Difference: ArthurPalmer (20 vs. 21)

• Name: Arthur Palmer
• Email: agp@nysbc.org
• Company Name: Columbia Univ. Medical Center
• Company URL: http://www.palmer.hs.columbia.edu
• Location: ColumbiaMedCenterOffice

• Country: USA

Grants associated with NYSBC

GM59273

1. GM 59273 5/1/02-4/30/06; 5/1/06-4/30/10 has been awarded CRISP UPDATE PENDING

GM50291

Grant Number: 2R01GM050291-12 PI Name: PALMER, ARTHUR G. PI Email: agp6@columbia.edu PI Title: PROFESSOR Project Title: Nuclear Magnetic Resonance Studies of Proteins

Abstract: DESCRIPTION (provided by applicant): Comprehensive experimental information on the essential contributions of intramolecular dynamics to biological functions of proteins is critical for biophysical theories of equilibrium properties, such as heat capacity and thermal stability; for mechanistic interpretations of kinetic processes, such as enzyme catalysis and ligand recognition; and for design of novel proteins and protein ligands, including pharmaceutical agents. This research project will use multidimensional NMR spectroscopy to address these fundamental issues. One long-term goal is to define the molecular determinants of stability and catalytic activity of the enzyme ribonuclease HI (RNase H) by comparing the structural, dynamical and enzymatic properties of homologous proteins derived from Escherichia coli and the extremely thermophilic bacterium Thermus thermophilus. The enzyme is distributed widely in prokaryotes and eukaryotes, and retroviral reverse transcriptase contains a C-terminal RNase H domain. Another long-term goal is to define the molecular determinants of ligand binding, including aspects of specificity, and allosterism, in DNA recognition by the yeast protein GCN4 and in nucleotide binding by the ATP-binding cassette (ABC) MJ1267 of the branched chain amino acid (LIV) transporter from Methanococcus jannaschii. GCN4 is the prototypical member of the bZip family of transcription activators. Motifs that recognize specific DNA sequences are ubiquitous components of proteins that regulate gene expression; consequently, explication of the molecular basis for recognition is critical for understanding normal biological function and pathology. GCN4 represents an example of induced fit molecular recognition through a disorder-order transition associated with DNA binding. The LIV transporter is a member of a diverse family of ABC transporters involved in numerous biological processes and diseases, including multidrug resistance and cystic fibrosis. ABC MJ1267 represents an example of selected-fit molecular recognition and allosteric transmission of conformational changes between remote sites in proteins.

Thesaurus Terms: bacterial protein, enzyme activity, intermolecular interaction, molecular dynamics, protein structure function, ribonuclease H, structural biology, thermostability adenosine diphosphate, adenosine triphosphate, biophysics, chemical kinetics, enzyme substrate complex, membrane transport protein, molecular site, nucleic acid, protein folding, species difference, temperature, transcription factor Archaea, Escherichia coli, nuclear magnetic resonance spectroscopy, site directed mutagenesis

Institution: COLUMBIA UNIVERSITY HEALTH SCIENCES PO Box 49 NEW YORK, NY 10032 Fiscal Year: 2005 Department: BIOCHEM & MOLECULAR BIOPHYSICS Project Start: 01-AUG-1994 Project End: 31-JUL-2009 ICD: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES IRG: MSFC

• Short summary.

Personal Preferences (details in TWikiVariables)

• Horizontal size of text edit box:
  • Set EDITBOXWIDTH = 70
• Vertical size of text edit box:
  • Set EDITBOXHEIGHT = 17
• Style of text edit box. width: 99% for full window width (default), width: auto to disable.
  • Set EDITBOXSTYLE = width: 99%
• Optionally write protect your home page: (set it to your WikiName)
  • Set ALLOWTOPICCHANGE =

Related topics

• TWikiPreferences has site-level preferences of TWiki.
• WebPreferences has preferences of the TWiki.Main web.
• TWikiUsers has a list of other TWiki users.
• Palmer.jpg: