Difference: WohlwendUse (1 vs. 8)

Revision 806 Jan 2014 - Main.BillRice

 
META TOPICPARENT name="HighPressFreezer"
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

Start up

Preparation

  • Lower pressure on standard 22PSI liquid nitrogen tank down to ~10 PSI by attaching pressure lowering device to vent outlet on tank
  • Attach carefully to WW, being particularly careful about connector on WW
Changed:
<
<
  • Open Zero Grade nitrogen gas valve. Pressure should be about 400 PSI (see photo)
>
>
  • Open nitrogen gas valve. Pressure should be about 400 PSI (see photo)
Added:
>
>
    • Zero grade not necessary. Use black tank.
 
  • Open side panel of machine and note current shot number
Pressure lowering device Full Setup N2 gas pressure
pressure_lower.jpg setup.jpg gaspressure.jpg

System Start

  • Turn machine on using large red switch on side
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Turn off "System Dry Up."
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
Deleted:
<
<
    • Oil pressure will increase to 100 bar
 
    • "Drive In" light will go out
  • Press green "Auto" button.
Added:
>
>
    • Oil pressure will increase to >100 bar
    • System usable when pressure >200 bar
 

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample
Added:
>
>

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Record shots taken in log
  • Once lamp stops flashing close nitrogen gas valve
 

Measuring temperature and pressure (traces to check performance)

See attached document:

Equipment needed

  • EasyScope USB Oscilloscope box and software
  • BNC to BNC cable
  • positive and negative cables plugged in to BNC converter
  • recording holder (for recording temperature)

Set up

Temperature

  • Plug green cable from grey box on top of WW into recording holder
  • Plug one end of black BNC cable into grep box, and other end into left position on EasyScope USB box
  • Remember to connect grey box to power outlet
  • Note: Green cable connector has a fine pin. Be careful to insert correctly and not to break the pin!
  • cable:
    easyscope3.jpg

Pressure

  • Plug black and red cables into pressure ports on WW, and plug combined BNC output into middle position on EasyScope box

Images of setup

easyscope1.jpg easyscope2.jpg

Computer

  • Plug EasyScope box into USB port of "old" lab laptop
  • Start EasyLogger software
  • Set up channel A and B as shown in screencaps below
Screencaps for EasySCope settings:

* Setup for ww channel A:
wohlwend_channelA_setup.PNG

* Setup for ww channel B:
wohlwend_channelB_setup.PNG

Recording

  • Set Channel B for trigger
  • Set trigger level as shown in screencap below
  • Put measuring holder (if recording temp) or actual holder (pressure only) in machine
  • After firing shot, should get results close to those shown below
  • May need to scroll screen sideways t be looking at appropriate time region

* Typical run on ww:
mar_22_2013_test_shot1.PNG

Deleted:
<
<

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Record shots taken in log
  • Once lamp stops flashing close nitrogen gas valve
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

* easyscope1:

* easyscope2:

* ww setup:

* pressurelower:

* gaspressure:

META FILEATTACHMENT attr="" autoattached="1" comment="Recording press and temp maintenance on WW HPF" date="1360104102" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" size="321475" user="Main.MarienaSilvestry" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="setup for ww channel a" date="1363978250" name="wohlwend_channelA_setup.PNG" path="wohlwend_channelA_setup.PNG" size="38695" user="Main.BillRice" version="1"
Changed:
<
<
META FILEATTACHMENT attachment="easyscope2.jpg" attr="" comment="easyscope2" date="1363986047" name="easyscope2.jpg" path="easyscope2.jpg" size="29364" stream="easyscope2.jpg" user="Main.BillRice" version="2"
>
>
META FILEATTACHMENT attr="" autoattached="1" comment="easyscope2" date="1363986047" name="easyscope2.jpg" path="easyscope2.jpg" size="29364" user="Main.BillRice" version="2"
 
