Difference: AllHoursAccessCryoEM (1 vs. 19)

Revision 1918 Aug 2009 - Main.KdDerr

 
META TOPICPARENT name="CemfacGroup"
Added:
>
>

NYSBC CEMfac all hours access

 
Deleted:
<
<		NYSBC CEMfac all hours access
Interested in EM? Using CEMfac? Principles & Protocols CEMfac Equipment Seminars & Courses
CEMfac Home? CEM Introduction Microscope Schedule NYSBC Directory Cryo-EM public website
 
Contents

How to acquire authorization for independent use of microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, several requirements need to be met before independent use of the instruments is approved.

1). Meet requirements for 24 hour access to the NYSBC .

2). Special training on EM operation must be completed. Training will only apply to individual instruments, as each has slightly different requirements. The topics to be covered are listed below.

3). User will be given an opportunity to practice independent instrument use during normal working hours. When ready, user will then take an operational test with EM staff to establish final authorization for independent use.

Session 1: Absolute Basics of operation

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

Session 2: General Setup of microscope

  1. How to align the optical axis of the instrument
    1. gun tilt, gun shift, voltage center
  2. How to align the spot sizes
  3. How to align the pivot points
  4. How to correct for condenser astigmatism
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Session 3: Advanced Setup of Microscope

  1. How to set up the low dose kit of the microscope.
    1. search mode using the fast scan camera and/or binoculars i focus mode at a suitably high magnification
    2. imaging on CCD or film
  2. How to select appropriate electron dose and exposure time
  3. SerialEM (optional)
    1. set up SerialEM in low-dose mode.
    2. set up and collect a tilt series.
    3. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  4. Slow Scan CCD camera operation
    1. Flatfield correction
    2. Gain adjustments and features EM menu
    3. Binning of images
  5. Refresh rate of the fast scan camera.
  6. How user Film (optional)
  7. How to view and record electron diffraction patterns (e.g. for checking for vitreous ice)
  8. how to safely leave the microscope at the end of the day

Session 4: Sample Insertion and Removal

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Session 5: Emergency Procedures

  1. What to do when software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

META TOPICMOVED by="DavidCowburn" date="1209473320" from="Main.AllHourAccessCEM" to="Main.AllHoursAccessCryoEM"

Revision 1812 Feb 2009 - Main.KakoliMitra

 
META TOPICPARENT name="CemfacGroup"
Added:
>
>
NYSBC CEMfac all hours access
Interested in EM? Using CEMfac? Principles & Protocols CEMfac Equipment Seminars & Courses
CEMfac Home? CEM Introduction Microscope Schedule NYSBC Directory Cryo-EM public website
 Contents

How to acquire authorization for independent use of microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, several requirements need to be met before independent use of the instruments is approved.

1). Meet requirements for 24 hour access to the NYSBC .

2). Special training on EM operation must be completed. Training will only apply to individual instruments, as each has slightly different requirements. The topics to be covered are listed below.

3). User will be given an opportunity to practice independent instrument use during normal working hours. When ready, user will then take an operational test with EM staff to establish final authorization for independent use.

Session 1: Absolute Basics of operation

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

Session 2: General Setup of microscope

  1. How to align the optical axis of the instrument
    1. gun tilt, gun shift, voltage center
  2. How to align the spot sizes
  3. How to align the pivot points
  4. How to correct for condenser astigmatism
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Session 3: Advanced Setup of Microscope

  1. How to set up the low dose kit of the microscope.
    1. search mode using the fast scan camera and/or binoculars i focus mode at a suitably high magnification
    2. imaging on CCD or film
  2. How to select appropriate electron dose and exposure time
  3. SerialEM (optional)
    1. set up SerialEM in low-dose mode.
    2. set up and collect a tilt series.
    3. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  4. Slow Scan CCD camera operation
    1. Flatfield correction
    2. Gain adjustments and features EM menu
    3. Binning of images
  5. Refresh rate of the fast scan camera.
  6. How user Film (optional)
  7. How to view and record electron diffraction patterns (e.g. for checking for vitreous ice)
  8. how to safely leave the microscope at the end of the day

Session 4: Sample Insertion and Removal

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Session 5: Emergency Procedures

  1. What to do when software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

META TOPICMOVED by="DavidCowburn" date="1209473320" from="Main.AllHourAccessCEM" to="Main.AllHoursAccessCryoEM"

Revision 1729 Apr 2008 - Main.DavidCowburn

 
META TOPICPARENT name="CemfacGroup"
Contents

How to acquire authorization for independent use of microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, several requirements need to be met before independent use of the instruments is approved.

