| META TOPICPARENT | name="ReQ900" |
|---|
| 2004 Dec 16 | NYSBC NMR Facility | Those not otherwise used | Optimization of ultra high field procedures | - |
| 2005 Jan 6 | Milton Werner | overnights, weekends | The 60 kD complex of a cell death and cell growth regulator, PEA-15, bound to ERK2. The complex has had trosy data collected at 800 MHz on both side of the complex. We are not committed to full backbone assignment of ERK2 (42 kD) and need 900 MHz for optimal sensitivity of the TROSY-based correlation methods. We are also screening 67 kD samples of a constitutively activated death receptor. The receptor's activity was validated by stable transfection into HEK293 cells and analysis of the resulting complex using immunoprecipitation. GM supported | Time allocation comm. approved Mar 1 ALLOCATED x 1, idle |
| 24 jan 2005 | McDermott? | I would like to request approximately 1 week | to characterize the probe and also make measurements on a promising intrinsic membrane protein (for which we have lower field data). | Approved, and to be scheduled when there is probe availability and testing. Waiting next cryoprobe outage and solid probe availability |
| 2 Mar 05 | Guy Montelione | 3-4 days | i have a project i am working on ... that is not related to the nesg project (so does not fall under that access mechanism)
we want to measure some rdc's at 800 or 900 mhz using a bruker system (we have all the pulse sequences worked out on 500 mhz bruker system)
would it be possible to get 4 days of 800 or 900 time to make these measurements; i guess i am in the 900 user group, though i have never tried to access the nydbc 900 before - but 800 will be enough)
our experiments should work with or without cryoprobe
we would send swapna gurla to do the measurements; i think she is certified for using the nysbc machines
guy | Approved Mar 16 allocated x 1 idle |
| Mar 23 | MingZhou? | 1 week | I am writing to request formally about 1 week instrument on the 900 system for intermolecular NOEs data collection for a protein/ligand complex (25 kDa). | allocated x 2, repeating |
| May 31 | JoelButterwick (Col.U/Palmer) | 1 week | On/off-resonance rotating frame relaxation experiments on a 20 kD protein. | Experiments with strong rf pulses cannot be performed easily on spectrometers equipped with cryoprobes. We have collected data at 500 MHz and would like data at either 800 or 900 MHz, but all the 800's have cryoprobes! | allocated, repeating, not available for cryoprobe |
| Nov 21 2005 | Dongsheng Liu | 2 days | Kinase domain, 2D and 3D | allocated -MG repeating |
| Nov 29 2005 | Ahn Tuan Phan | 12/13 through 12/16 | I would like to have some time on the 900 for a 3D C13edited-NOESY on a very diluted sample (~0.2-0.3mM). | allocated -MG |
| Dec 14 2005 | Dongsheng Liu | 1 day or overnight | TROSY on ligated csk | allocated -MG |
| Jan 17 2006 | SergeIlinSchneider | Jan 25-Jan 31 - week would be perfect | various 3D experiments on apo and ligand complex (20kDa) | allocated-MG |
| Feb 1 2006 | RonSeidel? | 1 day Tuesday 7th - Wed 8th | Check 35 kDa protein (N15 labeled) | allocated-MG |
| Feb 1 2006 | RonSeidel? | 3-4 days, starting from Feb 14th | Check 35 kDa protein (DN labeled) | Allocated-MG |
| Feb 13 2006 | SergeIlinSchneider | Feb 17-21 | noesy type 3D experiments on yng1-phd domain in apo and complex form (18kDa) | Allocated-MG |
| Mar 15 2006 | Dongsheng Liu | 2 days | Csk with ligand | allocated-MG |
| Mar 29 2006 | Thomas Szyperski | 7 d | I would like to request one week of 900 MHz time to acquire data | for a 45 kDa protein-DNA complex. | allocated - MG |
| Apr 19 2006 | VesselinMiloushev | 4-7 d, starting May 8th or later | I would like to collect 2D-3D NOESY spectra | (A) 12kD alpha helical protein. (B) 22kD dimer-monomer sample. | allocated - MG |
| May 4 2006 | Milton Werner | 6 d, starting May 10th or thereabouts | 25 kD complex of intact transcription factor and 13C/15N peptide--peptide concentration limiting (0.2 mM) | allocated -MG |
| May 30 2006 | David Eliezer | 4-7 d, starting July 12 or later | 3D and/or RDC experiments on 36kd homodimer and disordered mutants | allocated -MG |
| Jun 16 2006 | Dongsheng Liu/David Cowburn | 2 d, Jun 19 or later (canceled) | Csk kinase | allocated-MG |
| Jul 10 2006 | Dongsheng Liu/David Cowburn | 5 d or less, | Csk kinase, the sooner, the better | allocated-MG
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Jul 172006
| RonnieGhose | 7 days
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| allocated-MG
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Aug 4 2006
| Lei Zeng/Ming-Ming Zhou | 4 days
|
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| allocated-MG
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Aug 8 2006
| MSSM/Lei Zeng | 7 days
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| allocated-MG |
| Jul 28 2006 | MarkGirvin | 2 x 3 days, starting any time after August 14th | 1HN relaxation measurements on spin-labeled membrane protein in bicelles | Need to do 2 samples: spin-labeled and control, for 2.5-3 days each. Could be back-to-back (5-6 days total) | allocated-MG |
| Aug 21 2006 | JoelButterwick | 2 days | TROSY experiments to characterize a membrane protein in detergent micelles and bicelle mixtures | allocated-MG |
Aug 28 2006
| MSSM/Lei Zeng | 5 days
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| allocated-MG
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