META FILEATTACHMENT attr="" autoattached="1" comment="typical ron on ww" date="1363978448" name="mar_22_2013_test_shot1.PNG" path="mar_22_2013_test_shot1.PNG" size="20170" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="cable" date="1363986006" name="easyscope3.jpg" path="easyscope3.jpg" size="23366" user="Main.BillRice" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="ww setup" date="1363985260" name="setup.jpg" path="setup.jpg" size="29784" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="pressurelower" date="1363985628" name="pressure_lower.jpg" path="pressure_lower.jpg" size="14335" user="Main.BillRice" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="gaspressure" date="1363985324" name="gaspressure.jpg" path="gaspressure.jpg" size="35766" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="setup for ww channel b" date="1363978273" name="wohlwend_channelB_setup.PNG" path="wohlwend_channelB_setup.PNG" size="38663" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="easyscope1" date="1363986028" name="easyscope1.jpg" path="easyscope1.jpg" size="40690" user="Main.BillRice" version="2"

Revision 722 Mar 2013 - Main.BillRice

 
META TOPICPARENT name="HighPressFreezer"
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

Start up

Changed:
<
<
  • Open nitrogen gas valve
>
>

Preparation

Added:
>
>
  • Lower pressure on standard 22PSI liquid nitrogen tank down to ~10 PSI by attaching pressure lowering device to vent outlet on tank
  • Attach carefully to WW, being particularly careful about connector on WW
  • Open Zero Grade nitrogen gas valve. Pressure should be about 400 PSI (see photo)
  • Open side panel of machine and note current shot number
Pressure lowering device Full Setup N2 gas pressure
pressure_lower.jpg setup.jpg gaspressure.jpg

System Start

  • Turn machine on using large red switch on side
 
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Turn off "System Dry Up."
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample

Measuring temperature and pressure (traces to check performance)

See attached document:
Added:
>
>

Equipment needed

  • EasyScope USB Oscilloscope box and software
  • BNC to BNC cable
  • positive and negative cables plugged in to BNC converter
  • recording holder (for recording temperature)

Set up

Temperature

  • Plug green cable from grey box on top of WW into recording holder
  • Plug one end of black BNC cable into grep box, and other end into left position on EasyScope USB box
  • Remember to connect grey box to power outlet
  • Note: Green cable connector has a fine pin. Be careful to insert correctly and not to break the pin!
  • cable:
    easyscope3.jpg
 
Added:
>
>

Pressure

  • Plug black and red cables into pressure ports on WW, and plug combined BNC output into middle position on EasyScope box

Images of setup

easyscope1.jpg easyscope2.jpg

Computer

  • Plug EasyScope box into USB port of "old" lab laptop
  • Start EasyLogger software
  • Set up channel A and B as shown in screencaps below
Screencaps for EasySCope settings:

* Setup for ww channel A:
wohlwend_channelA_setup.PNG

* Setup for ww channel B:
wohlwend_channelB_setup.PNG

Recording

  • Set Channel B for trigger
  • Set trigger level as shown in screencap below
  • Put measuring holder (if recording temp) or actual holder (pressure only) in machine
  • After firing shot, should get results close to those shown below
  • May need to scroll screen sideways t be looking at appropriate time region

* Typical run on ww:
mar_22_2013_test_shot1.PNG

 

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
Added:
>
>
  • Record shots taken in log
 
  • Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Changed:
<
<
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" attr="" comment="Recording press and temp maintenance on WW HPF" date="1360104102" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How to record temperature and pressure traces on the Wohlwend HPF.docx" size="321475" stream="How to record temperature and pressure traces on the Wohlwend HPF.docx" user="Main.MarienaSilvestry" version="1"
>
>
Added:
>
>

* easyscope1:

* easyscope2:

* ww setup:

* pressurelower:

* gaspressure:

META FILEATTACHMENT attr="" autoattached="1" comment="Recording press and temp maintenance on WW HPF" date="1360104102" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" size="321475" user="Main.MarienaSilvestry" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="setup for ww channel a" date="1363978250" name="wohlwend_channelA_setup.PNG" path="wohlwend_channelA_setup.PNG" size="38695" user="Main.BillRice" version="1"
META FILEATTACHMENT attachment="easyscope2.jpg" attr="" comment="easyscope2" date="1363986047" name="easyscope2.jpg" path="easyscope2.jpg" size="29364" stream="easyscope2.jpg" user="Main.BillRice" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="typical ron on ww" date="1363978448" name="mar_22_2013_test_shot1.PNG" path="mar_22_2013_test_shot1.PNG" size="20170" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="cable" date="1363986006" name="easyscope3.jpg" path="easyscope3.jpg" size="23366" user="Main.BillRice" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="ww setup" date="1363985260" name="setup.jpg" path="setup.jpg" size="29784" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="pressurelower" date="1363985628" name="pressure_lower.jpg" path="pressure_lower.jpg" size="14335" user="Main.BillRice" version="2"
META FILEATTACHMENT attr="" autoattached="1" comment="gaspressure" date="1363985324" name="gaspressure.jpg" path="gaspressure.jpg" size="35766" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="setup for ww channel b" date="1363978273" name="wohlwend_channelB_setup.PNG" path="wohlwend_channelB_setup.PNG" size="38663" user="Main.BillRice" version="1"
META FILEATTACHMENT attr="" autoattached="1" comment="easyscope1" date="1363986028" name="easyscope1.jpg" path="easyscope1.jpg" size="40690" user="Main.BillRice" version="2"
 

Revision 605 Feb 2013 - Main.MarienaSilvestry

 
META TOPICPARENT name="HighPressFreezer"
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

Start up

  • Open nitrogen gas valve
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
Added:
>
>
  • Turn off "System Dry Up."
 
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample

Measuring temperature and pressure (traces to check performance)

See attached document:
Changed:
<
<		 * How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx: SOP on recording temperature and maintenance on the WW HPF
>
>
 

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Changed:
<
<
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" attr="" comment="SOP on recording temperature and maintenance on the WW HPF" date="1358544928" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How to record temperature and pressure traces on the Wohlwend HPF.docx" size="321108" stream="How to record temperature and pressure traces on the Wohlwend HPF.docx" user="Main.MarienaSilvestry" version="1"
>
>
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" attr="" comment="Recording press and temp maintenance on WW HPF" date="1360104102" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How to record temperature and pressure traces on the Wohlwend HPF.docx" size="321475" stream="How to record temperature and pressure traces on the Wohlwend HPF.docx" user="Main.MarienaSilvestry" version="1"
 

Revision 518 Jan 2013 - Main.MarienaSilvestry

 
META TOPICPARENT name="HighPressFreezer"
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

Start up

  • Open nitrogen gas valve
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample

Measuring temperature and pressure (traces to check performance)

Changed:
<
<		 *
>
>		 See attached document:
Added:
>
>		 * How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx: SOP on recording temperature and maintenance on the WW HPF
 

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Changed:
<
<
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.pdf" attr="" comment="SOP for taking traces on the Wolhwend HPF" date="1325201441" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.pdf" path="How to record temperature and pressure traces on the Wohlwend HPF.pdf" size="276714" stream="How to record temperature and pressure traces on the Wohlwend HPF.pdf" user="Main.MarienaSilvestry" version="1"
>
>
Added:
>
>
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" attr="" comment="SOP on recording temperature and maintenance on the WW HPF" date="1358544928" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.docx" path="How to record temperature and pressure traces on the Wohlwend HPF.docx" size="321108" stream="How to record temperature and pressure traces on the Wohlwend HPF.docx" user="Main.MarienaSilvestry" version="1"
 

Revision 429 Dec 2011 - Main.MarienaSilvestry

 
META TOPICPARENT name="HighPressFreezer"
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

Start up

  • Open nitrogen gas valve
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample
Added:
>
>

Measuring temperature and pressure (traces to check performance)