1). Meet requirements for 24 hour access to the NYSBC .

2). Special training on EM operation must be completed. Training will only apply to individual instruments, as each has slightly different requirements. The topics to be covered are listed below.

3). User will be given an opportunity to practice independent instrument use during normal working hours. When ready, user will then take an operational test with EM staff to establish final authorization for independent use.

Session 1: Absolute Basics of operation

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

Session 2: General Setup of microscope

  1. How to align the optical axis of the instrument
    1. gun tilt, gun shift, voltage center
  2. How to align the spot sizes
  3. How to align the pivot points
  4. How to correct for condenser astigmatism
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Session 3: Advanced Setup of Microscope

  1. How to set up the low dose kit of the microscope.
    1. search mode using the fast scan camera and/or binoculars i focus mode at a suitably high magnification
    2. imaging on CCD or film
  2. How to select appropriate electron dose and exposure time
  3. SerialEM (optional)
    1. set up SerialEM in low-dose mode.
    2. set up and collect a tilt series.
    3. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  4. Slow Scan CCD camera operation
    1. Flatfield correction
    2. Gain adjustments and features EM menu
    3. Binning of images
  5. Refresh rate of the fast scan camera.
  6. How user Film (optional)
  7. How to view and record electron diffraction patterns (e.g. for checking for vitreous ice)
  8. how to safely leave the microscope at the end of the day

Session 4: Sample Insertion and Removal

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Session 5: Emergency Procedures

  1. What to do when software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Changed:
<
<
META TOPICMOVED by="DavidCowburn" date="1205585356" from="Main.AllHourAccess" to="Main.AllHourAccessCEM"
>
>
META TOPICMOVED by="DavidCowburn" date="1209473320" from="Main.AllHourAccessCEM" to="Main.AllHoursAccessCryoEM"
 

Revision 1615 Mar 2008 - Main.DavidStokes

 
META TOPICPARENT name="CemfacGroup"
Contents

How to acquire authorization for independent use of microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, several requirements need to be met before independent use of the instruments is approved.

1). Meet requirements for 24 hour access to the NYSBC .

2). Special training on EM operation must be completed. Training will only apply to individual instruments, as each has slightly different requirements. The topics to be covered are listed below.

3). User will be given an opportunity to practice independent instrument use during normal working hours. When ready, user will then take an operational test with EM staff to establish final authorization for independent use.

Session 1: Absolute Basics of operation

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture
Changed:
<
<

General Setup of microscope

>
>

Session 2: General Setup of microscope

 
  1. How to align the optical axis of the instrument
    1. gun tilt, gun shift, voltage center
  2. How to align the spot sizes
  3. How to align the pivot points
  4. How to correct for condenser astigmatism
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism
Changed:
<
<

Advanced Setup of Microscope

>
>

Session 3: Advanced Setup of Microscope

 
  1. How to set up the low dose kit of the microscope.
    1. search mode using the fast scan camera and/or binoculars i focus mode at a suitably high magnification
    2. imaging on CCD or film
  2. How to select appropriate electron dose and exposure time
  3. SerialEM (optional)
    1. set up SerialEM in low-dose mode.
    2. set up and collect a tilt series.
    3. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  4. Slow Scan CCD camera operation
    1. Flatfield correction
    2. Gain adjustments and features EM menu
    3. Binning of images
  5. Refresh rate of the fast scan camera.
  6. How user Film (optional)
  7. How to view and record electron diffraction patterns (e.g. for checking for vitreous ice)
  8. how to safely leave the microscope at the end of the day
Changed:
<
<

Sample Insertion and Removal

>
>

Session 4: Sample Insertion and Removal

 
  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
Changed:
<
<

Emergency Procedures

>
>

Session 5: Emergency Procedures

 
  1. What to do when software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

META TOPICMOVED by="DavidCowburn" date="1205585356" from="Main.AllHourAccess" to="Main.AllHourAccessCEM"

Revision 1515 Mar 2008 - Main.DavidStokes

 
META TOPICPARENT name="CemfacGroup"
Contents
Changed:
<
<

AllHourAccessCEM?

>
>

How to acquire authorization for independent use of microscopes at any hour of the day

Deleted:
<
<

How to use the microscopes at any hour of the day

 
Changed:
<
<		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, *the user must have met all the requirements for 24 hour access to the NYSBC*. The topics to be covered are the following:
>
>		The electron microscopes are in principle available for ~20 hours per day for data collection. However, several requirements need to be met before independent use of the instruments is approved.
 
