Nikon Ti-U Description

Correlative Light and Electron Microscopy (CLEM)

Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm).

Equipment

Nikon Ti-U Research Fluorescence Microscope System Specifications

Light microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted Illuminator

Brightfield: Diascopic Tilt Back Illuminator
Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module

Camera: Photometrics HQ2 Digital Camera

CFI60 Infinity Objective Lenses
CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L
CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2)
CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C)

CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI

with an Intermediate Magnification Changer 1x - 1.5x

Stage: Custom stage to accommodate the FEI Cryo Stage2 System

FEI Cryo Stage2

The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.

* CryoStage_Manual_Final_V1_1.pdf: FEI Cryo Stage 2 Manual

• Set ALLOWTOPICVIEW =