*
 

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Added:
>
>
META FILEATTACHMENT attachment="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.pdf" attr="" comment="SOP for taking traces on the Wolhwend HPF" date="1325201441" name="How_to_record_temperature_and_pressure_traces_on_the_Wohlwend_HPF.pdf" path="How to record temperature and pressure traces on the Wohlwend HPF.pdf" size="276714" stream="How to record temperature and pressure traces on the Wohlwend HPF.pdf" user="Main.MarienaSilvestry" version="1"

Revision 302 Oct 2009 - Main.KdDerr

 
META TOPICPARENT name="HighPressFreezer"
Changed:
<
<		Contents
>
>		Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM
Added:
>
>

Principles & Protocols

Protocols - Wohlwend

Jump to Principles

 
Deleted:
<
<

Wohlwend

 

Start up

  • Open nitrogen gas valve
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.

Use

  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample

Shut down

  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Revision 203 Oct 2007 - Main.KdDerr

 
META TOPICPARENT name="HighPressFreezer"
Contents

Wohlwend

Start up

Changed:
<
<		 *Open nitrogen gas valve *Press yellow "System Dry Up" button. Run gas through for 30 minutes. *Press green "On/Off" button *Press green "Nitrogen" button *Open liquid nitrogen tank *Wait until red "Nitrogen Level" light goes out *Press yellow "Drive in" button. *yellow light will start flashing *"Piston Down" red lamp will go on *Oil pressure will increase to 100 bar *"Drive In" light will go out *Press green "Auto" button.
>
>
  • Open nitrogen gas valve
  • Press yellow "System Dry Up" button. Run gas through for 30 minutes.
  • Press green "On/Off" button
  • Press green "Nitrogen" button
  • Open liquid nitrogen tank
  • Wait until red "Nitrogen Level" light goes out
  • Press yellow "Drive in" button.
    • Yellow light will start flashing
    • "Piston Down" red lamp will go on
    • Oil pressure will increase to 100 bar
    • "Drive In" light will go out
  • Press green "Auto" button.
 

Use

Changed:
<
<		 *Insert holder and safety pin *Green "Jet Ready" lamp should light *Press yellow "Jet Auto" button *Wait for yellow "Jet Over" lamp *Pull pin and remove sample
>
>
  • Insert holder and safety pin
  • Green "Jet Ready" lamp should light
  • Press yellow "Jet Auto" button
  • Wait for yellow "Jet Over" lamp
  • Pull pin and remove sample
 

Shut down

Changed:
<
<		 *Close liquid nitrogen tank *Press green "On/Off" Button *Press yellow "System Dry Up" button *Light will blink for 10 hours while system drys *Once lamp stops flashing close nitrogen gas valve
>
>
  • Close liquid nitrogen tank
  • Press green "On/Off" Button
  • Press yellow "System Dry Up" button * Light will blink for 10 hours while system drys
  • Once lamp stops flashing close nitrogen gas valve
 
  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

Revision 103 Oct 2007 - Main.KdDerr

 
META TOPICPARENT name="HighPressFreezer"
Contents

Wohlwend

Start up

*Open nitrogen gas valve *Press yellow "System Dry Up" button. Run gas through for 30 minutes. *Press green "On/Off" button *Press green "Nitrogen" button *Open liquid nitrogen tank *Wait until red "Nitrogen Level" light goes out *Press yellow "Drive in" button. *yellow light will start flashing *"Piston Down" red lamp will go on *Oil pressure will increase to 100 bar *"Drive In" light will go out *Press green "Auto" button.

Use

*Insert holder and safety pin *Green "Jet Ready" lamp should light *Press yellow "Jet Auto" button *Wait for yellow "Jet Over" lamp *Pull pin and remove sample

Shut down

*Close liquid nitrogen tank *Press green "On/Off" Button *Press yellow "System Dry Up" button *Light will blink for 10 hours while system drys *Once lamp stops flashing close nitrogen gas valve

  • Set ALLOWTOPICVIEW =

-- KdDerr - 03 Oct 2007

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