Changed:
<
<

Absolute Basics of operation

>
>		1). Meet requirements for 24 hour access to the NYSBC .
Deleted:
<
<		The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
 
Added:
>
>		2). Special training on EM operation must be completed. Training will only apply to individual instruments, as each has slightly different requirements. The topics to be covered are listed below.

3). User will be given an opportunity to practice independent instrument use during normal working hours. When ready, user will then take an operational test with EM staff to establish final authorization for independent use.

Session 1: Absolute Basics of operation

 
  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

General Setup of microscope

Deleted:
<
<		Then the trainee will need to learn the following:
 
  1. How to align the optical axis of the instrument
Changed:
<
<
  1. How to correct for condenser astigmatism
>
>
    1. gun tilt, gun shift, voltage center
 
  1. How to align the spot sizes
  2. How to align the pivot points
Added:
>
>
  1. How to correct for condenser astigmatism
 
  1. How to introduce an objective aperture.
  2. How to correct for objective astigmatism

Advanced Setup of Microscope

Deleted:
<
<		After these alignments, the trainee will need to learn
 
Changed:
<
<
  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
  2. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  3. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Binning of the CCD images. Film settings.
>
>
  1. How to set up the low dose kit of the microscope.
    1. search mode using the fast scan camera and/or binoculars i focus mode at a suitably high magnification
Added:
>
>
    1. imaging on CCD or film
  2. How to select appropriate electron dose and exposure time
  3. SerialEM (optional)
    1. set up SerialEM in low-dose mode.
    2. set up and collect a tilt series.
    3. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  4. Slow Scan CCD camera operation
    1. Flatfield correction
    2. Gain adjustments and features EM menu
    3. Binning of images
 
  1. Refresh rate of the fast scan camera.
Changed:
<
<
  1. Diffraction mode. How to get electron diffraction patterns
>
>
  1. How user Film (optional)
Added:
>
>
  1. How to view and record electron diffraction patterns (e.g. for checking for vitreous ice)
 
  1. how to safely leave the microscope at the end of the day

Sample Insertion and Removal

Deleted:
<
<		After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.
 
  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Deleted:
<
<		Once these topics have been taught, information about common emergency procedures and precautions will be given, such as
 
Changed:
<
<
  1. What to do when the software crashes
>
>
  1. What to do when software crashes
 
  1. What to do in case the vacuum breaks
  2. What to do if the beam shuts down
  3. What to do if the lenses are not responding
  4. What to do if there is water leaking
  5. Precautions to be followed with liquid nitrogen
  6. Precautions to be followed with liquid ethane
  7. Etiquette of the electron microscopy facility
Deleted:
<
<		Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.
 
  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Added:
>
>
 
META TOPICMOVED by="DavidCowburn" date="1205585356" from="Main.AllHourAccess" to="Main.AllHourAccessCEM"

Revision 1415 Mar 2008 - Main.DavidCowburn

 
META TOPICPARENT name="CemfacGroup"
Contents
Changed:
<
<

AllHourAccess?

>
>

AllHourAccessCEM?

 

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, *the user must have met all the requirements for 24 hour access to the NYSBC*. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  1. How to align the optical axis of the instrument
  2. How to correct for condenser astigmatism
  3. How to align the spot sizes
  4. How to align the pivot points
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
  2. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  3. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Binning of the CCD images. Film settings.
  4. Refresh rate of the fast scan camera.
  5. Diffraction mode. How to get electron diffraction patterns
  6. how to safely leave the microscope at the end of the day

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as

  1. What to do when the software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Added:
>
>
META TOPICMOVED by="DavidCowburn" date="1205585356" from="Main.AllHourAccess" to="Main.AllHourAccessCEM"
 

Revision 1315 Mar 2008 - Main.DavidCowburn

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

Changed:
<
<		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:
>
>		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, *the user must have met all the requirements for 24 hour access to the NYSBC*. The topics to be covered are the following:
 

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  1. How to align the optical axis of the instrument
  2. How to correct for condenser astigmatism
  3. How to align the spot sizes
  4. How to align the pivot points
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
  2. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  3. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Binning of the CCD images. Film settings.
  4. Refresh rate of the fast scan camera.
  5. Diffraction mode. How to get electron diffraction patterns
  6. how to safely leave the microscope at the end of the day

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as

  1. What to do when the software crashes
  2. What to do in case the vacuum breaks
  3. What to do if the beam shuts down
  4. What to do if the lenses are not responding
  5. What to do if there is water leaking
  6. Precautions to be followed with liquid nitrogen
  7. Precautions to be followed with liquid ethane
  8. Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 1214 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  1. How to align the optical axis of the instrument
  2. How to correct for condenser astigmatism
  3. How to align the spot sizes
  4. How to align the pivot points
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
  2. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
Changed:
<
<
  1. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
>
>
  1. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Binning of the CCD images. Film settings.
 
