Difference: TwodxmUMA (1 vs. 2)
Revision 203 Apr 2014 - Main.NicolasCoudray
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2dx - UMA mutant - 2d crystallizationMutant of UMA.X209 - Lipids at MSSMThe UMA triple mutant solubilized in detergent 1% DDM and then Ni-NTA purification was in DDM but finally gel filtration buffer contain detergent 0.2% DM and kept at 30C on June 6th, 2013 (sampled June 19th).Dialysis buffer contains 25mM Tris pH8.0, 150mM NaCl & 0.5mM TCEP. Condition: #1-5 was in DMPC and #6-10 was in DOPG LPR 0.2-1.0 X209, Results:Attached are a few pics of UMA. Mainly vesicles from LPR 0.6, aggregates below. Images: x209-mUMA.zip (A2--> A5 = DMPC LPR 0.4 to 1; A7 --> A11 = DOPG LPR 0.2 to 1)
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Revision 121 Jun 2013 - Main.NicolasCoudray
2dx - UMA mutant - 2d crystallizationMutant of UMA.X209 - Lipids at MSSMThe UMA triple mutant solubilized in detergent 1% DDM and then Ni-NTA purification was in DDM but finally gel filtration buffer contain detergent 0.2% DM and kept at 30C on June 6th, 2013 (sampled June 19th).Dialysis buffer contains 25mM Tris pH8.0, 150mM NaCl & 0.5mM TCEP. Condition: #1-5 was in DMPC and #6-10 was in DOPG LPR 0.2-1.0 X209, Results:Attached are a few pics of UMA. Mainly vesicles from LPR 0.6, aggregates below. Images: x209-mUMA.zip (A2--> A5 = DMPC LPR 0.4 to 1; A7 --> A11 = DOPG LPR 0.2 to 1)
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