_from_2dxTable.xlsx)

DMPC and E.coli in DDM and DOPC in C12E8
LPR 0.3, 0.6, 1.2 (and 2 for some conditions)
Buffers (all with 5 mM NaN3): selection based on k-mean applied on buffers used in the litterature with DMPC, E.coli or other lipids:
- for DMPC only:
  - 20 mM Tris pH8, 250 mM NaCl, 0 mM MgCl2
  - 20 mM TES pH7.5, 50 mM NaCl, 2 mM MgCl2
- for E.coli only:
  - 20 mM TES pH7.5, 100 mM NaCl, 5 mM MgCl2
  - 20 mM TES pH7.5, 200 mM NaCl, 0 mM MgCl2
- for all lipids:
  - 20 mM MES pH6, 100 mM NaCl, 20 mM MgCl2
  - 20 mM TES pH7.5, 280 mM NaCl, 5 mM MgCl2
  - 20 mM Tris pH8, 50 mM NaCl, 0 mM MgCl2

d53 1

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Added:

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META FILEATTACHMENT	attachment="Protein_Acquisition_Form_.doc" attr="" comment="" date="1348514045" name="Protein_Acquisition_Form_.doc" path="Protein_Acquisition_Form .doc" size="63488" stream="Protein_Acquisition_Form .doc" user="Main.NicolasCoudray" version="2"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166942" name="X146-Statistics_from_2dxTable.xlsx" path="X146-Statistics_from_2dxTable.xlsx" size="61485" user="Main.NicolasCoudray" version="1"
META FILEATTACHMENT	attr="" autoattached="1" comment="" date="1348166889" name="X145CD-GluTransp.xlsx" path="X145CD-GluTransp.xlsx" size="559187" user="Main.NicolasCoudray" version="2"

Revision 119 Sep 2012 - Main.NicolasCoudray

META TOPICPARENT	name="2DCrystallizationExp"

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Amino acid sequence:

MTPLDIQLLLTALASVLVLVALIVSRLKMHPLLALLVVSIGVGFATHMAPGSIVSHLLTGAGKTLGAVGVVIALGAMLGKILADAGVTEQVADVILKRTPDRMIPWAMMLVAFVIGIPMFFEVGLVIMLPLIFSVARKLESKARFKGSAYVYVGVPVISALAAMHGMVPPHPGPLTAIAVLKTSVGPTMLYGFLAAIPAMILGGPLYGMFISPRMNTRPDQALLDQFTLAEKADGQPRPGVMVGMLAALLPAILMLVHAVAEMLLPKGNALLEMASFLGNPLIAMLLGVLFAGASLVLARGGDAEQLRDALGKSLKPIASIIMIIAGGGAFQEMLTSAKVGDAIVHLTQQSAFPPLILGWLIAMLLSVSTGSATVGIVGAAGLLAPLAGADPSLNLPLLALSIGCGSLFFNYANHAGFWMVKESFGMTMGEATKTISVVQSIVAVVGLMVVLMLNAAITIG

461 amino acids
47.6 kDa
pI ~ 9.14
21 negatively charged residues
25 positively charged residues
extinction coefficient: 23950
instability index: 20.72
alipathic index: 131.43
GRAVY: 1.001
TM helices: 12 (Phoebius) or 10 (TMHMM)
amino acids in the TM domain: 255 or 227 (LPR(m/m) expected: 8-178 (16-63 peak ~30) ~ 0.13-2.92 w/w (0.26-1.04, peak at 0.49); with LPR(m/m)=24>>LPR(w/w)=0.4

Sublevel topic

d53 1

Set ALLOWTOPICVIEW =

-- NicolasCoudray - 19 Sep 2012

Difference: TwodxGluconateTransporter (1 vs. 8)

Revision 803 Apr 2014 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Results, X146

Revision 726 Oct 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Results, X146

Revision 626 Oct 2012 - Main.RalphLasala

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Results, X146

Revision 516 Oct 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Results, X146

Revision 401 Oct 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Revision 324 Sep 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

X145CD - X146 - Buffer and lipid screening

Revision 220 Sep 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Sublevel topic

X145CD - X146 - Buffer and lipid screening

Revision 119 Sep 2012 - Main.NicolasCoudray

2dx - gluconate transporter / permease; 2D-crystallization

Properties

Sublevel topic