  1. Refresh rate of the fast scan camera.
  2. Diffraction mode. How to get electron diffraction patterns
Changed:
<
<
  1. how to safely leave the microscope at the end of the day.
>
>
  1. how to safely leave the microscope at the end of the day
 

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as
Added:
>
>
  1. What to do when the software crashes
 
  1. What to do in case the vacuum breaks
  2. What to do if the beam shuts down
  3. What to do if the lenses are not responding
  4. What to do if there is water leaking
  5. Precautions to be followed with liquid nitrogen
  6. Precautions to be followed with liquid ethane
  7. Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 1114 Mar 2008 - Main.BillRice

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
  2. How to adjust the eucentric height of the specimen
  3. How to adjust the intensity of the beam
  4. How to focus the image
  5. How to change magnification
  6. How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  1. How to align the optical axis of the instrument
  2. How to correct for condenser astigmatism
  3. How to align the spot sizes
  4. How to align the pivot points
  5. How to introduce an objective aperture.
  6. How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
  2. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  3. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
  4. Refresh rate of the fast scan camera.
  5. Diffraction mode. How to get electron diffraction patterns
Added:
>
>
  1. how to safely leave the microscope at the end of the day.
 

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
  2. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  3. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  4. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as

  1. What to do in case the vacuum breaks
  2. What to do if the beam shuts down
  3. What to do if the lenses are not responding
  4. What to do if there is water leaking
  5. Precautions to be followed with liquid nitrogen
  6. Precautions to be followed with liquid ethane
  7. Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 1014 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
Changed:
<
<
  1. How to get an electron beam
>
>
  1. How to get an electron beam
Added:
>
>
  1. How to adjust the eucentric height of the specimen
  2. How to adjust the intensity of the beam
  3. How to focus the image
  4. How to change magnification
  5. How to introduce and center a condenser aperture
 
Deleted:
<
<
  1. How to adjust the eucentric height of the specimen

  1. How to adjust the intensity of the beam

  1. How to focus the image

  1. How to change magnification

  1. How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:
Changed:
<
<
  1. How to align the optical axis of the instrument
>
>
  1. How to align the optical axis of the instrument
Added:
>
>
  1. How to correct for condenser astigmatism
  2. How to align the spot sizes
  3. How to align the pivot points
  4. How to introduce an objective aperture.
  5. How to correct for objective astigmatism
 
Deleted:
<
<		 b. How to correct for condenser astigmatism

c. How to align the spot sizes

d. How to align the pivot points

e. How to introduce an objective aperture.

f. How to correct for objective astigmatism

 

Advanced Setup of Microscope

After these alignments, the trainee will need to learn
Changed:
<
<
  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
>
>
  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
Added:
>
>
  1. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
  2. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
  3. Refresh rate of the fast scan camera.
  4. Diffraction mode. How to get electron diffraction patterns
 
Deleted:
<
<		 b. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

c. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.

d. Refresh rate of the fast scan camera.

e. Diffraction mode. How to get electron diffraction patterns

 

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

Changed:
<
<
  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
>
>
  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
Added:
>
>
  1. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
  2. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
  3. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
 
Deleted:
<
<		 b. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

c. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

d. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

 

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as
Changed:
<
<
  1. What to do in case the vacuum breaks

b. What to do if the beam shuts down

c. What to do if the lenses are not responding

d. What to do if there is water leaking

>
>
  1. What to do in case the vacuum breaks
  2. What to do if the beam shuts down
  3. What to do if the lenses are not responding
  4. What to do if there is water leaking
  5. Precautions to be followed with liquid nitrogen
  6. Precautions to be followed with liquid ethane
  7. Etiquette of the electron microscopy facility
Deleted:
<
<		 e. Precautions to be followed with liquid nitrogen

f. Precautions to be followed with liquid ethane

g. Etiquette of the electron microscopy facility

 Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 914 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
Changed:
<
<		 b. How to adjust the eucentric height of the specimen
>
>
  1. How to adjust the eucentric height of the specimen
 
Changed:
<
<		 c. How to adjust the intensity of the beam
>
>
  1. How to adjust the intensity of the beam
 
Changed:
<
<		 d. How to focus the image
>
>
  1. How to focus the image
 
Changed:
<
<		 e. How to change magnification
>
>
  1. How to change magnification
 
Changed:
<
<		 f. How to introduce and center a condenser aperture
>
>
  1. How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:

  1. How to align the optical axis of the instrument

b. How to correct for condenser astigmatism

c. How to align the spot sizes

d. How to align the pivot points

e. How to introduce an objective aperture.

f. How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

b. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

c. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.

d. Refresh rate of the fast scan camera.

e. Diffraction mode. How to get electron diffraction patterns

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.

b. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

c. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

d. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as

  1. What to do in case the vacuum breaks

b. What to do if the beam shuts down

c. What to do if the lenses are not responding

d. What to do if there is water leaking

e. Precautions to be followed with liquid nitrogen

f. Precautions to be followed with liquid ethane

g. Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 814 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
Changed:
<
<
  1. How to get an electron beam
>
>
  1. How to get an electron beam
 
Changed:
<
<		 ii. How to adjust the eucentric height of the specimen
>
>		 b. How to adjust the eucentric height of the specimen
 
Changed:
<
<		 iii. How to adjust the intensity of the beam
>
>		 c. How to adjust the intensity of the beam
 
Changed:
<
<
  1. How to focus the image
>
>		 d. How to focus the image
 
Changed:
<
<
  1. How to change magnification
>
>		 e. How to change magnification
 
Changed:
<
<
  1. How to introduce and center a condenser aperture
>
>		 f. How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:
Changed:
<
<
  • a) How to align the optical axis of the instrument
>
>
  1. How to align the optical axis of the instrument
 
Changed:
<
<
  • b) How to correct for condenser astigmatism
>
>		 b. How to correct for condenser astigmatism
 
Changed:
<
<
  • c) How to align the spot sizes
>
>		 c. How to align the spot sizes
 
Changed:
<
<
  • d) How to align the pivot points
>
>		 d. How to align the pivot points
 
Changed:
<
<
  • e) How to introduce an objective aperture.
>
>		 e. How to introduce an objective aperture.
 
Changed:
<
<
  • f) How to correct for objective astigmatism
>
>		 f. How to correct for objective astigmatism
 

Advanced Setup of Microscope

After these alignments, the trainee will need to learn
Changed:
<
<
  • a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
>
>
  1. How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
 
Changed:
<
<
  • b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
>
>		 b. If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
 
Changed:
<
<
  • c) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
>
>		 c. Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
 
Changed:
<
<
  • d) Refresh rate of the fast scan camera.
>
>		 d. Refresh rate of the fast scan camera.
 
Changed:
<
<
  • e) Diffraction mode. How to get electron diffraction patterns
>
>		 e. Diffraction mode. How to get electron diffraction patterns
 

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

Changed:
<
<
  • a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
>
>
  1. Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
 
Changed:
<
<
  • b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
>
>		 b. Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
 
Changed:
<
<
  • c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
>
>		 c. Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
 
Changed:
<
<
  • d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
>
>		 d. Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
 

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as
Changed:
<
<
  • a) What to do in case the vacuum breaks
>
>
  1. What to do in case the vacuum breaks
 
Changed:
<
<
  • b) What to do if the beam shuts down
>
>		 b. What to do if the beam shuts down
 
Changed:
<
<
  • c) What to do if the lenses are not responding
>
>		 c. What to do if the lenses are not responding
 
Changed:
<
<
  • d) What to do if there is water leaking
>
>		 d. What to do if there is water leaking
 
Changed:
<
<
  • e) Precautions to be followed with liquid nitrogen
>
>		 e. Precautions to be followed with liquid nitrogen
 
Changed:
<
<
  • f) Precautions to be followed with liquid ethane
>
>		 f. Precautions to be followed with liquid ethane
 
Changed:
<
<
  • g) Etiquette of the electron microscopy facility
>
>		 g. Etiquette of the electron microscopy facility
 Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 713 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  1. How to get an electron beam
Changed:
<
<
  1. How to adjust the eucentric height of the specimen
>
>		 ii. How to adjust the eucentric height of the specimen
 
Changed:
<
<
  1. How to adjust the intensity of the beam
>
>		 iii. How to adjust the intensity of the beam
 
  1. How to focus the image

  1. How to change magnification

  1. How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  • a) How to align the optical axis of the instrument

  • b) How to correct for condenser astigmatism

  • c) How to align the spot sizes

  • d) How to align the pivot points

  • e) How to introduce an objective aperture.

  • f) How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  • a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

  • b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

  • c) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.

  • d) Refresh rate of the fast scan camera.

  • e) Diffraction mode. How to get electron diffraction patterns

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  • a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.

  • b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

  • c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

  • d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, information about common emergency procedures and precautions will be given, such as

  • a) What to do in case the vacuum breaks

  • b) What to do if the beam shuts down

  • c) What to do if the lenses are not responding

  • d) What to do if there is water leaking

  • e) Precautions to be followed with liquid nitrogen

  • f) Precautions to be followed with liquid ethane

  • g) Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 613 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
Changed:
<
<
  • a) How to get an electron beam
>
>
  1. How to get an electron beam
 
Changed:
<
<
  • b) How to adjust the eucentric height of the specimen
>
>
  1. How to adjust the eucentric height of the specimen
 
Changed:
<
<
  • c) How to adjust the intensity of the beam
>
>
  1. How to adjust the intensity of the beam
 
Changed:
<
<
  • d) How to focus the image
>
>
  1. How to focus the image
 
Changed:
<
<
  • e) How to change magnification
>
>
  1. How to change magnification
 
Changed:
<
<
  • f) How to introduce and center a condenser aperture
>
>
  1. How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:

  • a) How to align the optical axis of the instrument

  • b) How to correct for condenser astigmatism

  • c) How to align the spot sizes

  • d) How to align the pivot points

  • e) How to introduce an objective aperture.

  • f) How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  • a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

  • b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

  • c) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.

  • d) Refresh rate of the fast scan camera.

  • e) Diffraction mode. How to get electron diffraction patterns

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  • a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.

  • b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

  • c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

  • d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Changed:
<
<		Once these topics have been taught, emergency procedures will be taught, such as
>
>		Once these topics have been taught, information about common emergency procedures and precautions will be given, such as
 
  • a) What to do in case the vacuum breaks

  • b) What to do if the beam shuts down

  • c) What to do if the lenses are not responding

  • d) What to do if there is water leaking

  • e) Precautions to be followed with liquid nitrogen

  • f) Precautions to be followed with liquid ethane

  • g) Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 513 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

  • a) How to get an electron beam

  • b) How to adjust the eucentric height of the specimen

  • c) How to adjust the intensity of the beam

  • d) How to focus the image

  • e) How to change magnification

  • f) How to introduce and center a condenser aperture

General Setup of microscope

Then the trainee will need to learn the following:

  • a) How to align the optical axis of the instrument

  • b) How to correct for condenser astigmatism

  • c) How to align the spot sizes

  • d) How to align the pivot points

  • e) How to introduce an objective aperture.

  • f) How to correct for objective astigmatism

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

  • a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

  • b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

  • c) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.

  • d) Refresh rate of the fast scan camera.

  • e) Diffraction mode. How to get electron diffraction patterns

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

  • a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.

  • b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

  • c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

  • d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

Emergency Procedures

Once these topics have been taught, emergency procedures will be taught, such as

  • a) What to do in case the vacuum breaks

  • b) What to do if the beam shuts down

  • c) What to do if the lenses are not responding

  • d) What to do if there is water leaking

  • e) Precautions to be followed with liquid nitrogen

  • f) Precautions to be followed with liquid ethane
Changed:
<
<
  • g) *Etiquette of the electron microscopy facility*
>
>
  • g) Etiquette of the electron microscopy facility
 Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 413 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

Changed:
<
<		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The main requirements are the following:
>
>		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The topics to be covered are the following:
 

Absolute Basics of operation

The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
Changed:
<
<		a) How to get an electron beam
>
>
  • a) How to get an electron beam
 
Changed:
<
<		b) How to adjust the eucentric height of the specimen
>
>
  • b) How to adjust the eucentric height of the specimen
 
Changed:
<
<		c) How to adjust the intensity of the beam
>
>
  • c) How to adjust the intensity of the beam
 
Changed:
<
<		d) How to focus the image
>
>
  • d) How to focus the image
 
Changed:
<
<		e) How to change magnification
>
>
  • e) How to change magnification
 
Changed:
<
<		f) How to introduce and center a condenser aperture
>
>
  • f) How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:
Changed:
<
<		a) How to align the optical axis of the instrument
>
>
  • a) How to align the optical axis of the instrument
 
Changed:
<
<		b) How to correct for condenser astigmatism
>
>
  • b) How to correct for condenser astigmatism
 
Changed:
<
<		c) How to align the spot sizes
>
>
  • c) How to align the spot sizes
 
Changed:
<
<		d) How to align the pivot points
>
>
  • d) How to align the pivot points
 
Changed:
<
<		e) How to introduce an objective aperture.
>
>
  • e) How to introduce an objective aperture.
 
