
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicDMPC + C12E8 room temp (April 25, 2011)DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight.DMPC + DDM room temp (April 27, 2011)DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.DMPC + DDM 4 degrees (April 29, 2011)DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.Egg PC:PA + C12E8 room temp (April 30, 2011)1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs.
| ||||||||
| Added: | ||||||||
| > > | * bb_uma_yiip.pptx: BioBeads?_UMA_YiiP_for_separation | |||||||
| ||||||||
| Added: | ||||||||
| > > |
| |||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopic | ||||||||
| Changed: | ||||||||
| < < | DMPC + C12E8 (April 25, 2011) | |||||||
| > > | DMPC + C12E8 room temp (April 25, 2011) | |||||||
DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight.
DMPC + DDM room temp (April 27, 2011)DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.DMPC + DDM 4 degrees (April 29, 2011)DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | ||||||||
| Changed: | ||||||||
| < < | Egg PC:PA + C12E8 (April 30, 2011) | |||||||
| > > | Egg PC:PA + C12E8 room temp (April 30, 2011) | |||||||
1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs.
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicDMPC + C12E8 (April 25, 2011)DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight. | ||||||||
| Changed: | ||||||||
| < < | DMPC + DDM (April 27, 2011) | |||||||
| > > | DMPC + DDM room temp (April 27, 2011) | |||||||
| DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | ||||||||
| Changed: | ||||||||
| < < | DMPC + DDM (April 29, 2011) | |||||||
| > > | DMPC + DDM 4 degrees (April 29, 2011) | |||||||
| DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | ||||||||
| Changed: | ||||||||
| < < | April 30, 2011 | |||||||
| > > | Egg PC:PA + C12E8 (April 30, 2011) | |||||||
1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs.
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopic | ||||||||
| Changed: | ||||||||
| < < | April 25, 2011 | |||||||
| > > | DMPC + C12E8 (April 25, 2011) | |||||||
| DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight. | ||||||||
| Changed: | ||||||||
| < < | April 27, 2011 | |||||||
| > > | DMPC + DDM (April 27, 2011) | |||||||
| DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | ||||||||
| Changed: | ||||||||
| < < | April 29, 2011 | |||||||
| > > | DMPC + DDM (April 29, 2011) | |||||||
DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.
April 30, 20111 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs.
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight.April 27, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.April 29, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.April 30, 20111 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs.
|
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight.April 27, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.April 29, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs.April 30, 2011 | ||||||||
| Changed: | ||||||||
| < < | 1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize EY PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs. | |||||||
| > > | 1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize eggyolk PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight. | |||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were distributed into a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight. | |||||||
April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
April 29, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed into a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
April 30, 2011 | ||||||||
| Changed: | ||||||||
| < < | 1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize EY PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs. | |||||||
| > > | 1 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize EY PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed into a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight. | |||||||
April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
April 29, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs. | |||||||
| Added: | ||||||||
| > > |
April 30, 20111 mg of eggyolk PC:PA (9:1) was mixed with C12E8 (1 ml of 0.05 mg/ml). 160 ul of 5 mg/ml C12E8 (~0.8 mg total) was added to completely solubilize EY PC:PA. 100 ul aliquots of the EY PC:PA - C12E8 mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 16 beads (~4 mg) per well and incubated (room temp) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 24 hrs, 48 hrs. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively.April 29, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 170 ul of 5 mg/ml DDM (~0.85 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| ||||||||
| Added: | ||||||||
| > > | ||||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively.April 29, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 170 ul of 5 mg/ml DDM (~0.85 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively.
| ||||||||
| Added: | ||||||||
| > > |
| |||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | ||||||||
| Added: | ||||||||
| > > |
April 29, 2011DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 170 ul of 5 mg/ml DDM (~0.85 mg total) was added to completely solubilize DMPC (0.001 light scattering at 500 nm). 100 ul aliquots of the DMPC-DDM mixture were distributed in 10 wells of a 96-well plate. A1 - A10 had 8 beads (~2 mg) per well and incubated (in fridge, 4 degrees) for 15 minutes, 30 minutes, 1 hr, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, respectively. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were distributed in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg total) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| Added: | ||||||||
| > > | ||||||||
April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM (~0.75 mg total) was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
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Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 7 hrs, and overnight, respectively.. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 1.5 hrs, 2 hrs, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 24 hrs, 48 hrs, and 62 hrs, respectively.. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.April 27, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 7 hrs, and overnight, respectively.. | |||||||
| > > | DMPC (1 mg) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 7 hrs, and overnight, respectively.. | |||||||
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Detergent Removal with Biobeads - ExperimentsContentsTopicSubtopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 30 ul of 5 mg/ml C12E8 (~0.2 mg) was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in a 96-well plate. A1 - A4 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (~1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| Added: | ||||||||
| > > | April 27, 2011DMPC (1 mg vacuum-dried film) was mixed with DDM (1 ml of 0.05 mg/ml). 150 ul of 5 mg/ml DDM was added to completely solubilize DMPC. 100 ul aliquots of the DMPC-DDM mixture were prepared in a 96-well plate. H1 - H11 had 8 beads (~2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, 4 hrs, 5 hrs, 6 hrs, 7 hrs, and overnight, respectively.. | |||||||
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| Changed: | ||||||||
| < < | -- RalphLasala - 26 Apr 2011 | |||||||
| > > | -- RalphLasala - 27 Apr 2011 | |||||||
Detergent Removal with Biobeads - ExperimentsContentsTopic | ||||||||
| Changed: | ||||||||
| < < | April 25, 2011sub-subtopic | |||||||
| > > | SubtopicApril 25, 2011 | |||||||
DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| > > | sub-subtopic | |||||||
| Changed: | ||||||||
| < < | ||||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsTopicApril 25, 2011DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively.
|
Detergent Removal with Biobeads - ExperimentsContentsTopicApril 25, 2011 | ||||||||
| Changed: | ||||||||
| < < | subsub level topic | |||||||
| > > | DMPC (1 mg vacuum-dried film) was mixed with C12E8 (1 ml of 0.05 mg/ml). A total of 0.2 mg C12E8 was added to completely solubilize DMPC (6 additions of 5 ul of 5 mg/ml C12E8). 100 ul aliquots of the DMPC-C12E8 mixture were prepared in 96-well plate. A1 - A4 had 8 beads (2 mg) per well and incubated (room temp with shaking) for 30 minutes, 1 hr, 3 hrs, and overnight, respectively. B1-B4 had 4 beads (1 mg) per well and incubated for for 30 minutes, 1 hr, and overnight, respectively. | |||||||
| Deleted: | ||||||||
| < < | ||||||||
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Detergent Removal with Biobeads - ExperimentsContents | ||||||||
| Changed: | ||||||||
| < < | April 25, 2011Sublevel topic | |||||||
| > > | TopicApril 25, 2011 | |||||||
subsub level topic
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Detergent Removal with Biobeads - ExperimentsContents | ||||||||
| Changed: | ||||||||
| < < | Name of the topic | |||||||
| > > | April 25, 2011 | |||||||
Sublevel topicsubsub level topic
| ||||||||
Detergent Removal with Biobeads - ExperimentsContentsName of the topicSublevel topicsubsub level topic
|