Difference: CemNikonEclipse (1 vs. 17)
Revision 1721 Mar 2014 - Main.EdEng
Nikon Ti-U Description
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm).EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera | |||||||||||
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| > > | CCD: 1392 x 1040 px 6.45-µm pixel size | ||||||||||
| CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System FEI Cryo Stage2
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Revision 1620 Sep 2012 - Main.EdEng
Nikon Ti-U Description
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Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | |||||||||||
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EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System FEI Cryo Stage2
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Revision 1520 Sep 2012 - Main.EdEng
Nikon Ti-U Description
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| < < | *Basic Instructions* | |||||||||||
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Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm).EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System FEI Cryo Stage2
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Revision 1420 Sep 2012 - Main.EdEng
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| < < | Correlative Light and Electron Microscopy (CLEM) | ||||||||
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| < < | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3 | ||||||||
Nikon Ti-U Description
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Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||
EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System FEI Cryo Stage2
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Revision 1320 Sep 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3 | |||||||||
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EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System FEI Cryo Stage2
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| > > | * CryoStage_Manual_Final_V1_1.pdf: FEI Cryo Stage 2 Manual | ||||||||
Basic Instructions
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Revision 1219 Sep 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup | |||||||||||
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| Users have access to a Nikon Ti-U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by scanning electron microscope (SEM) or transmission electron microscope (TEM). The microscope is outfitted with 4 objective lenses with phase contrast: 4x, 20x, 40x and 50x with an inline 1.5x magnification to allow 6x, 30x, 60x and 75x magnifications. Also, we have a homemade liquid nitrogen gravity dosing system to maintain the temperature of the system below -150C for imaging. All 4 objective lenses are custom fitted to be compatible for imaging (without removal) when the FEI Cryo Stage2 mounted. | |||||||||||
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EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System | |||||||||||
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| < < | The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. | ||||||||||
FEI Cryo Stage2
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Revision 1128 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM) | |||||||||||
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| < < | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining Cryo EM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||||
| > > | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||||
EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2
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Revision 1028 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining Cryo EM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup | ||||||||||||
| Changed: | ||||||||||||
| < < | Users have access to a Nikon Ti-U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by scanning electron microscope (SEM) or transmission electron microscope (TEM). The microscope is outfitted with 4 objective lenses with phase contrast: 4x, 20x, 40x and 50x with an inline 1.5x magnification to allow 6x, 30x, 60x and 75x magnifications. Also, we have a homemade liquid nitrogen gravity dosing system to maintain the temperature of the system below -150C for imaging. | |||||||||||
| > > | Users have access to a Nikon Ti-U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by scanning electron microscope (SEM) or transmission electron microscope (TEM). The microscope is outfitted with 4 objective lenses with phase contrast: 4x, 20x, 40x and 50x with an inline 1.5x magnification to allow 6x, 30x, 60x and 75x magnifications. Also, we have a homemade liquid nitrogen gravity dosing system to maintain the temperature of the system below -150C for imaging. All 4 objective lenses are custom fitted to be compatible for imaging (without removal) when the FEI Cryo Stage2 mounted. | |||||||||||
EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2
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Revision 927 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining Cryo EM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup | |||||||||||
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| < < | * nikonmicroscope.JPG: | ||||||||||
EquipmentNikon Ti-U Research Fluorescence Microscope System SpecificationsLight microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted IlluminatorBrightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x Stage: Custom stage to accommodate the FEI Cryo Stage2 System | |||||||||||
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| < < | * FEI cryostage picture: | ||||||||||
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2 | |||||||||||
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| < < | * CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual | ||||||||||
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Revision 826 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining Cryo EM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup* nikonmicroscope.JPG:
Users have access to a Nikon Ti-U inverted fluorescence microscope with a FEI Cryo Stage2 system. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by scanning electron microscope (SEM) or transmission electron microscope (TEM). The microscope is outfitted with 4 objective lenses with phase contrast: 4x, 20x, 40x and 50x with an inline 1.5x magnification to allow 6x, 30x, 60x and 75x magnifications. Also, we have a homemade liquid nitrogen gravity dosing system to maintain the temperature of the system below -150C for imaging.