Changed:
<
<		f) How to correct for objective astigmatism
>
>
  • f) How to correct for objective astigmatism
 

Advanced Setup of Microscope

After these alignments, the trainee will need to learn
Changed:
<
<		a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
>
>
  • a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).
 
Changed:
<
<		b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
>
>
  • b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
 
Changed:
<
<		b) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
>
>
  • c) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
 
Changed:
<
<		c) Refresh rate of the fast scan camera.
>
>
  • d) Refresh rate of the fast scan camera.
 
Changed:
<
<		d) Diffraction mode. How to get electron diffraction patterns
>
>
  • e) Diffraction mode. How to get electron diffraction patterns
 

Sample Insertion and Removal

After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

Changed:
<
<		a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
>
>
  • a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.
 
Changed:
<
<		b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
>
>
  • b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.
 
Changed:
<
<		c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
>
>
  • c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.
 
Changed:
<
<		d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
>
>
  • d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.
 

Emergency Procedures

Once these topics have been taught, emergency procedures will be taught, such as
Changed:
<
<		a) What to do in case the vacuum breaks
>
>
  • a) What to do in case the vacuum breaks
 
Changed:
<
<		b) What to do if the beam shuts down
>
>
  • b) What to do if the beam shuts down
 
Changed:
<
<		c) What to do if the lenses are not responding
>
>
  • c) What to do if the lenses are not responding
 
Changed:
<
<		d) What to do if there is water leaking
>
>
  • d) What to do if there is water leaking
 
Changed:
<
<		e) Precautions to be followed with liquid nitrogen
>
>
  • e) Precautions to be followed with liquid nitrogen
 
Changed:
<
<		f) Precautions to be followed with liquid ethane
>
>
  • f) Precautions to be followed with liquid ethane
 
Changed:
<
<		g) Etiquette of the electron microscopy facility
>
>
  • g) *Etiquette of the electron microscopy facility*
 Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 313 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

Changed:
<
<		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. The main requirements are the following:
>
>		The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. To acquire all hour access, the user must have met all the requirements for 24 hour access to the NYSBC. The main requirements are the following:
 
Deleted:
<
<		1. The user must have met all the requirements for 24 hour access to the NYSBC.
 

Absolute Basics of operation

Changed:
<
<		2. The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.
>
>		The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron
Added:
>
>		microscope.
 a) How to get an electron beam

b) How to adjust the eucentric height of the specimen

c) How to adjust the intensity of the beam

d) How to focus the image

e) How to change magnification

Added:
>
>		f) How to introduce and center a condenser aperture
 

General Setup of microscope

Then the trainee will need to learn the following:
Changed:
<
<		a) How to put and center a condenser aperture
>
>		a) How to align the optical axis of the instrument
 
Changed:
<
<		b) How to align the optical axis of the instrument
>
>		b) How to correct for condenser astigmatism
 
Changed:
<
<		c) How to correct for condenser astigmatism
>
>		c) How to align the spot sizes
 d) How to align the pivot points

e) How to introduce an objective aperture.

f) How to correct for objective astigmatism

Deleted:
<
<
 

Advanced Setup of Microscope

After these alignments, the trainee will need to learn

a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.

Changed:
<
<		b) Flatfield correction for the CCD camera.
>
>		b) Flatfield correction for the CCD camera. Gain adjustments and other features of the CCD control program (EM menu). Film settings.
 
Changed:
<
<		c) Flatfield correction and refresh rate of the fast scan camera (I don't think flatfield collection for this camera is necessary for 24 hour users. Do we really want anyone else fiddling with this? -- Bill)
>
>		c) Refresh rate of the fast scan camera.
 d) Diffraction mode. How to get electron diffraction patterns
Changed:
<
<		After the training mentioned above, sample insertion will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.
>
>

Sample Insertion and Removal

 
Added:
>
>		After the training mentioned above, sample insertion and removal will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

a) Sample insertion and removal with the room temperature holder. Loading of negative stained grids, and their removal.

b) Use of the cryoholder at room temperature. Loading of specimen and procedure for insertion of cryoholder into the mocroscope.

c) Loading of frozen hydrated sample in the cryoholder and insertion into the microscope. Monitoring of temperature stability.

d) Vacuum state of the microscope. What values should be reading in the different gauges and what to do when vacuum is poor.

