25 Mar 2005 - 28 Mar 2005 - Werner2005 Jan 6 Milton Werner overnights, weekendsThe 60 kD complex of a cell death and cell growth regulator, PEA-15, bound to ERK2. The complex has had trosy data collected at 800 MHz on both side of the complex. We are not committed to full backbone assignment of ERK2 (42 kD) and need 900 MHz for optimal sensitivity of the TROSY-based correlation methods. We are also screening 67 kD samples of a constitutively activated death receptor. The receptor's activity was validated by stable transfection into HEK293 cells and analysis of the resulting complex using immunoprecipitation. GM supported Time allocation comm. approved Mar 1 ALLOCATED
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