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2dx Overview Contents Protocols Computing... crash total computer: ssh from another machine sudo init 3 sudo kill 9 whatever Xorg sudo init ...
Overview Detailed Syllabus: Jan 11 : Introduction SEMC tour Welcome to the Simons Electron Microscopy Center and explanation of the course. A tour of NYSBC and ...
Contents Fei Helios Instructions Sample prep Samples come in resin blocks Trim block size These generally need to be trimmed to ~1cm height so they will ...
CEMFacGroup Page CEM Administration Page 2014 Archive of CemMaintenance 2013 Archive of CemMaintenance CEM Equipment Mainenance Maintenance Checklist Monthly ...
Contents See general guidelines for metal shadowing here. Rotary shadowing using the Edwards Auto 306 Evaporator General setup Make sure gloves are worn at ...
Contents Ongoing issues Tecnai New apertures installed March 6 2015 Objective: 100 um, 50 um, 50 um, 40 um condenser: 150 um, 100 um, 70 um, 50 um BUT third ...
CEMUserhandbook ver1.pdf: CEM User Handbook version 1.00 EM User Handbook Note: Policies may have been updated since this handbook has been completed so please contact ...
Cryosectioning notes from Chyongere Hirsch (Wadsworth Center Albany,NY)24 Mar 05 Grids 200 mesh Quantifoil for tomography Glow discharge Carbon coat Gold ...
Detergent Removal with Cyclodextrine Experiments Contents Overview, references... Experiments 2011, August 25th Tuesday's meeting comment CD Yiip in microdialysis ...
Contents Standard Operating Procedure Coating of resin embedded samples attached to stubs 1 Turn on machine (switch on back) 1 Open chamber 1 Put sample ...
Contents Assessment of FIB instruments Visit to NIH Overall The instrument at the NIH is a FEI Quanta SEM with a focused ion beam. The focused ion beam is the usual ...
Return to the Cryo EM Website at http://www.nysbc.org/facilities/CEM Taking a film image Insert small screen adjust brightness until the exposure meter reads ...
Tuesday Group Meeting Reports 03/05/2013 UMA, MCMJR1 (Iban) 5 mutations done bt Bryan (as in the published paper). Expression ok. Will be scaled up at MSSM ...
Room 118 LogBook; experiments, debugging and updating... Manual use of 1230 1. Check the robot is not running (Leginon would appear and run on the main screen ...
JEOL 2100 Instructions Visit the Cryo EM Web site at http://www.nysbc.org/facilities/CEM K2 Camera Regular Maintenance Anneal cycle should be run weekly ...
JEOL 3200FSC The JEOL 3200FSC is a state of the art instrument that operates with an accelerating voltage of 300kV. It is equipped with a Schottky field emission gun ...
JEOL 3200 Microscope Parameters wavelengths 300kV: 0.01969 A objective lenses f 4.4 mm Cs 4.1 mm Chromatic aberration 3.4 mm Aperture ...
Contents JEOL 3200 training 3200 training notes.doc: DLS notes on 3200 training Cryogen fill Chuck dewar Fill in morning and last thing at night ...
Contents Metal Evaporation Pick appropiate metal diameter Pick LT or HT on Selector LT 10V 90 Amp HT 30V 30 Amp Amp meter reads ...
Tests of the micro dialysis plates performances Reference See MicroDialysisPlatesTool for an overview and information about the protocol. Experiments; test of the ...
Micro dialysis plates Overview (Martin's archive July 2010) Design An advantage of 2D crystallization relative to 3D crystallization is the use of relatively low ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Microtome Conversion Remove Cryosectioning unit Bake ...
Notes For 800conv Pulse Calibrations For pulse calibrations click on the following link: shapepulse 800CNV 44918 0025.xls: PowerMeasurements800conv Power Measurements ...
Contents Internal features Fitting a single Kd to multiple shift titration data NmrTitration 1 Demo script LIBRARY:matlab/lib1/titrationscript.html or NmrTitration ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Solid Carbon Film Jump to Principles Using Mica (Best ...
TLC of lipids and detergents – Martin's procedure Materials TLC Silica gel 60 W F254 These instructions are relevant for these plates only. They can be ordered ...
Contents Sample Insertion InsertRoomTemperatureSample InsertCryoSample Tecnai F20 Problems Instructions to Retrieve CCDBlankEnable Film Jam Access ...
(15.4; 688.1; 0.1) Robot Manuals (leave this at the top) Controller manual RCX142 e.pdf: rcx142 manual (this is most useful) RCX143 144 e 0322 v1.02.pdf ...
Return to Cryo EM web page at http://www.nysbc.org/facilities/CEM Principles Protocols Protocols Edwards 306 Vacuum Evaporator Jump to Principles Carbon ...
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