 

Emergency Procedures

Once these topics have been taught, emergency procedures will be taught, such as

a) What to do in case the vacuum breaks

b) What to do if the beam shuts down

c) What to do if the lenses are not responding

Added:
>
>		d) What to do if there is water leaking
 
Added:
>
>		e) Precautions to be followed with liquid nitrogen

f) Precautions to be followed with liquid ethane

g) Etiquette of the electron microscopy facility

Training will take place in ~5 sessions, with as many sessions for practice as needed. A practical exam will be administered to the users before they can be allowed to operate the instruments alone, keeping in mind that it is in everyone's best interest that the instruments are kept in a mint working condition, while maximizing the scientific productivity of all the users of the facility.

 
  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 212 Mar 2008 - Main.BillRice

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. The main requirements are the following:

1. The user must have met all the requirements for 24 hour access to the NYSBC.

Added:
>
>

Absolute Basics of operation

 2. The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

a) How to get an electron beam

b) How to adjust the eucentric height of the specimen

c) How to adjust the intensity of the beam

d) How to focus the image

e) How to change magnification

Added:
>
>

General Setup of microscope

 Then the trainee will need to learn the following:

a) How to put and center a condenser aperture

b) How to align the optical axis of the instrument

c) How to correct for condenser astigmatism

d) How to align the pivot points

e) How to introduce an objective aperture.

Added:
>
>		f) How to correct for objective astigmatism

Advanced Setup of Microscope

 After these alignments, the trainee will need to learn

a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

Added:
>
>		b) If necessary, how to set up SerialEM in low-dose mode. How to set up and collect a tilt series. Microscope alignments to be particularly careful about for proper operation of SerialEM.
 b) Flatfield correction for the CCD camera.
Changed:
<
<		c) Flatfield correction and refresh rate of the fast scan camera
>
>		c) Flatfield correction and refresh rate of the fast scan camera (I don't think flatfield collection for this camera is necessary for 24 hour users. Do we really want anyone else fiddling with this? -- Bill)
 d) Diffraction mode. How to get electron diffraction patterns

After the training mentioned above, sample insertion will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

Added:
>
>

Emergency Procedures

 Once these topics have been taught, emergency procedures will be taught, such as

a) What to do in case the vacuum breaks

b) What to do if the beam shuts down

c) What to do if the lenses are not responding

Changed:
<
<

subsub level topic

>
>
 
  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

Revision 112 Mar 2008 - Main.RubenDiaz

 
META TOPICPARENT name="CemfacGroup"
Contents

AllHourAccess?

How to use the microscopes at any hour of the day

The electron microscopes are in principle available for ~20 hours per day for data collection. However, due to the fact that staff is not available at all hours, several requirements need to be met before open access to the instruments can be granted. Open access will be given for individual instruments, as each instrument has slightly different requirements. The main requirements are the following:

1. The user must have met all the requirements for 24 hour access to the NYSBC.

2. The user must undergo several training sessions, approved by the NYSBC staff, about the use of the particular instrument for which the approval is sought. The training will begin with the basics of operation of an electron microscope.

a) How to get an electron beam

b) How to adjust the eucentric height of the specimen

c) How to adjust the intensity of the beam

d) How to focus the image

e) How to change magnification

Then the trainee will need to learn the following:

a) How to put and center a condenser aperture

b) How to align the optical axis of the instrument

c) How to correct for condenser astigmatism

d) How to align the pivot points

e) How to introduce an objective aperture.

After these alignments, the trainee will need to learn

a) How to set up the low dose kit of the microscope. Set up of search mode using the fast scan camera and/or binoculars; focus mode at a suitably high magnification; set up of the proper imaging conditions (electron dose to be given to the sample, exposure time required).

b) Flatfield correction for the CCD camera.

c) Flatfield correction and refresh rate of the fast scan camera

d) Diffraction mode. How to get electron diffraction patterns

After the training mentioned above, sample insertion will be taught. The reasoning for this, is that sample insertion is the most frequent cause of vacuum breaches and goniometer breakdowns, therefore, it is desirable to leave it at the end, for those users that have already acquired familiarity with the instrument.

Once these topics have been taught, emergency procedures will be taught, such as

a) What to do in case the vacuum breaks

b) What to do if the beam shuts down

c) What to do if the lenses are not responding

subsub level topic

  • Set ALLOWTOPICVIEW =

-- RubenDiaz - 12 Mar 2008

View topic  |  History: r19 < r18 < r17 < r16  |  More topic actions...
 
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