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Equipment | |||||||||||
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| < < | Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage | ||||||||||
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| Deleted: | |||||||||||
| < < | Smart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module | ||||||||||
Nikon Ti-U Research Fluorescence Microscope System Specifications | |||||||||||
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| < < | Photometrics HQ2 Digital Camera | ||||||||||
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| < < | Intermediate Magnification Changer 1x - 1.5x | ||||||||||
| > > | Light microscope: Nikon Eclipse Ti-U with Nikon Encoded Motorized Stage and Smart Shutter for Transmitted Illuminator | ||||||||||
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| > > | Brightfield: Diascopic Tilt Back Illuminator | ||||||||||
| Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module | |||||||||||
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| < < | CFI60 Infinity Objective Lenses | ||||||||||
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| Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) | |||||||||||
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| < < | Custom stage to accommodate the FEI CryoStage2? System | ||||||||||
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| > > | CRL Plan Epi 50x with custom 50x Modulator for CRL Plan EPI with an Intermediate Magnification Changer 1x - 1.5x | ||||||||||
| Stage: Custom stage to accommodate the FEI Cryo Stage2 System * FEI cryostage picture:
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2* CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual
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Revision 726 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM) | |||||||||||
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| < < | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy can combine sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||||
| > > | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures of cells. However, fluorescent microscopy methods lacks the fine structural information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining Cryo EM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy combines sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||||
EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System Stage: Custom stage to accommodate the FEI Cryo Stage2 System * FEI cryostage picture:
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2* CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual
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Revision 626 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy can combine sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup* nikonmicroscope.JPG: | |||||||||||
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EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System Stage: Custom stage to accommodate the FEI Cryo Stage2 System * FEI cryostage picture: | |||||||||||
| Changed: | |||||||||||
| < < | | ||||||||||
| > > | | ||||||||||
The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes.
FEI Cryo Stage2* CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual
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Revision 526 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy can combine sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). d13 3NYSBC setup | |||||||||||
| Added: | |||||||||||
| > > | * nikonmicroscope.JPG: | ||||||||||
EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System Stage: Custom stage to accommodate the FEI Cryo Stage2 System | |||||||||||
| Changed: | |||||||||||
| < < | The CryoStage2? System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. | ||||||||||
| > > | * FEI cryostage picture: | ||||||||||
| Added: | |||||||||||
| > > | | ||||||||||
| Added: | |||||||||||
| > > | The Cryo Stage2 System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. | ||||||||||
FEI Cryo Stage2 | |||||||||||
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| < < |
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* CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual
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Revision 426 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM) | |||||||||||
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| < < | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and would yield details on the order of vesicles and parts of organelles. | ||||||||||
| > > | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and a single fluorescent spot may arise from several vesicles or parts of organelles. Combining CryoEM techniques with light microscopy allows for localization of a wider spectrum of molecules against the structural background of a well preserved cellular architecture. Correlative Light and Electron Microscopy can combine sensitive protein-detection methods with detailed information on the substructure of intracellular compartments in the intermediate resolution range (0.5-2.0nm). | ||||||||||
EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System Stage: Custom stage to accommodate the FEI Cryo Stage2 System The CryoStage2? System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. FEI Cryo Stage2
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| > > | * CryoStage_Manual_Final_V1_1.pdf: CryoStage?_Manual_Final_V1_1.pdf
a47 2
* CryoStage_Manual_Final_V1_1.pdf: FEI CryoStage? 2 Manual
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Revision 326 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM) | ||||||||
| Added: | ||||||||
| > > | Use of fluorescent proteins to image living cells is a powerful technique to localize proteins and examine substructures. However, fluorescent-microscopy methods lacks fine structure information to interpret many of these signals. The resolution of light-microscopy based methods is around 200-500nm and would yield details on the order of vesicles and parts of organelles. | |||||||
| d13 3 | ||||||||
| Added: | ||||||||
| > > | NYSBC setup | |||||||
| Added: | ||||||||
| > > | ||||||||
EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System | ||||||||
| Changed: | ||||||||
| < < | FEI CryoStage2? | |||||||
| > > | Stage: Custom stage to accommodate the FEI Cryo Stage2 System | |||||||
| Changed: | ||||||||
| < < | The CryoStage2? System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by transmission electron microscopy (TEM). | |||||||
| > > | The CryoStage2? System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. | |||||||
FEI Cryo Stage2
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Revision 226 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)d13 3 | ||||||||
| Added: | ||||||||
| > > | EquipmentNikon Eclipse Ti-U with Nikon Encoded Motorized StageSmart Shutter for Transmitted Illuminator Brightfield: Diascopic Tilt Back Illuminator Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module Nikon Ti-U Research Fluorescence Microscope System SpecificationsPhotometrics HQ2 Digital CameraIntermediate Magnification Changer 1x - 1.5x Fluorescence: INTENSILIGHT 130w Mercury Illuminator with Epi-Fluorescence Module CFI60 Infinity Objective Lenses Camera: Photometrics HQ2 Digital Camera CFI60 Infinity Objective Lenses CFI Plan Fluor DL 4x / 0.13 / 16.50 Phase L CFI ELWD Semi-APO ADM 20x/.45/8.2 Phase 1 (Corr 0-2) CFI ELWD Semi-APO ADM 40x/0.6/3.6 Phase 2 (C) Custom stage to accommodate the FEI CryoStage2? System FEI CryoStage2?The CryoStage2? System (the correlative stage for microscopy) is a liquid nitrogen cooled microscope stage which can be used on inverted fluorescence research microscopes. It allows screening of electron microscopy grids by means of light microscopy prior to an investigation by transmission electron microscopy (TEM).FEI Cryo Stage2 | |||||||
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Revision 126 Jun 2012 - Main.EdEng
Correlative Light and Electron Microscopy (CLEM)d13 3
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